Transcriptional Status of Known and Novel Genes Tagged with Consensus of 33.15 Repeat Loci Employing Minisatellite-Associated Sequence Amplification (MASA) and Real-Time PCR in Water Buffalo, Bubalus bubalis

2006 ◽  
Vol 25 (1) ◽  
pp. 31-48 ◽  
Author(s):  
Jyoti Srivastava ◽  
Sanjay Premi ◽  
Deepali Pathak ◽  
Zaid Ahsan ◽  
Madhulika Tiwari ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Novel Genes

Development and Evaluation of Real-Time PCR Assay for the Detection of Babesia orientalis In Water Buffalo (Bubalus bubalis, Linnaeus, 1758)

2011 ◽  
Vol 97 (6) ◽  
pp. 1166-1169 ◽  
Author(s):  
Lan He ◽  
Hui-Hui Feng ◽  
Qin-Li Zhang ◽  
Wen-Jie Zhang ◽  
Muhanmad Kasib Khan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Pcr Assay ◽  
Babesia Orientalis

Using real-time PCR to identify pregnancy-associated glycoprotein 2 (PAG-2) in water buffalo ( Bubalus bubalis ) blood in early pregnancy

2017 ◽  
Vol 89 ◽  
pp. 106-113
Author(s):  
O. Barbato ◽  
G. Guelfi ◽  
V.L. Barile ◽  
L. Menchetti ◽  
C. Tortiello ◽  
...  
Keyword(s):  
Real Time ◽  
Early Pregnancy ◽  
Real Time Pcr ◽  
Water Buffalo ◽  
Bubalus Bubalis

Detection of Cow Milk in Water Buffalo Cheese by SYBR Green Real-Time PCR: Sensitivity Test on Governing Liquid Samples

2006 ◽  
Vol 6 (1) ◽  
pp. 94-98 ◽  
Author(s):  
Maria Feligini . ◽  
Naima Alim . ◽  
Ivan Bonizzi . ◽  
Giuseppe Enne . ◽  
Riccardo Aleandri .
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Water Buffalo ◽  
Sybr Green ◽  
Sensitivity Test ◽  
Cow Milk ◽  
Liquid Samples

Identification of differentially expressed genes in response to dietary iron deprivation in rat duodenum

2005 ◽  
Vol 288 (5) ◽  
pp. G964-G971 ◽  
Author(s):  
James F. Collins ◽  
Christina A. Franck ◽  
Kris V. Kowdley ◽  
Fayez K. Ghishan
Keyword(s):  
Iron Deficiency ◽  
Real Time ◽  
Real Time Pcr ◽  
Iron Transport ◽  
Dietary Iron ◽  
Cancer Resistance ◽  
Novel Genes ◽  
Copper Absorption ◽  
Iron Deprivation ◽  
Iron Deficient

We sought to identify novel genes involved in intestinal iron absorption by inducing iron deficiency in rats during postnatal development from the suckling period through adulthood. We then performed comparative gene chip analyses (RAE230A and RAE230B chips; Affymetrix) with cRNA derived from duodenal mucosa. Real-time PCR was used to confirm changes in gene expression. Genes encoding the apical iron transport-related proteins [ divalent metal transporter 1 (DMT1) and duodenal cytochrome b] were strongly induced at all ages studied, whereas increases in mRNA encoding the basolateral proteins iron-regulated gene 1 and hephaestin were observed only by real-time PCR. In addition, transferrin receptor 1 and heme oxygenase 1 were induced. We also identified induction of novel genes not previously associated with intestinal iron transport. The Menkes copper ATPase (ATP7a) and metallothionein were strongly induced at all ages studied, suggesting increased copper absorption by enterocytes during iron deficiency. We also found significantly increased liver copper levels in 7- to 12-wk-old iron-deficient rats. Also upregulated at most ages examined were the sodium-dependent vitamin C transporter, tripartite motif protein 27, aquaporin 4, lipocalin-interacting membrane receptor, and the breast cancer-resistance protein (ABCG2). Some genes also showed decreased expression with iron deprivation, including several membrane transporters, metabolic enzymes, and genes involved in the oxidative stress response. We speculate that dietary iron deprivation leads to increased intestinal copper absorption via DMT1 on the brush-border membrane and the Menkes copper ATPase on the basolateral membrane. These findings may thus explain copper loading in the iron-deficient state. We also demonstrate that many other novel genes may be differentially regulated in the setting of iron deprivation.

Validation of a Real-time PCR assay for fast and sensitive quantification of Brucella spp. in water buffalo milk

Food Control ◽  
2011 ◽  
Vol 22 (8) ◽  
pp. 1466-1470 ◽  
Author(s):  
Maria Grazia Amoroso ◽  
Caterina Salzano ◽  
Barbara Cioffi ◽  
Michele Napoletano ◽  
Francesca Garofalo ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Water Buffalo ◽  
Buffalo Milk ◽  
Pcr Assay

scholarly journals Isolation, bioinformatic analysis and tissue expression profile of a novel water buffalo gene-<i>MFG-E8</i>

2013 ◽  
Vol 56 (1) ◽  
pp. 833-841
Author(s):  
C. Wu ◽  
L. Liu ◽  
J. Huo ◽  
D. Li ◽  
Y. Yuan ◽  
...  
Keyword(s):  
Amino Acids ◽  
Mammary Gland ◽  
Real Time ◽  
Expression Profile ◽  
Real Time Pcr ◽  
Water Buffalo ◽  
Critical Role ◽  
Tissue Expression ◽  
Coding Region ◽  
Tissue Expression Profile

Abstract. Milk fat globule-epidermal growth factor 8 (MFG-E8) is a milk membrane-associated glycoprotein, which plays a critical role in phagocytic clearance of apoptotic cells and mammary gland development, involution and remodeling. In the present study, the complete CDS of water buffalo MFG-E8 was obtained and characterized. The genetic variations of nine water buffalo were investigated. Further, the tissue expression profile was carried out using Quantitative real-time PCR method. The full-length coding region of MFG-E8 from water buffalo tissues consists of 1296 nucleotides, which encodes 431 amino acids with a molecular weight of 47.85 kDa and a pI of 7.02. Bioinformatic prediction indicates that the MFG-E8 protein contains one signal peptide, two repeated EGF-like domains in its N-terminal side and two repeated discoidinlike Factor 5/Factor 8 domains in its amino acids. The sequence homology analysis in Bovidae family revealed that the coding region of water buffalo MFG-E8 had 98.5, 98.3, 93.9, and 88.0 % identity with that of cattle, yak, goat and sheep. There was no polymorphism found in water buffalo, but there existed five nucleotide differences between water buffalo and other bovine species. The phylogenetic tree based on the amino acid sequences of MFG-E8 from thirteen species revealed that buffalo had a closer genetic relationship with the species of Bovidae family. Real-time PCR analysis showed that water buffalo MFG-E8 gene was expressed in various tissues, but at different levels. The expression levels of this gene were higher in the mammary gland than in other tissues, suggesting that the MFG-E8 protein plays a role in mammary gland functions.

Feldvalidierung einer real-time PCR zum Nachweis von Mycoplasma hyopneumoniae in Nasentupfermaterial von lebenden Schweinen

2005 ◽  
Vol 147 (9) ◽  
pp. 373-379 ◽  
Author(s):  
F. Zeeh ◽  
P. Kuhnert ◽  
R. Miserez ◽  
M. G. Doherr ◽  
W. Zimmermann
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mycoplasma Hyopneumoniae
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