In Vitro Induction of Human Embryonic Stem Cells into the Midbrain Dopaminergic Neurons and Transplantation in Cynomolgus Monkey

2019 ◽  
Vol 21 (6) ◽  
pp. 285-295
Author(s):  
Xiaoyan Qiu ◽  
Yingquan Liu ◽  
Xiong Xiao ◽  
Jingjing He ◽  
Huiyun Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Cynomolgus Monkey ◽  
Dopaminergic Neurons ◽  
Embryonic Stem ◽  
Midbrain Dopaminergic Neurons ◽  
In Vitro Induction

In vitro induction of human embryonic stem cells into hepatocyte-like cells

2007 ◽  
Vol 52 (23) ◽  
pp. 3221-3226
Author(s):  
HaiYun Pei ◽  
YunFang Wang ◽  
YinXiang Yang ◽  
HongMei Peng ◽  
JiaFei Xi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
In Vitro Induction

Generation of dopaminergic neurons in vitro from human embryonic stem cells treated with neurotrophic factors

2004 ◽  
Vol 359 (1-2) ◽  
pp. 99-103 ◽  
Author(s):  
Sepill Park ◽  
Keum Sil Lee ◽  
Young Jae Lee ◽  
Hyun Ah Shin ◽  
Hwang Yoon Cho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Neurotrophic Factors ◽  
Dopaminergic Neurons ◽  
Embryonic Stem

scholarly journals Human embryonic stem cells-derived dopaminergic neurons transplanted in parkinsonian monkeys recover dopamine levels and motor behavior

2020 ◽  
Author(s):  
Adolfo López-Ornelas ◽  
Itzel Escobedo-Avila ◽  
Gabriel Ramírez-García ◽  
Rolando Lara-Rodarte ◽  
César Meléndez-Ramírez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Dopaminergic Neurons ◽  
Motor Behavior ◽  
Imaging Techniques ◽  
Embryonic Stem ◽  
Mean Diffusivity ◽  
Positron Emission

Human embryonic stem cells (hESCs) differentiate into specialized cells, including midbrain dopaminergic neurons (DAN). Non-human primates (NHPs) injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine develop some alterations observed in Parkinson’s disease (PD) patients. We obtained DAN from hESCs and confirmed that they express dopaminergic markers, generate action potentials, and release dopamine (DA) in vitro. DAN were transplanted bilaterally in the putamen of parkinsonian NHPs. After grafting, animals improved motor behavior, evaluated by the HALLWAY task, and in agreement with this recovery, DA release was detected by microdialysis. Imaging techniques revealed changes in fractional anisotropy and mean diffusivity in magnetic resonance imaging and higher 11C-DTBZ binding in positron-emission tomography scans, associated with grafts. Postmortem analysis showed that transplanted DAN survived over ten months in the putamen, without developing tumors, in the immunosuppressed NHPs. These results indicate that cell replacement therapy with hESCs-derived DAN causes long-term biochemical, anatomical, and physiological improvements in this model of PD.

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