scholarly journals Tissue Expression Analysis and Characterization of Smad3 Promoter in Bovine Myoblasts and Preadipocytes

2018 ◽  
Vol 37 (6) ◽  
pp. 551-559 ◽  
Author(s):  
Le Zhang ◽  
Yue Ning ◽  
Peiwei Li ◽  
Hongfang Guo ◽  
Linsen Zan
Keyword(s):  
Expression Analysis ◽  
Tissue Expression ◽  
Tissue Expression Analysis

Tissue expression analysis, cloning and characterization of the 5′-regulatory region of the bovine FABP3 gene

2016 ◽  
Vol 43 (9) ◽  
pp. 991-998 ◽  
Author(s):  
Anning Li ◽  
Lijuan Wu ◽  
Xiaoyu Wang ◽  
Yaping Xin ◽  
Linsen Zan
Keyword(s):  
Expression Analysis ◽  
Regulatory Region ◽  
Tissue Expression ◽  
Tissue Expression Analysis

Molecular cloning, sequence characteristics, and tissue expression analysis of ECE1 gene in Tibetan pig

Gene ◽  
2015 ◽  
Vol 571 (2) ◽  
pp. 237-244 ◽  
Author(s):  
Yan-dong Wang ◽  
Jian Zhang ◽  
Chuan-hao Li ◽  
Hai-peng Xu ◽  
Wei Chen ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Expression Analysis ◽  
Tissue Expression ◽  
Tibetan Pig ◽  
Tissue Expression Analysis ◽  
Sequence Characteristics

scholarly journals Identification and Expression Analysis of Stress-Associated Proteins (SAPs) Containing A20/AN1 Zinc Finger in Cucumber

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 400
Author(s):  
Wei Lai ◽  
Yong Zhou ◽  
Rao Pan ◽  
Liting Liao ◽  
Juncheng He ◽  
...  
Keyword(s):  
Gene Family ◽  
Zinc Finger ◽  
Expression Analysis ◽  
Stress Responses ◽  
Tissue Expression ◽  
Future Research ◽  
Transcriptional Responses ◽  
Cucumis Sativus L ◽  
Tissue Expression Analysis ◽  
Associated Proteins

Stress-associated proteins (SAPs) are a class of zinc finger proteins that confer tolerance to a variety of abiotic and biotic stresses in diverse plant species. However, in cucumber (Cucumis sativus L.), very little is known about the roles of SAP gene family members in regulating plant growth, development, and stress responses. In this study, a total of 12 SAP genes (named as CsSAP1-CsSAP12) were identified in the cucumber genome, which were unevenly distributed on six chromosomes. Gene duplication analysis detected one tandem duplication and two segmental duplication events. Phylogenetic analysis of SAP proteins from cucumber and other plants suggested that they could be divided into seven groups (sub-families), and proteins in the same group generally had the same arrangement of AN1 (ZnF-AN1) and A20 (ZnF-A20) domains. Most of the CsSAP genes were intronless and harbored a number of stress- and hormone-responsive cis-elements in their promoter regions. Tissue expression analysis showed that the CsSAP genes had a broad spectrum of expression in different tissues, and some of them displayed remarkable alteration in expression during fruit development. RT-qPCR results indicated that all the selected CsSAP genes displayed transcriptional responses to cold, drought, and salt stresses. These results enable the first comprehensive description of the SAP gene family in cucumber and lay a solid foundation for future research on the biological functions of CsSAP genes.

scholarly journals An Interscholastic Network to Generate LexA Enhancer Trap Lines in Drosophila

2019 ◽  
Author(s):  
Lutz Kockel ◽  
Catherine Griffin ◽  
Yaseen Ahmed ◽  
Lauren Fidelak ◽  
Arjun Rajan ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Tissue Expression ◽  
Enhancer Trap ◽  
Source Distribution ◽  
Expression Systems ◽  
Tissue Expression Analysis ◽  
Experimental Tool ◽  
Chromosome Iii ◽  
Enhancer Trap Lines

AbstractBinary expression systems like the LexA-LexAop system provide a powerful experimental tool kit to study gene and tissue function in developmental biology, neurobiology and physiology. However, the number of well-defined LexA enhancer trap insertions remains limited. In this study, we present the molecular characterization and initial tissue expression analysis of nearly 100 novel StanEx LexA enhancer traps, derived from the StonEx1 index line. This includes 76 insertions into novel, distinct gene loci not previously associated with enhancer traps or targeted LexA constructs. Additionally, our studies revealed evidence for selective transposase-dependent replacement of a previously-undetected KP element on chromosome III within the StanEx1 genetic background during hybrid dysgenesis, suggesting a molecular basis for the over-representation of LexA insertions at the NK7.1 locus in our screen. Production and characterization of novel fly lines were performed by students and teachers in experiment-based genetics classes within a geographically diverse network of public and independent high schools. Thus, unique partnerships between secondary schools and university-based programs have produced and characterized novel genetic and molecular resources in Drosophila for open-source distribution, and provide paradigms for development of science education through experience-based pedagogy.

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