Inhibition of Mycobacterium tuberculosis tRNA-Ligases Using siRNA-Based Gene Silencing Method: A Computational Approach

2020 ◽  
Vol 27 (1) ◽  
pp. 91-99
Author(s):  
Partha Sarathi Mohanty ◽  
Sandeep Sharma ◽  
Farah Naaz ◽  
Dilip Kumar ◽  
Archana Raikwar ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Gene Silencing ◽  
Computational Approach

Elucidating the biosynthetic pathways of volatile organic compounds in Mycobacterium tuberculosis through a computational approach

2017 ◽  
Vol 13 (4) ◽  
pp. 750-755 ◽  
Author(s):  
Purva Bhatter ◽  
Karthik Raman ◽  
Vani Janakiraman
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Computational Approach ◽  
Biosynthetic Pathways ◽  
First Report ◽  
Volatile Organic

A first report on the biosynthetic origin ofMycobacterium tuberculosisspecific small volatile molecules.

Computational approach to design potential siRNA for CDKN2A gene silencing in melanoma through RNA interference

Gene Reports ◽  
2019 ◽  
Vol 17 ◽  
pp. 100469
Author(s):  
Aqsa Syed ◽  
Rabia Iftikhar ◽  
Momna Arif ◽  
Azhar Mahmood ◽  
Sajid Khan ◽  
...  
Keyword(s):  
Rna Interference ◽  
Gene Silencing ◽  
Computational Approach ◽  
Cdkn2a Gene

scholarly journals Reversible gene silencing through frameshift indels and frameshift scars provide adaptive plasticity for Mycobacterium tuberculosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Aditi Gupta ◽  
David Alland
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Gene Silencing ◽  
Population Level ◽  
Adaptive Plasticity ◽  
Linguistic Complexity ◽  
Reading Frame ◽  
Gene Position ◽  
Frame Shift ◽  
Show Evidence ◽  
Indel Frequency

AbstractMycobacterium tuberculosis can adapt to changing environments by non-heritable mechanisms. Frame-shifting insertions and deletions (indels) may also participate in adaptation through gene disruption, which could be reversed by secondary introduction of a frame-restoring indel. We present ScarTrek, a program that scans genomic data for indels, including those that together disrupt and restore a gene’s reading frame, producing “frame-shift scars” suggestive of reversible gene inactivation. We use ScarTrek to analyze 5977 clinical M. tuberculosis isolates. We show that indel frequency inversely correlates with genomic linguistic complexity and varies with gene-position and gene-essentiality. Using ScarTrek, we detect 74 unique frame-shift scars in 48 genes, with a 3.74% population-level incidence of unique scar events. We find multiple scars in the ESX-1 gene cluster. Six scars show evidence of convergent evolution while the rest shared a common ancestor. Our results suggest that sequential indels are a mechanism for reversible gene silencing and adaptation in M. tuberculosis.

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