Human Menstrual Blood Stem Cell-Derived Granulosa Cells Participate in Ovarian Follicle Formation in a Rat Model of Premature Ovarian Failure In Vivo

2019 ◽  
Vol 21 (5) ◽  
pp. 249-259 ◽  
Author(s):  
Parastoo Noory ◽  
Shadan Navid ◽  
Bagher Minaee Zanganeh ◽  
Ali Talebi ◽  
Maryam Borhani-Haghighi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Granulosa Cells ◽  
Rat Model ◽  
Premature Ovarian Failure ◽  
Ovarian Follicle ◽  
Ovarian Failure ◽  
Blood Stem Cell ◽  
Menstrual Blood

Menstrual blood CD146 + mesenchymal stem cells reduced fibrosis rate in the rat model of premature ovarian failure

2021 ◽  
Author(s):  
Nahideh Nazdikbin Yamchi ◽  
Reza Rahbarghazi ◽  
Alberto Miranda Bedate ◽  
Mahdi Mahdipour ◽  
Mohammad Nouri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Rat Model ◽  
Premature Ovarian Failure ◽  
Ovarian Failure ◽  
Menstrual Blood

scholarly journals Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Siwen Zhang ◽  
Boxian Huang ◽  
Peng Su ◽  
Qiyuan Chang ◽  
Pingping Li ◽  
...  
Keyword(s):  
Granulosa Cells ◽  
Rat Model ◽  
Stromal Cells ◽  
Premature Ovarian Insufficiency ◽  
Menstrual Blood ◽  
Follicle Development ◽  
Ovarian Insufficiency ◽  
Estrous Cyclicity

Abstract Background Premature ovarian insufficiency (POI) is one of the major causes of infertility. We previously demonstrated that transplantation of menstrual blood-derived stromal cells (MenSCs) effectively improved ovarian function in a murine model of POI. Recent studies indicated that mesenchymal stem cell-derived exosomes were important components in tissue repair. In this study, we investigated the therapeutic effects of MenSCs-derived exosomes (MenSCs-Exos) in a rat model of POI and its mechanism in restoring ovulation. Methods Ovaries of 4.5-day-old Sprague Dawley rats (SD rats) were cultured in vitro to evaluate the effects of MenSCs-Exos exposure on early follicle development. Furthermore, POI in rats was induced by intraperitoneal administration of 4-vinylcyclohexene diepoxide (VCD). Forty-eight POI rats were randomly assigned to four groups, each receiving a different treatment: PBS, MenSCs, MenSCs-Exos, and Exo-free culture supernatant of MenSCs. Estrous cyclicity, ovarian morphology, follicle dynamics, serum hormones, pregnancy outcomes, and molecular changes were investigated. Results Exposure to MenSCs-Exos promoted the proliferation of granulosa cells in primordial and primary follicles in vitro and increased the expression of early follicle markers Deleted In Azoospermia Like (DAZL) and Forkhead Box L2 (FOXL2) while inhibiting follicle apoptosis. In vivo, MenSCs-Exos transplantation effectively promoted follicle development in the rat model of POI and restored the estrous cyclicity and serum sex hormone levels, followed by improving the live birth outcome. In addition, transplantation of MenSCs-Exos regulated the composition of the ovarian extracellular matrix and accelerated the recruitment of dormant follicles in the ovarian cortex and increased proliferation of granulosa cells in these follicles. Conclusion MenSCs-Exos markedly promoted follicle development in vitro and in vivo and restored fertility in POI rats, suggesting a restorative effect on ovarian functions. The therapeutic effect of MenSCs-Exos transplantation was sustainable, consistent with that of MenSCs transplantation. Our results suggested that MenSCs-Exos transplantation may be a promising cell-free bioresource in the treatment of POI.

scholarly journals Embryonic Stem Cell–Derived Granulosa Cells Participate in Ovarian Follicle Formation In Vitro and In Vivo

2013 ◽  
Vol 20 (5) ◽  
pp. 524-535 ◽  
Author(s):  
Dori C. Woods ◽  
Yvonne A. R. White ◽  
Yuichi Niikura ◽  
Sorapop Kiatpongsan ◽  
Ho-Joon Lee ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Granulosa Cells ◽  
Ovarian Follicle ◽  
Embryonic Stem

scholarly journals p27kip1 (Cyclin-Dependent Kinase Inhibitor 1B) Controls Ovarian Development by Suppressing Follicle Endowment and Activation and Promoting Follicle Atresia in Mice

2007 ◽  
Vol 21 (9) ◽  
pp. 2189-2202 ◽  
Author(s):  
Singareddy Rajareddy ◽  
Pradeep Reddy ◽  
Chun Du ◽  
Lian Liu ◽  
Krishna Jagarlamudi ◽  
...  
Keyword(s):  
Premature Ovarian Failure ◽  
Molecular Mechanisms ◽  
Ovarian Development ◽  
Ovarian Follicle ◽  
Cyclin A ◽  
Ovarian Failure ◽  
Cyclin Dependent Kinase ◽  
Female Reproduction ◽  
Oocyte Growth ◽  
Follicle Atresia

Abstract In humans, the molecular mechanisms underlying ovarian follicle endowment and activation, which are closely related to the control of female reproduction, occurrence of menopause, and related diseases such as premature ovarian failure, are poorly understood. In the current study, we provide several lines of genetic evidence that the cyclin-dependent kinase (Cdk) inhibitor 1B (commonly known as p27kip1 or p27) controls ovarian development in mice by suppressing follicle endowment and activation, and by promoting follicle death. In p27-deficient (p27−/−) mice, postnatal follicle assembly was accelerated, and the number of endowed follicles was doubled as compared with p27+/+ mice. Moreover, in p27−/− ovaries the primordial follicle pool was prematurely activated once it was endowed, and at the same time the massive follicular death that occurs before sexual maturity was rescued by loss of p27. In early adulthood, however, the overactivated follicular pool in p27−/− ovaries was largely depleted, causing premature ovarian failure. Furthermore, we have extensively studied the molecular mechanisms underlying the above-mentioned phenotypes seen in p27−/− ovaries and have found that p27 controls follicular development by several distinct mechanisms at different stages of development of the ovary. For example, p27 controls oocyte growth by suppressing the functions of Cdk2/Cdc2-cyclin A/E1 in oocytes that are arrested at the diplotene stage of meiosis I. This function of p27 is distinct from its well-known role as a suppressor of cell cycle progression. In addition, we have found that p27 activates the caspase-9-caspase-3-caspase-7-poly (ADP-ribose) polymeraseapoptotic cascade by inhibiting Cdk2/Cdc2-cyclin A/B1 kinase activities in follicles, thereby inducing follicle atresia. Our results suggest that the p27 gene is important in determining mammalian ovarian development. This study therefore provides insight into ovary-borne genetic aberrations that cause defects in folliculogenesis and infertility in humans.

scholarly journals Investigation of the Relation between Follicular Atresia and Granulosa Cells in Terms of Cell Death Mechanisms in Premature Ovarian Failure Model

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1529 ◽  
Author(s):  
Damla Akogullari ◽  
Elgin Turkoz Uluer ◽  
H. Seda Vatansever
Keyword(s):  
Cell Death ◽  
Granulosa Cells ◽  
Premature Ovarian Failure ◽  
Ovarian Failure ◽  
Blood Levels ◽  
Failure Model ◽  
Follicular Atresia ◽  
Treatment Protocols ◽  
New Treatment ◽  
Cell Death Mechanisms

Premature Ovarian Failure; is characterized by the dysfunction or early depletion of ovarian reserves due to follicular loss in the ovary in women under age of 40. POF is the important cause of infertility and its etiology is still not clearly understood. Investigation of cell death mechanisms (CDM) that play a role in the follicular atresia (FA) triggered by excessive loss of granulosa cells (GCs) that provide metabolic support for oocyte and follicle development in the ovary will help to understand POF etiology. It was known that apoptosis and autophagy play a role in FA. Recent studies have shown that paraptosis, associated with endoplasmic reticulum stress (ERS), also exist in FA. POF model was established in C57BL/6 female mice by CTX and it was confirmed by increased follicle stimulating hormone (FSH), luteal hormone (LH) and decreased estradiol (E2) blood levels and follicle count. According to the results of the immunohistochemistry (IHC) cell death markers were significantly more expressed than control (C) and sham (S) groups in the POF model. In addition, more apoptotic cells were observed in the POF group compared to C and S in the TUNEL analysis. In consequence of this study apoptosis and autophagy as well as paraptosis play a role in the FA leading to POF, will help to develop new treatment protocols.

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