Lactase Gene Promoter Fragments Mediate Differential Spatial and Temporal Expression Patterns in Transgenic Mice

2006 ◽  
Vol 25 (4) ◽  
pp. 215-222 ◽  
Author(s):  
Zhi Wang ◽  
Charalambos Maravelias ◽  
Eric Sibley
Keyword(s):  
Transgenic Mice ◽  
Expression Patterns ◽  
Gene Promoter ◽  
Temporal Expression ◽  
Lactase Gene

Lactase promoter fragments direct differential in vivo spatio-temporal expression patterns in transgenic mice

2000 ◽  
Vol 118 (4) ◽  
pp. A291
Author(s):  
So Young Lee ◽  
Chun-ku Lin ◽  
Christopher H. Contag ◽  
Allen D. Cooper ◽  
Eric Sibley
Keyword(s):  
Transgenic Mice ◽  
Expression Patterns ◽  
Temporal Expression ◽  
Spatio Temporal

BMP signaling is essential for development of skeletogenic and neurogenic cranial neural crest

Development ◽  
2000 ◽  
Vol 127 (5) ◽  
pp. 1095-1104 ◽  
Author(s):  
B. Kanzler ◽  
R.K. Foreman ◽  
P.A. Labosky ◽  
M. Mallo
Keyword(s):  
Transgenic Mice ◽  
Neural Crest ◽  
Essential Role ◽  
Expression Patterns ◽  
Neural Crest Cells ◽  
Bmp Signaling ◽  
Cranial Neural Crest ◽  
Temporal Expression ◽  
Branchial Arches

BMP signaling is essential for a wide variety of developmental processes. To evaluate the role of Bmp2/4 in cranial neural crest (CNC) formation or differentiation after its migration into the branchial arches, we used Xnoggin to block their activities in specific areas of the CNC in transgenic mice. This resulted in depletion of CNC cells from the targeted areas. As a consequence, the branchial arches normally populated by the affected neural crest cells were hypomorphic and their skeletal and neural derivatives failed to develop. In further analyses, we have identified Bmp2 as the factor required for production of migratory cranial neural crest. Its spatial and temporal expression patterns mirror CNC emergence and Bmp2 mutant embryos lack both branchial arches and detectable migratory CNC cells. Our results provide functional evidence for an essential role of BMP signaling in CNC development.

scholarly journals Tissue-specific regulation of the alpha-myosin heavy chain gene promoter in transgenic mice.

1991 ◽  
Vol 266 (36) ◽  
pp. 24613-24620
Author(s):  
A. Subramaniam ◽  
W.K. Jones ◽  
J. Gulick ◽  
S. Wert ◽  
J. Neumann ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Gene Promoter ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Myosin Heavy Chain Gene ◽  
Tissue Specific ◽  
Specific Regulation

Expression patterns of Wnts, Frizzleds, sFRPs, and misexpression in transgenic mice suggesting a role for Wnts in pancreas and foregut pattern formation

2002 ◽  
Vol 225 (3) ◽  
pp. 260-270 ◽  
Author(s):  
R. Scott Heller ◽  
Darwin S. Dichmann ◽  
Jan Jensen ◽  
Chris Miller ◽  
Gordon Wong ◽  
...  
Keyword(s):  
Pattern Formation ◽  
Transgenic Mice ◽  
Expression Patterns

MULTIPLE PAR AND E4BP4 bZIP TRANSCRIPTION FACTORS IN ZEBRAFISH: DIVERSE SPATIAL AND TEMPORAL EXPRESSION PATTERNS

2010 ◽  
Vol 27 (8) ◽  
pp. 1509-1531 ◽  
Author(s):  
Zohar Ben-Moshe ◽  
Gad Vatine ◽  
Shahar Alon ◽  
Adi Tovin ◽  
Philipp Mracek ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Expression Patterns ◽  
Temporal Expression ◽  
Bzip Transcription Factors

Abstract 1414: Pim-1 Stimulates Cardiac Progenitor Cell Proliferation And Asymmetric Division

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Christopher T Cottage ◽  
Savilla Tuck ◽  
Kimberlee Fischer ◽  
Natalie Gude ◽  
John Muraski ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cell Fate ◽  
Cellular Proliferation ◽  
Gene Promoter ◽  
Asymmetric Division ◽  
Postnatal Growth ◽  
Chain Gene ◽  
Ki 67 ◽  
Population Number ◽  
Mechanistic Basis

Cardiac progenitor cells (CPCs) blunt cardiomyopathic damage and increase survival following adoptive transfer into hearts subjected to myocardial infarction (MI), but the initial survival, persistence, and long term engraftment of the donated cell population remains problematic. Previous studies from our group have demonstrated that transgenes driven by the α -myosin heavy chain gene promoter are expressed in the CPC population allowing for enhanced proliferation and survival. This study details a genetic engineering strategy to augment the salutary effects of CPCs through the use of a serine/threonine kinase named Pim-1 that promotes cellular proliferation and survival. Transgenic mice created with cardiac-specific Pim-1 overexpression (Pim-wt) exhibit enhanced Pim-1 activity in both cardiomyocytes and CPCs, both of which show increased proliferative activity assessed using BrdU or Ki-67 markers relative to non-transgenic (NTG) controls. However, CPC population number was not increased in the Pim-wt hearts during normal postnatal growth or after infarction challenge, suggesting that Pim-1 expression promotes asymmetric division resulting in maintenance of the CPC pool as well as expansion of the cardiomyocyte population. Localization and quantitation of cell fate determinants Numb and α -adaptin by confocal microscopy were employed to assess levels of asymmetric division in the CPC population. Polarization of Numb in mitotic phospho-histone positive cells demonstrates asymmetric division in 65% of the CPC population in hearts of Pim-wt mice versus 26% in NTG hearts after infarction challenge. Similarly, Pim-wt hearts had fewer cells with uniform α -adaptin staining indicative of symmetrically dividing CPCs, with in 36% of the CPCs versus vs. 73% in NTG sections. These findings define a mechanistic basis for enhanced myocardial regeneration in transgenic mice overexpressing Pim-1 kinase in the myocardial lineage cells.

Reduction of dietary obesity in aP2-Ucp transgenic mice: physiology and adipose tissue distribution

1996 ◽  
Vol 270 (5) ◽  
pp. E768-E775 ◽  
Author(s):  
J. Kopecky ◽  
Z. Hodny ◽  
M. Rossmeisl ◽  
I. Syrovy ◽  
L. P. Kozak
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Transgenic Mice ◽  
Body Weight Gain ◽  
Uncoupling Protein ◽  
Gene Promoter ◽  
Lipid Binding ◽  
Brown Fat ◽  
Mitochondrial Uncoupling ◽  
Dietary Obesity

We seek to determine whether increased energy dissipation in adipose tissue can prevent obesity. Transgenic mice with C57BL6/J background and the adipocyte lipid-binding protein (aP2) gene promoter directing expression of the mitochondrial uncoupling protein (UCP) gene in white and brown fat were used. Physiologically, UCP is essential for nonshivering thermogenesis in brown fat. Mice were assigned to a chow or a high-fat (HF) diet at 3 mo of age. Over the next 25 wk, gains of body weight were similar in corresponding subgroups (n = 6-8) of female and male mice: 4-5 g in chow nontransgenic and transgenic, 20 g in HF nontransgenic, and 9-11 g in HF transgenic mice. The lower body weight gain in the HF transgenic vs. nontransgenic mice corresponded to a twofold lower feed efficiency. Gonadal fat was enlarged, but subcutaneous white fat was decreased in the transgenic vs. nontransgenic mice in both dietary conditions. The results suggest that UCP synthesized from the aP2 gene promoter is capable of reducing dietary obesity.

scholarly journals Cloning, Characterization, and Expression Features of Chicken CDS2 Splicing Variants

2020 ◽  
Author(s):  
Yuanyuan Xu ◽  
Shuping Zhang ◽  
Yujun Guo ◽  
Wen Chen ◽  
Yanqun Huang
Keyword(s):  
Adipose Tissue ◽  
Real Time ◽  
Real Time Pcr ◽  
Expression Patterns ◽  
Mrna Levels ◽  
Exogenous Insulin ◽  
Quantitative Real Time Pcr ◽  
Temporal Expression ◽  
Spatio Temporal ◽  
The Brain

Abstract Background: The CDS gene encodes the CDP-diacylglycerol synthase enzyme that catalyzes the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid. At present, there are no reports of CDS2 in birds. Here, we identified chicken CDS2 transcripts by combining conventional RT- PCR amplification, 5' RACE (Fig. 1A), and 3' RACE, explored the spatio-temporal expression profiles of total CDS2 and the longest transcript variant CDS2-4, and investigated the effect of exogenous insulin on total the mRNA level of CDS2 by quantitative real-time PCR. Results: Four transcripts of chicken CDS2 (CDS2-1, -2, -3, and -4) were identified, which were alternatively spliced at the 3′-untranslated region (UTR). CDS2 was widely expressed in all tissues examined and the longest variant CDS2-4 was the major transcript. Both total CDS2 and CDS2-4 were prominently expressed in adipose tissue and the heart, and exhibited low expression in the liver and pectoralis of 49 day-old chickens. Quantitative real-time PCR revealed that total CDS2 and CDS2-4 had different spatio-temporal expression patterns in chicken. Total CDS2 exhibited a similar temporal expression tendency with a high level in the later period of incubation (embryonic day 19 [E19] or 1-day-old) in the brain, liver, and pectoralis. While CDS2-4 presented a distinct temporal expression pattern in these tissues, CDS2-4 levels peaked at 21 days in the brain and pectoralis, while liver CDS2-4 mRNA levels were highest at the early stage of hatching (E10). Total CDS2 (P < 0.001) and CDS2-4 (P = 0.0090) mRNA levels in the liver were differentially regulated throughout development of the chicken. Exogenous insulin significantly downregulated the level of total CDS2 at 240 min in the pectoralis of Silky chickens (P < 0.01). Total CDS2 levels in the liver of Silky chickens were higher than that of the broiler in the basal state and after insulin stimulation. Conclusion: Chicken CDS2 has multiple transcripts with variation at the 3′-UTR, which was prominently expressed in adipose tissue. Total CDS2 and CDS2-4 presented distinct spatio-temporal expression patterns, and they were differentially regulated with age in liver. Insulin could regulate chicken CDS2 levels in a breed- and tissue-specific manner.

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