Characterization of SHATTERPROOF Homoeologs and CRISPR-Cas9-Mediated Genome Editing Enhances Pod-Shattering Resistance in Brassica napus L.

2021 ◽  
Vol 4 (3) ◽  
pp. 360-370
Author(s):  
Qamar U. Zaman ◽  
Chu Wen ◽  
Shi Yuqin ◽  
Hao Mengyu ◽  
Mei Desheng ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genome Editing ◽  
Brassica Napus L ◽  
Pod Shattering

scholarly journals CRISPR/Cas9-Mediated Multiplex Genome Editing of JAGGED Gene in Brassica napus L.

Biomolecules ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 725 ◽  
Author(s):  
Qamar U Zaman ◽  
Wen Chu ◽  
Mengyu Hao ◽  
Yuqin Shi ◽  
Mengdan Sun ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genome Editing ◽  
Gene Knockout ◽  
Cylindrical Body ◽  
High Yield ◽  
Wild Type ◽  
Brassica Napus L ◽  
Pod Shattering ◽  
Undifferentiated Cells ◽  
Key Factor

Pod shattering resistance is an essential component to achieving a high yield, which is a substantial objective in polyploid rapeseed cultivation. Previous studies have suggested that the Arabidopsis JAGGED (JAG) gene is a key factor implicated in the regulatory web of dehiscence fruit. However, its role in controlling pod shattering resistance in oilseed rape is still unknown. In this study, multiplex genome editing was carried out by the CRISPR/Cas9 system on five homoeologs (BnJAG.A02, BnJAG.C02, BnJAG.C06, BnJAG.A07, and BnJAG.A08) of the JAG gene. Knockout mutagenesis of all homoeologs drastically affected the development of the lateral organs in organizing pod shape and size. The cylindrical body of the pod comprised a number of undifferentiated cells like a callus, without distinctive valves, replum, septum, and valve margins. Pseudoseeds were produced, which were divided into two halves with an incomplete layer of cells (probably septum) that separated the undifferentiated cells. These mutants were not capable of generating any productive seeds for further generations. However, one mutant line was identified in which only a BnJAG.A08-NUB-Like paralog of the JAG gene was mutated. Knockout mutagenesis in BnJAG.A08-NUB gene caused significant changes in the pod dehiscence zone. The replum region of the mutant was increased to a great extent, resulting in enlarged cell size, bumpy fruit, and reduced length compared with the wild type. A higher replum–valve joint area may have increased the resistance to pod shattering by ~2-fold in JAG mutants compared with wild type. Our results offer a basis for understanding variations in Brassica napus fruit by mutating JAG genes and providing a way forward for other Brassicaceae species.

Vernalization response in spring oilseed rape (Brassica napus L.) cultivars

1994 ◽  
Vol 74 (2) ◽  
pp. 275-277 ◽  
Author(s):  
L. A. Murphy ◽  
R. Scarth
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Vernalization Response ◽  
Brassica Napus L ◽  
Early Maturity ◽  
Breeding Programs ◽  
Cumulative Response ◽  
Cold Requirement ◽  
Key Words:Brassica

Early maturity is a major objective of oilseed rape (Brassica napus L.) breeding programs in western Canada. Maturity of crops is influenced by time of initiation and flowering. The presence of a vernalization requirement affects plant development by delaying floral initiation until the cold requirement of the plant has been satisfied. Five spring oilseed rape cultivars were screened for their response to vernalization. Vernalization treatments consisted of exposure of germinated seeds to 0–42 d at 4 °C. Plants were assessed under a 20-h photoperiod. In general, there was a cumulative response to vernalization, with a decrease in days to each developmental stage as exposure to 4 °C was increased. Vernalization treatment of 6 d at 4 °C was sufficient to decrease both the days to first flower and the final leaf number. The characterization of vernalization response is of interest because variation in flowering time in response to year-to-year variations in the environment could result. Key words:Brassica napus, canola, oilseed rape, vernalization

CRISPR/Cas9-mediated genome editing reveals differences in the contribution of INDEHISCENT homologues to pod shatter resistance in Brassica napus L.

2019 ◽  
Vol 132 (7) ◽  
pp. 2111-2123 ◽  
Author(s):  
Yungu Zhai ◽  
Shengli Cai ◽  
Limin Hu ◽  
Yang Yang ◽  
Olalekan Amoo ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genome Editing ◽  
Brassica Napus L ◽  
Pod Shatter

scholarly journals Inheritance and Molecular Characterization of a Novel Mutated AHAS Gene Responsible for the Resistance of AHAS-Inhibiting Herbicides in Rapeseed (Brassica napus L.)

2020 ◽  
Vol 21 (4) ◽  
pp. 1345
Author(s):  
Qianxin Huang ◽  
Jinyang Lv ◽  
Yanyan Sun ◽  
Hongmei Wang ◽  
Yuan Guo ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Molecular Characterization ◽  
Herbicide Resistance ◽  
Acetohydroxyacid Synthase ◽  
Cross Resistance ◽  
Single Point Mutation ◽  
Wild Type ◽  
Brassica Napus L ◽  
Herbicide Resistant

The use of herbicides is an effective and economic way to control weeds, but their availability for rapeseed is limited due to the shortage of herbicide-resistant cultivars in China. The single-point mutation in the acetohydroxyacid synthase (AHAS) gene can lead to AHAS-inhibiting herbicide resistance. In this study, the inheritance and molecular characterization of the tribenuron-methyl (TBM)-resistant rapeseed (Brassica napus L.) mutant, K5, are performed. Results indicated that TBM-resistance of K5 was controlled by one dominant allele at a single nuclear gene locus. The novel substitution of cytosine with thymine at position 544 in BnAHAS1 was identified in K5, leading to the alteration of proline with serine at position 182 in BnAHAS1. The TBM-resistance of K5 was approximately 100 times that of its wild-type ZS9, and K5 also showed cross-resistance to bensufuron-methyl and monosulfuron-ester sodium. The BnAHAS1544T transgenic Arabidopsis exhibited higher TBM-resistance than that of its wild-type, which confirmed that BnAHAS1544T was responsible for the herbicide resistance of K5. Simultaneously, an allele-specific marker was developed to quickly distinguish the heterozygous and homozygous mutated alleles BnAHAS1544T. In addition, a method for the fast screening of TBM-resistant plants at the cotyledon stage was developed. Our research identified and molecularly characterized one novel mutative AHAS allele in B. napus and laid a foundation for developing herbicide-resistant rapeseed cultivars.

scholarly journals Characterization of the BnA10.tfl1 Gene Controls Determinate Inflorescence Trait in Brassica napus L.

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 722 ◽  
Author(s):  
Yongpeng Jia ◽  
Kaixiang Li ◽  
Haidong Liu ◽  
Lingxiong Zan ◽  
Dezhi Du
Keyword(s):  
Brassica Napus ◽  
Shoot Apex ◽  
Apical Meristem ◽  
Brassica Napus L ◽  
Reading Frame ◽  
Terminal Flower ◽  
Terminal Flower 1 ◽  
Oilseed Breeding ◽  
Phosphatidylethanolamine Binding Protein

Determinate inflorescences have a significant effect on the genetic improvement of rapeseed, so understanding the molecular function underlying the inflorescence trait may be beneficial to oilseed breeding. A previous study found candidate gene BnTFL1 (Terminal Flower 1) for control of the inflorescence trait on Brassica napus chromosome A10 (16,627–16,847 kb). However, little is known about the function of the BnTFL1 gene in B. napus. In this study, we firstly studied the formation of the shoot apical meristem and gene expression in indeterminate and determinate inflorescences; the results showed that the inflorescence architecture and BnA10.TFL1 expression showed significant differences in the shoot apex at the budding stage. Then, two alleles (named BnA10.TFL1 gene from indeterminate and BnA10.tfl1 gene from determinate) were cloned and sequence-analyzed; the results suggest that the open reading frame of the alleles comprises 537 bp, encodes 178 amino acids containing a conserved phosphatidylethanolamine-binding protein (PEBP) domain, and shares high similarity with Arabidopsis thaliana TFL1. To analyze the function of BnA10.TFL1, the BnA10.TFL1 gene was introduced into the determinate A. thaliana tfl1 mutant and B. napus 571 line by complementation experiment. The determinate traits were restored to indeterminate, and expression of BnA10.TFL1 was increased in the indeterminate shoot apex. These results reveal that BnA10.tfl1 is a gene controlling the determinate inflorescence trait. Moreover, the BnA10.TFL1 protein was localized to the nucleus, cytoplasm, and plasma membrane. Collectively, the results of this study help us to understand the molecular mechanism of determinate inflorescences and will provide a reliable research basis for the application of determinate inflorescences in B. napus.

Characterization of a new temperature-sensitive male sterile line SP2S in rapeseed (Brassica napus L.)

Euphytica ◽  
2015 ◽  
Vol 206 (2) ◽  
pp. 473-485 ◽  
Author(s):  
Chengyu Yu ◽  
Yingfen Guo ◽  
Juan Ge ◽  
Yumei Hu ◽  
Jungang Dong ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Temperature Sensitive ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Brassica Napus L

Characterization of Organellar DNA in Alloplasmic Lines of Brassica napus L.

1993 ◽  
Vol 111 (3) ◽  
pp. 185-191 ◽  
Author(s):  
C. Hallden ◽  
B. Gertsson ◽  
T. Sall ◽  
C. Lind-Hallden
Keyword(s):  
Brassica Napus ◽  
Brassica Napus L ◽  
Alloplasmic Lines ◽  
Organellar Dna
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