Molecular Characterization, Expression Patterns, and Polymorphism of a Differentially Expressed Porcine Gene (PYGM) Isolated by Suppression Subtractive Hybridization and Two-Dimensional Gel Electrophoresis Analysis

2012 ◽  
Vol 31 (1) ◽  
pp. 98-105
Author(s):  
Yongjie Xu ◽  
Wenmin Yu ◽  
Xiaoting Feng ◽  
Hongtao Xie ◽  
Yuanzhu Xiong
Keyword(s):  
Molecular Characterization ◽  
Suppression Subtractive Hybridization ◽  
Gel Electrophoresis ◽  
Expression Patterns ◽  
Subtractive Hybridization ◽  
Differentially Expressed ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Porcine Gene

scholarly journals Proteomic identification of glutamine synthetase as a differential marker for oligodendrogliomas and astrocytomas

2011 ◽  
Vol 115 (4) ◽  
pp. 789-795 ◽  
Author(s):  
Zhengping Zhuang ◽  
Meng Qi ◽  
Jie Li ◽  
Hiroaki Okamoto ◽  
David S. Xu ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Glial Cell ◽  
Cell Cultures ◽  
Gel Electrophoresis ◽  
Expression Patterns ◽  
Morphological Variability ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Two Dimensional Gel Electrophoresis ◽  
Glial Cell Cultures

Object Astrocytomas and oligodendrogliomas are primary CNS tumors that remain a challenge to differentiate histologically because of their morphological variability and because there is a lack of reliable differential diagnostic markers. To identify proteins that are differentially expressed between astrocytomas and oligodendrogliomas, the authors analyzed the proteomic expression patterns and identified uniquely expressed proteins in these neoplasms. Methods Proteomes of astrocytomas and oligodendrogliomas were analyzed using 2D gel electrophoresis and subsequent computerized gel analysis to detect differentially expressed proteins. The proteins were identified using high-performance liquid chromatography accompanied by tandem mass spectrometry. To determine the role of the differentially expressed proteins in astrocytes, undifferentiated glial cell cultures were treated with dibutyryl–cyclic adenosine monophosphate (cAMP). Results Two-dimensional gel electrophoresis revealed that glutamine synthetase was differentially expressed in astrocytomas and oligodendrogliomas. Western blot and immunohistochemical analyses confirmed the increased expression of glutamine synthetase in astrocytomas compared with oligodendrogliomas. Whereas glutamine synthetase expression was demonstrated across all grades of astrocytomas (Grade II–IV [15 tumors]) and oligoastrocytomas (4 tumors), it was expressed in only 1 oligodendroglioma (6% [16 tumors]). Treatment of undifferentiated glial cell cultures with dibutyryl-cAMP resulted in astrocyte differentiation that was associated with increased levels of glial fibrillary acidic protein and glutamine synthetase. Conclusions These data indicate that glutamine synthetase expression can be used to distinguish astrocytic from oligodendroglial tumors and may play a role in the pathogenesis of astrocytomas.

Global approaches to quantitative analysis of gene-expression patterns observed by use of two-dimensional gel electrophoresis.

1984 ◽  
Vol 30 (12) ◽  
pp. 2031-2036 ◽  
Author(s):  
N L Anderson ◽  
J P Hofmann ◽  
A Gemmell ◽  
J Taylor
Keyword(s):  
Gel Electrophoresis ◽  
Quantitative Data ◽  
Preliminary Analysis ◽  
Expression Patterns ◽  
Principal Component ◽  
Cell Types ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Analysis Techniques ◽  
And Cluster Analysis

Abstract A major difficulty in the use of two-dimensional protein maps to identify and classify cell types is the problem of acquiring, selecting, and analyzing quantitative data on hundreds of protein spots. Here we use methods of multivariate statistics to analyze the differences among a panel of human cell lines, in some cases involving quantitative data on more than 250 proteins. Principal-component and cluster-analysis techniques show that the lines can be easily distinguished, even by using the subset of proteins present in all cells. A preliminary analysis of the protein changes brought about by phorbol ester-induced differentiation of the line U937 is included.

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

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