A Symmetric Length-Aware Enrichment Test

2016 ◽  
Vol 23 (6) ◽  
pp. 508-525 ◽  
Author(s):  
David Manescu ◽  
Uri Keich
Keyword(s):  
Enrichment Test

scholarly journals BgeeDB, an R package for retrieval of curated expression datasets and for gene list expression localization enrichment tests

F1000Research ◽  
2016 ◽  
Vol 5 ◽  
pp. 2748 ◽  
Author(s):  
Andrea Komljenovic ◽  
Julien Roux ◽  
Marc Robinson-Rechavi ◽  
Frederic B. Bastian
Keyword(s):  
Gene Expression ◽  
Gene List ◽  
R Package ◽  
Rna Seq ◽  
Anatomical Structures ◽  
Enrichment Test ◽  
Expression Of Genes ◽  
Affymetrix Microarrays ◽  
New Gene

BgeeDB is a collection of functions to import into R re-annotated, quality-controlled and reprocessed expression data available in the Bgee database. This includes data from thousands of wild-type healthy samples of multiple animal species, generated with different gene expression technologies (RNA-seq, Affymetrix microarrays, expressed sequence tags, and in situ hybridizations). BgeeDB facilitates downstream analyses, such as gene expression analyses with other Bioconductor packages. Moreover, BgeeDB includes a new gene set enrichment test for preferred localization of expression of genes in anatomical structures (“TopAnat”). Along with the classical Gene Ontology enrichment test, this test provides a complementary way to interpret gene lists. Availability: http://www.bioconductor.org/packages/BgeeDB/

scholarly journals BiFET: sequencing Bias-free transcription factor Footprint Enrichment Test

2018 ◽  
Vol 47 (2) ◽  
pp. e11-e11 ◽  
Author(s):  
Ahrim Youn ◽  
Eladio J Marquez ◽  
Nathan Lawlor ◽  
Michael L Stitzel ◽  
Duygu Ucar
Keyword(s):  
Transcription Factor ◽  
Enrichment Test ◽  
Sequencing Bias

scholarly journals Refining Bulk Segregant Analyses: Ontology-Mediated Discovery of Flowering Time Genes in Brassica oleracea

2021 ◽  
Author(s):  
Rutger Aldo Vos ◽  
Karin van Veen ◽  
Eric Schranz ◽  
Klaas Vrieling ◽  
Peter G. L. Klinkhamer ◽  
...  
Keyword(s):  
Gene Ontology ◽  
Circadian Rhythm ◽  
Flowering Time ◽  
Quantitative Trait ◽  
Complex Traits ◽  
Significant Variation ◽  
Bulk Segregant Analysis ◽  
Source Code ◽  
Enrichment Test ◽  
Flowering Onset

Bulk segregant analysis (BSA) can help identify quantitative trait loci (QTLs), but this may result in substantial bycatch of functionally irrelevant genes. Here we develop a Gene Ontology-mediated approach to zoom in on specific markers implicated in flowering time from among QTLs identified by BSA of the giant woody Jersey kale phenotyped in four bulks of flowering onset. Our BSA yielded tens of thousands of candidate genes. We reduced this by two orders of magnitude by focusing on genes annotated with terms contained within relevant subgraphs of the Gene Ontology. A further enrichment test led to the pathway for circadian rhythm in plants. The genes that enriched this pathway are attested from previous research as regulating flowering time. Some of these genes were also identified as having functionally significant variation compared to Arabidopsis. We validated and confirmed our ontology-mediated results through a more targeted, homology-based approach. However, our ontology-mediated approach produced additional genes of putative importance, showing that the approach aids in exploration and discovery. We view our method as potentially applicable to the study of other complex traits and therefore make our workflows available as open-source code and a reusable Docker container.

scholarly journals Adaptation to Extreme Environments in an Admixed Human Population from the Atacama Desert

2019 ◽  
Vol 11 (9) ◽  
pp. 2468-2479 ◽  
Author(s):  
Lucas Vicuña ◽  
Mario I Fernandez ◽  
Cecilia Vial ◽  
Patricio Valdebenito ◽  
Eduardo Chaparro ◽  
...  
Keyword(s):  
Native American ◽  
Ribosome Biogenesis ◽  
Coronary Thrombosis ◽  
Extreme Environments ◽  
Atacama Desert ◽  
Inorganic Arsenic ◽  
Urinary Bladder Cancer ◽  
Enrichment Test ◽  
Genome Wide ◽  
Before And After

Abstract Inorganic arsenic (As) is a toxic xenobiotic and carcinogen associated with severe health conditions. The urban population from the Atacama Desert in northern Chile was exposed to extremely high As levels (up to 600 µg/l) in drinking water between 1958 and 1971, leading to increased incidence of urinary bladder cancer (BC), skin cancer, kidney cancer, and coronary thrombosis decades later. Besides, the Andean Native-American ancestors of the Atacama population were previously exposed for millennia to elevated As levels in water (∼120 µg/l) for at least 5,000 years, suggesting adaptation to this selective pressure. Here, we performed two genome-wide selection tests—PBSn1 and an ancestry-enrichment test—in an admixed population from Atacama, to identify adaptation signatures to As exposure acquired before and after admixture with Europeans, respectively. The top second variant selected by PBSn1 was associated with LCE4A-C1orf68, a gene that may be involved in the immune barrier of the epithelium during BC. We performed association tests between the top PBSn1 hits and BC occurrence in our population. The strongest association (P = 0.012) was achieved by the LCE4A-C1orf68 variant. The ancestry-enrichment test detected highly significant signals (P = 1.3 × 10−9) mapping MAK16, a gene with important roles in ribosome biogenesis during the G1 phase of the cell cycle. Our results contribute to a better understanding of the genetic factors involved in adaptation to the pathophysiological consequences of As exposure.

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