MC1568 Enhances Histone Acetylation During Oocyte Meiosis and Improves Development of Somatic Cell Nuclear Transfer Embryos in Pig

2018 ◽  
Vol 20 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Huili Wang ◽  
Wei Cui ◽  
Chunhua Meng ◽  
Jun Zhang ◽  
Yinxia Li ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Histone Acetylation ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Oocyte Meiosis

Effects of Scriptaid on the Histone Acetylation, DNA Methylation and Development of Buffalo Somatic Cell Nuclear Transfer Embryos

2015 ◽  
Vol 17 (5) ◽  
pp. 404-414 ◽  
Author(s):  
Hongliang Sun ◽  
Fenghua Lu ◽  
Peng Zhu ◽  
Xiaohua Liu ◽  
Mingming Tian ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Somatic Cell ◽  
Histone Acetylation ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer

50 EFFECTS OF REPROGRAMMING TRANSCRIPTION FACTORS AND CHEMICAL INHIBITORS ON THE DEVELOPMENT OF BOVINE SOMATIC CELL NUCLEAR TRANSFER EMBRYOS

2013 ◽  
Vol 25 (1) ◽  
pp. 172
Author(s):  
B.-C. Yang ◽  
J.-H. An ◽  
H. K. Chung ◽  
S.-J. Jo ◽  
H.-J. Chung ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Histone Acetylation ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Birth Rate ◽  
Development Rate ◽  
Control Group ◽  
Transfected Cells ◽  
Development Rates ◽  
Chemical Inhibitors

Currently, somatic cell nuclear transfer (SCNT) is regarded as the most efficient technique to produce transgenic cattle. However, compared with other techniques, such as IVF and in vivo embryo, the birth rate of SCNT embryos has been disappointingly low. Because incomplete epigenetic reprogramming in SCNT embryos has been suggested as a major reason for this low birth rate, we attempted to improve the success rate by using several lines of donor cells that were reprogrammed by gene transfection and an inhibitor treatment. First, the bovine ear fibroblast cells were transfected with vectors (pCX-OKS-2A and pCX-Myc) carrying 4 genes (Oct-4, Klf4, Sox2, and c-Myc; OKSM). Subsequently, transfected cells were incubated in the presence of one of the following chemical inhibitors: (1) trichostatin A (TSA, 20 nM), (2) 5-aza-20-deoxycytidine (5-aza-dC, 10 µM), or (3) GSK-3/MEK inhibitor (2i; GSK-3, 3 µM and MEK, 1 µM) for 7 days. Throughout the incubation period, cells in each treatment group were analyzed for their histone acetylation status. Subsequently, transfected cells were used as nucleus donors in SCNT and their development rates to the blastocyst stage were estimated. In the present study, it was found that the histone acetylation (H3K9/K14) levels were generally higher in treated groups compared with nontreated control groups. Regarding blastocyst development rate, the OKSM/5-aza-dC (10/49, 20.1 ± 5.9%) combination was better than the other groups: OKSM/TSA (8/77, 11.1 ± 6.9%), OKSM/2i (8/84, 9.5 ± 1.5%), OKSM (6/50, 12.6 ± 6.5%), and negative control group (9/94, 9.6 ± 3.8%; P < 0.05). Embryo development rates appeared to be higher in OKSM/inhibitor-treated groups, although the rate was significantly lower (P < 0.05) in OKSM/2i. In conclusion, our data suggest that transfection with 4 genes and incubating with 5-aza-dC can significantly improve the development rate of bovine SCNT embryos.

Effects of scriptaid on the histone acetylation of buffalo oocytes and their ability to support the development of somatic cell nuclear transfer embryos

2015 ◽  
Vol 83 (7) ◽  
pp. 1219-1225 ◽  
Author(s):  
Hongliang Sun ◽  
Fenghua Lu ◽  
Xiaohua Liu ◽  
Mingming Tian ◽  
Ziyun Ruan ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Histone Acetylation ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer

Trichostatin A Improves Histone Acetylation in Bovine Somatic Cell Nuclear Transfer Early Embryos

2008 ◽  
Vol 10 (3) ◽  
pp. 371-380 ◽  
Author(s):  
Amy E. Iager ◽  
Neli P. Ragina ◽  
Pablo J. Ross ◽  
Zeki Beyhan ◽  
Kerrianne Cunniff ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Histone Acetylation ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Trichostatin A ◽  
Early Embryos
Sign in / Sign up

Export Citation Format

Share Document