Anaerobic Bioremediation Performance and Indigenous Microbial Communities in Treatment of Trichloroethylene/Nitrate-Contaminated Groundwater

2018 ◽  
Vol 35 (4) ◽  
pp. 311-322 ◽  
Author(s):  
Shanshan Dong ◽  
Chuanping Feng ◽  
Weihua Cui ◽  
Nan Chen ◽  
Ying Liu ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Contaminated Groundwater ◽  
Anaerobic Bioremediation

scholarly journals A metagenomic approach to decipher the indigenous microbial communities of arsenic contaminated groundwater of Assam

Genomics Data ◽  
2017 ◽  
Vol 12 ◽  
pp. 89-96 ◽  
Author(s):  
Saurav Das ◽  
Sudipta Sankar Bora ◽  
R.N.S. Yadav ◽  
Madhumita Barooah
Keyword(s):  
Microbial Communities ◽  
Contaminated Groundwater ◽  
Metagenomic Approach

scholarly journals Characterization of microbial communities in the aqueous phase of a constructed model wetland treating 1,2-dichloroethene-contaminated groundwater

2010 ◽  
Vol 72 (1) ◽  
pp. 74-88 ◽  
Author(s):  
Gwenaël Imfeld ◽  
Cristian Estop Aragonés ◽  
Ingo Fetzer ◽  
Éva Mészáros ◽  
Simone Zeiger ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Aqueous Phase ◽  
Contaminated Groundwater

scholarly journals The impact of iron nanoparticles on technetium-contaminated groundwater and sediment microbial communities

2019 ◽  
Vol 364 ◽  
pp. 134-142 ◽  
Author(s):  
Laura Newsome ◽  
Katherine Morris ◽  
Adrian Cleary ◽  
Nicholas Karl Masters-Waage ◽  
Christopher Boothman ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Iron Nanoparticles ◽  
Contaminated Groundwater ◽  
The Impact

Hydrocarbon degrading microbial communities in bench scale aerobic biobarriers for gasoline contaminated groundwater treatment

Chemosphere ◽  
2015 ◽  
Vol 130 ◽  
pp. 34-39 ◽  
Author(s):  
Matteo Daghio ◽  
Valeria Tatangelo ◽  
Andrea Franzetti ◽  
Isabella Gandolfi ◽  
Maddalena Papacchini ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Contaminated Groundwater ◽  
Groundwater Treatment ◽  
Bench Scale

Comparison of microbial communities in petroleum-contaminated groundwater using genetic and metabolic profiles at Kyonggi-Do, South Korea

2009 ◽  
Vol 60 (2) ◽  
pp. 371-382 ◽  
Author(s):  
Eun-Hee Lee ◽  
Jaisoo Kim ◽  
Ji-Young Kim ◽  
So-Yeon Koo ◽  
Sang-Dong Lee ◽  
...  
Keyword(s):  
South Korea ◽  
Microbial Communities ◽  
Contaminated Groundwater ◽  
Metabolic Profiles

scholarly journals Lateral Gene Transfer in a Heavy Metal-Contaminated-Groundwater Microbial Community

mBio ◽  
2016 ◽  
Vol 7 (2) ◽  
Author(s):  
Christopher L. Hemme ◽  
Stefan J. Green ◽  
Lavanya Rishishwar ◽  
Om Prakash ◽  
Angelica Pettenato ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Microbial Community ◽  
Gene Transfer ◽  
Microbial Communities ◽  
Lateral Gene Transfer ◽  
Metal Contamination ◽  
Heavy Metal Contamination ◽  
Contaminated Groundwater ◽  
Rrna Gene ◽  
Changing Environments

ABSTRACT Unraveling the drivers controlling the response and adaptation of biological communities to environmental change, especially anthropogenic activities, is a central but poorly understood issue in ecology and evolution. Comparative genomics studies suggest that lateral gene transfer (LGT) is a major force driving microbial genome evolution, but its role in the evolution of microbial communities remains elusive. To delineate the importance of LGT in mediating the response of a groundwater microbial community to heavy metal contamination, representative Rhodanobacter reference genomes were sequenced and compared to shotgun metagenome sequences. 16S rRNA gene-based amplicon sequence analysis indicated that Rhodanobacter populations were highly abundant in contaminated wells with low pHs and high levels of nitrate and heavy metals but remained rare in the uncontaminated wells. Sequence comparisons revealed that multiple geochemically important genes, including genes encoding Fe 2+ /Pb 2+ permeases, most denitrification enzymes, and cytochrome c 553 , were native to Rhodanobacter and not subjected to LGT. In contrast, the Rhodanobacter pangenome contained a recombinational hot spot in which numerous metal resistance genes were subjected to LGT and/or duplication. In particular, Co 2+ /Zn 2+ /Cd 2+ efflux and mercuric resistance operon genes appeared to be highly mobile within Rhodanobacter populations. Evidence of multiple duplications of a mercuric resistance operon common to most Rhodanobacter strains was also observed. Collectively, our analyses indicated the importance of LGT during the evolution of groundwater microbial communities in response to heavy metal contamination, and a conceptual model was developed to display such adaptive evolutionary processes for explaining the extreme dominance of Rhodanobacter populations in the contaminated groundwater microbiome. IMPORTANCE Lateral gene transfer (LGT), along with positive selection and gene duplication, are the three main mechanisms that drive adaptive evolution of microbial genomes and communities, but their relative importance is unclear. Some recent studies suggested that LGT is a major adaptive mechanism for microbial populations in response to changing environments, and hence, it could also be critical in shaping microbial community structure. However, direct evidence of LGT and its rates in extant natural microbial communities in response to changing environments is still lacking. Our results presented in this study provide explicit evidence that LGT played a crucial role in driving the evolution of a groundwater microbial community in response to extreme heavy metal contamination. It appears that acquisition of genes critical for survival, growth, and reproduction via LGT is the most rapid and effective way to enable microorganisms and associated microbial communities to quickly adapt to abrupt harsh environmental stresses.

Biotransformation of cis-1,2-dichloroethylene to ethylene and ethane under anaerobic conditions

1994 ◽  
Vol 30 (7) ◽  
pp. 75-84 ◽  
Author(s):  
T. Komatsu ◽  
K. Momonoi ◽  
T. Matsuo ◽  
K. Hanaki
Keyword(s):  
Yeast Extract ◽  
Reductive Dechlorination ◽  
Organic Substrate ◽  
Contaminated Groundwater ◽  
Transformation Rate ◽  
Anaerobic Conditions ◽  
Factors Affecting ◽  
Low Concentrations ◽  
Anaerobic Bioremediation ◽  
Bioremediation Processes

cis-1,2-Dichloroethylene (cis-DCE) is frequently found at significant concentrations in groundwater which is contaminated with tetrachloroethylene or trichloroethylene. Under anaerobic conditions, cis-DCE can be biotransformed via reductive dechlorination to ethylene. Several factors affecting this transformation were investigated using anaerobic sewage sludge as an inoculum. The reductive dechlorination of cis-DCE was observed at 25°C and 15°C but not at 35°C. Supplying a suitable electron donor (organic substrate or hydrogen) was necessary to sustain reductive dechlorination. Glucose, yeast extract, propionate, and hydrogen stimulated dechlorination, while methanol and acetate did not. Anaerobic enrichment cultures capable of dechlorinating cis-DCE to ethylene were developed from the sludge. In the presence of either glucose, yeast extract or propionate (100 mgCOD/l), 0.46 mg/l of cis-DCE was almost completely dechlorinated to ethylene within 4 days by the cultures at 25°C. Transformation rate was somewhat lower in the culture fed with hydrogen. Dechlorinating ability was sustained even in the cultures fed with low concentrations (10 mgCOD/l) of glucose or hydrogen, although the transformation was sometimes insufficient. These results suggest that anaerobic bioremediation processes can be used for removal of chlorinated ethylenes from contaminated groundwater.

scholarly journals Functional Gene Array-Based Ultrasensitive and Quantitative Detection of Microbial Populations in Complex Communities

mSystems ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Zhou Shi ◽  
Huaqun Yin ◽  
Joy D. Van Nostrand ◽  
James W. Voordeckers ◽  
Qichao Tu ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Growth Promotion ◽  
Rapid Development ◽  
Environmental Contaminants ◽  
Gene Array ◽  
Functional Gene ◽  
Ecosystem Functions ◽  
Quantitative Detection ◽  
Contaminated Groundwater ◽  
Microbial Systems

ABSTRACTWhile functional gene arrays (FGAs) have greatly expanded our understanding of complex microbial systems, specificity, sensitivity, and quantitation challenges remain. We developed a new generation of FGA, GeoChip 5.0, using the Agilent platform. Two formats were created, a smaller format (GeoChip 5.0S), primarily covering carbon-, nitrogen-, sulfur-, and phosphorus-cycling genes and others providing ecological services, and a larger format (GeoChip 5.0M) containing the functional categories involved in biogeochemical cycling of C, N, S, and P and various metals, stress response, microbial defense, electron transport, plant growth promotion, virulence,gyrB, and fungus-, protozoan-, and virus-specific genes. GeoChip 5.0M contains 161,961 oligonucleotide probes covering >365,000 genes of 1,447 gene families from broad, functionally divergent taxonomic groups, including bacteria (2,721 genera), archaea (101 genera), fungi (297 genera), protists (219 genera), and viruses (167 genera), mainly phages. Computational and experimental evaluation indicated that designed probes were highly specific and could detect as little as 0.05 ng of pure culture DNAs within a background of 1 μg community DNA (equivalent to 0.005% of the population). Additionally, strong quantitative linear relationships were observed between signal intensity and amount of pure genomic (∼99% of probes detected;r> 0.9) or soil (∼97%;r> 0.9) DNAs. Application of the GeoChip to a contaminated groundwater microbial community indicated that environmental contaminants (primarily heavy metals) had significant impacts on the biodiversity of the communities. This is the most comprehensive FGA to date, capable of directly linking microbial genes/populations to ecosystem functions.IMPORTANCEThe rapid development of metagenomic technologies, including microarrays, over the past decade has greatly expanded our understanding of complex microbial systems. However, because of the ever-expanding number of novel microbial sequences discovered each year, developing a microarray that is representative of real microbial communities, is specific and sensitive, and provides quantitative information remains a challenge. The newly developed GeoChip 5.0 is the most comprehensive microarray available to date for examining the functional capabilities of microbial communities important to biogeochemistry, ecology, environmental sciences, and human health. The GeoChip 5 is highly specific, sensitive, and quantitative based on both computational and experimental assays. Use of the array on a contaminated groundwater sample provided novel insights on the impacts of environmental contaminants on groundwater microbial communities.

Enhanced In-Situ Anaerobic Bioremediation of TCE-Contaminated Groundwater Using Nanoscale Zero-Valent Iron (nZVI)

2011 ◽  
Vol 694 ◽  
pp. 3-7 ◽  
Author(s):  
K.F. Chen ◽  
C.M. Kao ◽  
W.P. Sung ◽  
C.C. Lin ◽  
T.Y. Yeh
Keyword(s):  
Hydrogen Production ◽  
Reduction Process ◽  
Field Application ◽  
Zero Valent Iron ◽  
Contaminated Site ◽  
Contaminated Groundwater ◽  
Good Efficiency ◽  
Nanoscale Zero Valent Iron ◽  
Anaerobic Bioremediation

In this study, a trichloroethylene (TCE)-spill site was selected to assess the feasibility of enhanced in situ anaerobic bioremediation of TCE-contaminated groundwater using hydrogen as the electron donor produced from nanoscale zero-valent iron (nZVI). Results of hydrogen production experiments show that nZVI had a good efficiency on hydrogen production. Results of microcosm study show that indigenous microorganisms were capable of degrading TCE under anaerobic reductive dechlorinating conditions. Compared to the live control and autoclaved control microcosms, microcosms with hydrogen addition significantly enhanced the TCE removal rates. Results imply that nZVI can be applied as the source of hydrogen to bioremediate TCE-contaminated groundwater under anaerobic conditions. Except for the biotic mechanism, the supplied nZVI can also cause the TCE degradation via abiotic mechanism through oxidation-reduction process. For field application, if proper doses of iron nanoparticles can be applied in the mid- or downgradient areas, both chemical and biological mechanisms can enhance the removal of the contaminants and their byproducts. Knowledge and comprehension obtained in this study will be helpful in designing an enhanced in situ anaerobic bioremediation system for a TCE-contaminated site. The nZVI treatment scheme would be expected to provide a more cost-effective alternative to remediate chlorinated-solvent contaminated aquifers.

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