Quantitative Analysis of Epstein-Barr Virus Supernatant by Real-Time PCR Assay

2008 ◽  
Vol 6 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Karen L. Fecenko-Tacka ◽  
Laura Schina ◽  
Christine M. Beiswanger
Keyword(s):  
Quantitative Analysis ◽  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Pcr Assay ◽  
Barr Virus ◽  
Epstein Barr

Quantitative Analysis of Epstein-Barr Virus Load by Using a Real-Time PCR Assay

1999 ◽  
Vol 37 (1) ◽  
pp. 132-136 ◽  
Author(s):  
Hiroshi Kimura ◽  
Makoto Morita ◽  
Yumi Yabuta ◽  
Kiyotaka Kuzushima ◽  
Koji Kato ◽  
...  
Keyword(s):  
Real Time ◽  
Infectious Mononucleosis ◽  
Real Time Pcr ◽  
Copy Number ◽  
Epstein Barr Virus ◽  
Pcr Assay ◽  
Virus Load ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr

To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could detect from 2 to over 107 copies of EBV DNA with a wide linear range. We estimated the virus load in peripheral blood mononuclear cells (PBMNC) from patients with symptomatic EBV infections. The mean EBV-DNA copy number in the PBMNC was 103.7 copies/μg of DNA in patients with EBV-related lymphoproliferative disorders, 104.1 copies/μg of DNA in patients with chronic active EBV infections, and 102.2copies/μg of DNA in patients with infectious mononucleosis. These numbers were significantly larger than those in either posttransplant patients or immunocompetent control patients without EBV-related diseases. In a patient with infectious mononucleosis, the virus load decreased as the symptoms resolved. The copy number of EBV DNA in PBMNC from symptomatic EBV infections was correlated with the EBV-positive cell number determined by the in situ hybridization assay (r = 0.842; P < 0.0001). These results indicate that the real-time PCR assay is useful for diagnosing symptomatic EBV infection and for monitoring the virus load.

One-step multiplex real-time PCR assay to analyse the latency patterns of Epstein-Barr virus infection

2008 ◽  
Vol 147 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Naomi Kubota ◽  
Kaoru Wada ◽  
Yoshinori Ito ◽  
Yoshie Shimoyama ◽  
Shigeo Nakamura ◽  
...  
Keyword(s):  
Virus Infection ◽  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Epstein Barr Virus Infection ◽  
Pcr Assay ◽  
Barr Virus ◽  
One Step ◽  
Epstein Barr ◽  
Barr Virus Infection

Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients

2006 ◽  
Vol 135 (2) ◽  
pp. 263-268 ◽  
Author(s):  
Sanja Kozić ◽  
Adriana Vince ◽  
Janja Iščić Beš ◽  
Oktavija Đaković Rode ◽  
Snježana Židovec Lepej ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Pcr Assay ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Comparison of Commercial Real-Time PCR Assays for Quantification of Epstein-Barr Virus DNA

2005 ◽  
Vol 43 (5) ◽  
pp. 2053-2057 ◽  
Author(s):  
G. Ruiz ◽  
P. Pena ◽  
F. de Ory ◽  
J. E. Echevarria
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Pcr Assays ◽  
Epstein Barr

Quantitation of Epstein-Barr virus mRNA using reverse transcription and real-time PCR

2004 ◽  
Vol 74 (4) ◽  
pp. 612-618 ◽  
Author(s):  
Birgit Weinberger ◽  
Annelie Plentz ◽  
Klaus M. Weinberger ◽  
Joachim Hahn ◽  
Ernst Holler ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Heterogeneous, restricted patterns of Epstein–Barr virus (EBV) latent gene expression in patients with chronic active EBV infection

2001 ◽  
Vol 82 (10) ◽  
pp. 2385-2392 ◽  
Author(s):  
Mikio Yoshioka ◽  
Nobuhisa Ishiguro ◽  
Hiroaki Ishiko ◽  
Xiaoming Ma ◽  
Hideaki Kikuta ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cell ◽  
Real Time ◽  
Real Time Pcr ◽  
Epstein Barr Virus ◽  
Clinical Symptoms ◽  
The Other ◽  
Early Gene ◽  
Barr Virus ◽  
Epstein Barr

Epstein–Barr virus (EBV) has been shown to infect T lymphocytes and to be associated with a chronic active infection (CAEBV), which has been recognized as a mainly non-neoplastic T-cell lymphoproliferative disorder (T-cell LPD). The systemic distribution of EBV genomes was studied, by real-time PCR, in multiple tissues from six patients with CAEBV, including three patients with T-cell LPD, one patient with B-cell LPD and two patients with undetermined cell-type LPD. There were extremely high loads of EBV genomes in all tissues from the patients. This reflects an abundance of circulating and infiltrating EBV-infected cells and a wide variety of clinical symptoms in the affected tissues. We chose one sample from each patient that was shown by real-time PCR to contain a high load of EBV genomes and examined the expression of EBV latent genes by RT–PCR. EBER1 and EBNA1 transcripts were detected in all samples. Only one sample also expressed EBNA2, LMP1 and LMP2A transcripts in addition to EBER1 and EBNA1 transcripts. Two of the remaining five samples expressed LMP1 and LMP2A transcripts. One sample expressed LMP2A but not LMP1 and EBNA2 transcripts. Another sample expressed EBNA2 but not LMP1 and LMP2A transcripts. The other sample did not express transcripts of any of the other EBNAs or LMPs. None of the samples expressed the viral immediate-early gene BZLF1. These results showed that EBV latent gene expression in CAEBV is heterogeneous and that restricted forms of EBV latency might play a pathogenic role in the development of CAEBV.

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