Second Prize:Comprehensive Proteomic Analysis of Human Calcium Oxalate Monohydrate Kidney Stone Matrix

2008 ◽  
Vol 22 (6) ◽  
pp. 1161-1168 ◽  
Author(s):  
Benjamin K. Canales ◽  
Lorraine Anderson ◽  
Leeann Higgins ◽  
Joel Slaton ◽  
Ken P. Roberts ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Proteomic Analysis ◽  
Kidney Stone ◽  
Calcium Oxalate Monohydrate ◽  
Stone Matrix

scholarly journals 1633: Comprehensive Proteomic Analysis of Calcium Oxalate Monohydrate Stone Matrix

2007 ◽  
Vol 177 (4S) ◽  
pp. 542-542
Author(s):  
Benjamin K. Canales ◽  
Lorraine Anderson ◽  
LeeAnn Higgins ◽  
Joel W. Slaton ◽  
Nathan Liu ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Proteomic Analysis ◽  
Calcium Oxalate Monohydrate ◽  
Stone Matrix

scholarly journals Proteomic analysis reveals some common proteins in the kidney stone matrix

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11872
Author(s):  
Yuanyuan Yang ◽  
Senyuan Hong ◽  
Cong Li ◽  
Jiaqiao Zhang ◽  
Henglong Hu ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Kidney Stone ◽  
Renal Tissue ◽  
Matrix Proteins ◽  
Complement C3 ◽  
Urinary Stones ◽  
Protein Z ◽  
Kegg Analysis ◽  
Significant Difference ◽  
Stone Matrix

Background Proteins are the most abundant component of kidney stone matrices and their presence may reflect the process of the stone’s formation. Many studies have explored the proteomics of urinary stones and crystals. We sought to comprehensively identify the proteins found in kidney stones and to identify new, reliable biomolecules for use in nephrolithiasis research. Methods We conducted bioinformatics research in November 2020 on the proteomics of urinary stones and crystals. We used the ClusterProfiler R package to transform proteins into their corresponding genes and Ensembl IDs. In each study we located where proteomic results intersected to determine the 20 most frequently identified stone matrix proteins. We used the Human Protein Atlas to obtain the biological information of the 20 proteins and conducted Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome (KEGG) analysis to explore their biological functions. We also performed immunohistochemistry to detect the expression of the top five stone matrix proteins in renal tissue. Results We included 19 relevant studies for analysis. We then identified 1,409 proteins in the stone matrix after the duplicates were removed. The 20 most-commonly identified stone matrix proteins were: S100A8, S100A9, uromodulin, albumin, osteopontin, lactotransferrin, vitamin K-dependent protein Z, prothrombin, hemoglobin subunit beta, myeloperoxidase, mannan-binding lectin serine protease 2, lysozyme C, complement C3, serum amyloid P-component, cathepsin G, vitronectin, apolipoprotein A-1, eosinophil cationic protein, fibrinogen alpha chain, and apolipoprotein D. GO and KEGG analysis revealed that these proteins were typically engaged in inflammation and immune response.Immunohistochemistry of the top five stone matrix proteins in renal tissue showed that the expression of S100A8, S100A9, and osteopontin increased, while uromodulin decreased in kidney stone patients. Albumin was rarely expressed in the kidney with no significant difference between healthy controls and kidney stone patients. Conclusion Proteomic analysis revealed some common inflammation-related proteins in the kidney stone matrix. The role of these proteins in stone formation should be explored for their potential use as diagnostic biomarkers and therapeutic targets for urolithiasis.

Proteomic analysis of a urinary stone with two layers composed of calcium oxalate monohydrate and uric acid

2018 ◽  
Vol 37 (12) ◽  
pp. 717-723 ◽  
Author(s):  
Kiyoko Kaneko ◽  
Mizuho Kabeya ◽  
Hirokazu Kondo ◽  
Tomoko Fukuuchi ◽  
Noriko Yamaoka ◽  
...  
Keyword(s):  
Uric Acid ◽  
Calcium Oxalate ◽  
Proteomic Analysis ◽  
Calcium Oxalate Monohydrate ◽  
Urinary Stone
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