Effect of Long-Term Exposure of Donor Nuclei to the Oocyte Cytoplasm on Production of Cloned Mice Using Serial Nuclear Transfer

2016 ◽  
Vol 18 (6) ◽  
pp. 382-389 ◽  
Author(s):  
Sayaka Wakayama ◽  
Yoshiaki Tanabe ◽  
Hiroaki Nagatomo ◽  
Eiji Mizutani ◽  
Satoshi Kishigami ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
Oocyte Cytoplasm ◽  
Serial Nuclear Transfer

Secretion of interferon-tau by bovine embryos in long-term culture: comparison of in vivo derived, in vitro produced, nuclear transfer and demi-embryos

1999 ◽  
Vol 55 (3-4) ◽  
pp. 151-162 ◽  
Author(s):  
M Stojkovic ◽  
M Büttner ◽  
V Zakhartchenko ◽  
J Riedl ◽  
H.-D Reichenbach ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
Bovine Embryos ◽  
Interferon Tau ◽  
Long Term Culture

scholarly journals Bovine Oocyte Cytoplasm Supports Development of Embryos Produced by Nuclear Transfer of Somatic Cell Nuclei from Various Mammalian Species1

1999 ◽  
Vol 60 (6) ◽  
pp. 1496-1502 ◽  
Author(s):  
Tanja Dominko ◽  
Maissam Mitalipova ◽  
Brad Haley ◽  
Zeki Beyhan ◽  
Erdogan Memili ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Nuclear Transfer ◽  
Bovine Oocyte ◽  
Cell Nuclei ◽  
Oocyte Cytoplasm

Production of cloned goats by nuclear transfer of cumulus cells and long-term cultured fetal fibroblast cells into abattoir-derived oocytes

2006 ◽  
Vol 73 (7) ◽  
pp. 834-840 ◽  
Author(s):  
Guo-Cheng Lan ◽  
Zhong-Le Chang ◽  
Ming-Jiu Luo ◽  
Yun-Liang Jiang ◽  
Dong Han ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
Cumulus Cells ◽  
Fibroblast Cells ◽  
Fetal Fibroblast

From the germinal cells to the newborn animal: The transmission of genes and life through the generations

2003 ◽  
Vol 51 (3) ◽  
pp. 371-384
Author(s):  
P. V. Drion ◽  
O. Szenci ◽  
F. Ectors ◽  
D. Wirth ◽  
Zs. Perényi ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
Fetal Mortality ◽  
Newborn Animal ◽  
Multiple Ovulation ◽  
Early Appearance ◽  
Early Embryos ◽  
Germinal Cells

The technology of reproduction progressed considerably during the last decade, leading to a certain availability of in vitro methods for fertilisation, oocyte maturation and embryo culture. The most spectacular manipulations are cloning and transgenesis. This review focuses on the early appearance of germinal cell precursors and the long-standing fate of gametes in mammals. The evident complexity and long-term programming of events in gametes and early embryos explain part of the difficulties encountered during the development of in vitro and in vivo methods such as multiple ovulation and embryo transfer (MOET), oestrus synchronisation, ovulation induction, superovulation, in vitro maturation and fertilisation, cryopreservation, transgenesis, nuclear transfer and cloning) and the occurrence of unexpected alterations of development, e.g. embryonic or fetal mortality, large-weight newborn syndrome and other dysregulations in imprinting or DNA transmission.

47 BLASTOCYST DEVELOPMENT RATE OF CLONED BOVINE EMBRYOS USING SERIAL NUCLEAR TRANSFER OF CELLS CONTAINING AN X-AUTOSOME-TRANSLOCATED CHROMOSOME t(Xp+;23q-)

2006 ◽  
Vol 18 (2) ◽  
pp. 132
Author(s):  
W. A. King ◽  
B.-G. Jeon ◽  
D. H. Betts
Keyword(s):  
Nuclear Transfer ◽  
Primary Cultures ◽  
Calf Serum ◽  
Development Rate ◽  
Blastocyst Development ◽  
Cell Numbers ◽  
2Nd Generation ◽  
Autosome Translocation ◽  
Chi Square Analysis ◽  
Serial Nuclear Transfer

Somatic cell nuclear transfer (SCNT) has been utilized to study various genetic and epigenetic contributions of specific biomedical diseases and developmental events by using various donor cell types such as mature lymphocytes, brain tumor cells, and other unique genotypes. Previously, we produced cloned fetuses and offspring derived from SCNT of adult ear skin fibroblasts obtained from a sub-fertile cow harboring an X-autosome translocation as a model to study X-inactivation and chromosome dynamics during female meiosis. The aim of this study was to assess the cloning efficiency of the fibroblasts derived from a cloned calf with the X-autosome translocation t(Xp+;23q-) compared to the original adult fibroblast donor containing the same chromosome translocation. Primary cultures of cells were established in DMEM +15% fetal calf serum (FCS). To serve as nuclear donors, cells at 5-7 passages were cultured for 5 days until confluent. Oocytes matured for 18 h in TCM-199 with hormones were removed of their chromatin, and reconstructed by transfer of donor cells and fusion with two DC pulses (1.2 kV/cm, 15 �s), delivered by a BTX 2000 Electro Cell Minupulator (BTX, Inc., San Diego, CA, USA), in 0.28 M mannitol containing 0.01 mM MgCl2. After 1 h of fusion, the eggs were activated with 5.5 �M ionomycin for 5 min, followed by 11 �g/mL cyclohexamide for 5 h. The eggs were cultured for 9 days in L-SOF at 39�C in a humidified atmosphere of 5% CO2, 5% O2, 90% N2. Chi-square analysis revealed no significant (P > 0.05) differences in the rates of cleavage, blastocyst frequencies, and cell numbers between the 1st and 2nd generation cloned embryos. Cleavage rates were 87.4% and 85.4% for 1st and 2nd generation cloned embryos, respectively. The frequencies of blastocyst development and hatched blastocyst formation on Day 9 were 41.4% (91/220) and 38.7% (92/238), and 26.4% (58/220) and 22.7% (54/238) for the 1st and 2nd generation cloned embryos, respectively. The numbers of total cells and inner cell mass (ICM) cells of Day 9 blastocysts were 183 and 52, respectively, in the 1st generation embryos and 167 and 51 cells in the 2nd-generation cloned embryos. In summary, 2nd generation cloned embryos derived from fibroblasts of a cloned calf with an X-autosome translocated chromosome showed embryo development and cell numbers similar to those of the 1st generation clones. These results demonstrate that serial nuclear transfer does not improve the blastocyst development rate of cloned embryos containing the X-autosome translocation t(Xp+;23q-). This work was funded by OCAG, OMAF, and CRC.

Cryopreservation of manipulated embryos: tackling the double jeopardy

2009 ◽  
Vol 21 (1) ◽  
pp. 45 ◽  
Author(s):  
A. Dinnyes ◽  
T. L. Nedambale
Keyword(s):  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryo Cryopreservation ◽  
Double Jeopardy ◽  
Breeding Technology ◽  
Mammalian Embryos ◽  
Long Term Consequences ◽  
Transgenic Embryos

The aim of the present review is to provide information to researchers and practitioners concerning the reasons for the altered viability and the medium- and long-term consequences of cryopreservation of manipulated mammalian embryos. Embryo manipulation is defined herein as the act or process of manipulating mammalian embryos, including superovulation, AI, IVM, IVF, in vitro culture, intracytoplasmic sperm injection, embryo biopsy or splitting, somatic cell nuclear transfer cloning, the production of sexed embryos (by sperm sexing), embryo cryopreservation, embryo transfer or the creation of genetically modified (transgenic) embryos. With advances in manipulation technologies, the application of embryo manipulation will become more frequent; the proper prevention and management of the resulting alterations will be crucial in establishing an economically viable animal breeding technology.

Production of a cloned calf using zona-free serial nuclear transfer

2006 ◽  
Vol 65 (2) ◽  
pp. 424-440 ◽  
Author(s):  
Vanessa J. Hall ◽  
Nancy T. Ruddock ◽  
Melissa A. Cooney ◽  
Natasha A. Korfiatis ◽  
R. Tayfur Tecirlioglu ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
Serial Nuclear Transfer

Embryonic and somatic cell cloning

1998 ◽  
Vol 10 (8) ◽  
pp. 639 ◽  
Author(s):  
Ian Wilmut ◽  
Lorraine Young ◽  
Keith H.S. Campbell
Keyword(s):  
Gene Expression ◽  
Nuclear Transfer ◽  
Genome Mapping ◽  
Specific Cell ◽  
Regulate Gene Expression ◽  
Donor Cells ◽  
Patient Will ◽  
Oocyte Cytoplasm ◽  
Regulate Gene

Revolutionary opportunities in biology, medicine and agriculture arise from the observation that offspring are obtained after nuclear transfer if somatic donor cells are induced to become quiescent. Exploitation of many of these opportunities will depend upon optimizing procedures for nuclear transfer. This may come about through an understanding of the means by which factors in the oocyte cytoplasm act upon the DNA of the transferred nucleus to regulate gene expression. Similarly, research will extend the procedure to other species. This technology may be used for embryo production, the introduction of genetic change and the derivation of cells needed to treat human diseases. Groups of genetically identical animals will be used in research to control genetic variation and to allow transfer of cells between individuals. In agriculture, production of a small number of clones will separate genetic and environmental effects, whereas production of larger numbers of offspring will disseminate genetic improvement from nucleus herds. Precise genetic modification will be achieved by site specific recombination in the donor cells before nuclear transfer. In all mammals it will become possible to define the role of any gene product and to analyse the mechanisms that regulate gene expression. Medical uses of these techniques will include the production of proteins needed to treat disease and the supply of organs such as hearts, livers and kidneys from pigs. As genome mapping projects identify loci associated with traits of commercial importance in agriculture then gene targeting will be used to study this effect. Finally, cells capable of differentiation into any of the tissues of a patient will provide treatment for diseases reflecting damage to a specific cell population that neither repairs nor replaces itself.

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