Thulium Laser in the Upper Urinary Tract: Does the Heat Generation in the Irrigation Fluid Pose a Risk? Evidence from an In Vivo Experimental Study

2016 ◽  
Vol 30 (5) ◽  
pp. 555-559 ◽  
Author(s):  
Panagiotis Kallidonis ◽  
Wissam Kamal ◽  
Vasileios Panagopoulos ◽  
Marinos Vasilas ◽  
Lefteris Amanatides ◽  
...  
2016 ◽  
Vol 30 (4) ◽  
pp. 422-427 ◽  
Author(s):  
Panagiotis Kallidonis ◽  
Lefteris Amanatides ◽  
Vasileios Panagopoulos ◽  
Iason Kyriazis ◽  
Theofanis Vrettos ◽  
...  

2015 ◽  
Vol 33 (4) ◽  
pp. 595-597 ◽  
Author(s):  
Esteban Emiliani ◽  
Thomas R. W. Herrmann ◽  
Alberto Breda

2021 ◽  
Vol 73 (1) ◽  
Author(s):  
Giorgio BOZZINI ◽  
Christian GASTALDI ◽  
Umberto BESANA ◽  
Alberto CALORI ◽  
Stefano CASELLATO ◽  
...  

2009 ◽  
Vol 24 (2) ◽  
pp. 128-129
Author(s):  
Ulrike Müller-Lisse ◽  
Markus Bader ◽  
Jasmin Hocaoglu ◽  
Michaela Püls ◽  
Ronald Sroka ◽  
...  

2018 ◽  
Vol 32 (3) ◽  
pp. 257-263 ◽  
Author(s):  
Gennaro Musi ◽  
Francesco A. Mistretta ◽  
Carlo Marenghi ◽  
Andrea Russo ◽  
Michele Catellani ◽  
...  

2020 ◽  
Author(s):  
Silvia Proietti ◽  
Moises Elias Rodríguez-Socarrás ◽  
Brian Howard Eisner ◽  
Roberta Lucianò ◽  
Mario José Basulto Martinez ◽  
...  

2014 ◽  
Vol 82 (9) ◽  
pp. 3644-3656 ◽  
Author(s):  
Michael D. Engstrom ◽  
Christopher J. Alteri ◽  
Harry L. T. Mobley

ABSTRACTA heterogeneous subset of extraintestinal pathogenicEscherichia coli(ExPEC) strains, referred to as uropathogenicE. coli(UPEC), causes most uncomplicated urinary tract infections. However, no core set of virulence factors exists among UPEC strains. Instead, the focus of the analysis of urovirulence has shifted to studying broad classes of virulence factors and the interactions between them. For example, the RTX nonfimbrial adhesin TosA mediates adherence to host cells derived from the upper urinary tract. The associatedtosoperon is well expressedin vivobut poorly expressedin vitroand encodes TosCBD, a predicted type 1 secretion system. TosR and TosEF are PapB and LuxR family transcription factors, respectively; however, no role has been assigned to these potential regulators. Thus, the focus of this study was to determine how TosR and TosEF regulatetosAand affect the reciprocal expression of adhesins and flagella. Among a collection of sequenced UPEC strains, 32% (101/317) were found to encode TosA, and nearly all strains (91% [92/101]) simultaneously carried the putative regulatory genes. Deletion oftosRalleviatestosArepression. Thetospromoter was localized upstream oftosRusing transcriptional fusions of putative promoter regions withlacZ. TosR binds to this region, affecting a gel shift. A 100-bp fragment 220 to 319 bp upstream oftosRinhibits binding, suggesting localization of the TosR binding site. TosEF, on the other hand, downmodulate motility when overexpressed by preventing the expression offliC, encoding flagellin. Deletion oftosEFincreased motility. Thus, we present an additional example of the reciprocal control of adherence and motility.


2011 ◽  
Vol 77 (19) ◽  
pp. 6852-6857 ◽  
Author(s):  
Gabriela Hidalgo ◽  
Michelle Chan ◽  
Nathalie Tufenkji

ABSTRACTIn humans, uropathogenicEscherichia coli(UPEC) is the most common etiological agent of uncomplicated urinary tract infections (UTIs). Cranberry extracts have been linked to the prevention of UTIs for over a century; however, a mechanistic understanding of the way in which cranberry derivatives prevent bacterial infection is still lacking. In this study, we used afliC-luxreporter as well as quantitative reverse transcription-PCR to demonstrate that when UPEC strain CFT073 was grown or exposed to dehydrated, crushed cranberries or to purified cranberry-derived proanthocyanidins (cPACs), expression of the flagellin gene (fliC) was inhibited. In agreement with these results, transmission electron microscopy imaging of bacteria grown in the presence of cranberry materials revealed fewer flagella than those in bacteria grown under control conditions. Furthermore, we showed that swimming and swarming motilities were hindered when bacteria were grown in the presence of the cranberry compounds. Because flagellum-mediated motility has been suggested to enable UPEC to disseminate to the upper urinary tract, we propose that inhibition of flagellum-mediated motility might be a key mechanism by which cPACs prevent UTIs. This is the first report to show that cranberry compounds inhibit UPEC motility via downregulation of thefliCgene. Further studies are required to establish whether these inhibitors play a rolein vivo.


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