Improved Isolation, Proliferation, and Differentiation Capacity of Mouse Ovarian Putative Stem Cells

2017 ◽  
Vol 19 (2) ◽  
pp. 132-144 ◽  
Author(s):  
Hossein Yazdekhasti ◽  
Marzieh Agha Hosseini ◽  
Zahra Rajabi ◽  
Soraya Parvari ◽  
Mojdeh Salehnia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Differentiation Capacity ◽  
Proliferation And Differentiation

scholarly journals Mechanical Stress Stimulates the Osteo/Odontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 1/2 and JNK MAPK Pathways

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Chao Mu ◽  
Taohong Lv ◽  
Zilu Wang ◽  
Shu Ma ◽  
Jie Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mechanical Stress ◽  
Background Information ◽  
Ultrastructural Changes ◽  
Mechanical Stimuli ◽  
Human Stem Cells ◽  
Differentiation Capacity ◽  
Mapk Pathways ◽  
Proliferation And Differentiation ◽  
Apical Papilla

Background Information. Stem cells from apical papilla (SCAPs) are a potent candidate for the apexogenesis/apexification due to their multiple differentiation capacity. During the orthodontic treatment of developing teeth, SCAPsin vivoare usually subjected to the cyclic stress induced by compression forces. However, it remains unclear whether mechanical stress can affect the proliferation and differentiation of human SCAPs.Results. Human SCAPs were isolated and stimulated by 200 g mechanical stimuli for 30 min and their proliferation and differentiation capacity were evaluatedin vitroat different time points. MTT and FCM results demonstrated that cell proliferation was enhanced, while TEM findings showed the morphological and ultrastructural changes in stress-treated SCAPs. ALP activity and mineralization capacity of stress-treated SCAPs were upregulated . In the meantime, higher odontogenic and osteogenic differentiation were found in stress-treated SCAPs by real-time RT-PCR and Western blot, as indicated by the expression of related markers at both mRNA and protein levels. Moreover, the protein expressions of pJNK and pERK MAPK pathways were upregulated.Conclusion. Together, these findings suggest that mechanical stress is an important factor affecting the proliferation and differentiation of SCAPs via the activation of ERK and JNK signaling pathway.

195: Aging Effects on the Proliferation and Differentiation Capacity of Adipose Stem Cells

2007 ◽  
Vol 177 (4S) ◽  
pp. 65-66
Author(s):  
Valerie A. Arboleda ◽  
Suny Kun ◽  
Vanda Lopez ◽  
Rang Zhang ◽  
Larissa V. Rodriguez
Keyword(s):  
Stem Cells ◽  
Adipose Stem Cells ◽  
Aging Effects ◽  
Differentiation Capacity ◽  
Proliferation And Differentiation

Effects of different media on proliferation and differentiation capacity of canine, equine and porcine adipose derived stem cells

2012 ◽  
Vol 93 (1) ◽  
pp. 457-462 ◽  
Author(s):  
Christina Schwarz ◽  
Uta Leicht ◽  
Christine Rothe ◽  
Inga Drosse ◽  
Volker Luibl ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Derived Stem Cells ◽  
Differentiation Capacity ◽  
Proliferation And Differentiation
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