scholarly journals Impaired Neural Differentiation Potency by Retinoic Acid Receptor-α Pathway Defect in Induced Pluripotent Stem Cells

2014 ◽  
Vol 16 (6) ◽  
pp. 467-476 ◽  
Author(s):  
Pei-Shan Hou ◽  
Wen-Chin Huang ◽  
Wei Chiang ◽  
Wei-Che Lin ◽  
Chung-Liang Chien
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Neural Differentiation ◽  
Retinoic Acid Receptor ◽  
Acid Receptor ◽  
Induced Pluripotent ◽  
Retinoic Acid Receptor Α

scholarly journals Retinoic acid receptor γ activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium

2020 ◽  
Vol 55 (8) ◽  
pp. 763-774
Author(s):  
Yasufumi Koterazawa ◽  
Michiyo Koyanagi-Aoi ◽  
Keiichiro Uehara ◽  
Yoshihiro Kakeji ◽  
Takashi Aoi
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Retinoic Acid Receptor ◽  
Marker Genes ◽  
Acid Receptor ◽  
All Trans Retinoic Acid ◽  
Human Esophagus ◽  
Induced Pluripotent

Abstract Background The esophagus is known to be derived from the foregut. However, the mechanisms regulating this process remain unclear. In particular, the details of the human esophagus itself have been poorly researched. In this decade, studies using human induced pluripotent stem cells (hiPSCs) have proven powerful tools for clarifying the developmental biology of various human organs. Several studies using hiPSCs have demonstrated that retinoic acid (RA) signaling promotes the differentiation of foregut into tissues such as lung and pancreas. However, the effect of RA signaling on the differentiation of foregut into esophagus remains unclear. Methods We established a novel stepwise protocol with transwell culture and an air–liquid interface system for esophageal epithelial cell (EEC) differentiation from hiPSCs. We then evaluated the effect of all-trans retinoic acid (ATRA), which is a retinoic acid receptor (RAR)α, RARβ and RARγ agonist, on the differentiation from the hiPSC-derived foregut. Finally, to identify which RAR subtype was involved in the differentiation, we used synthetic agonists and antagonists of RARα and RARγ, which are known to be expressed in esophagus. Results We successfully generated stratified layers of cells expressing EEC marker genes that were positive for lugol staining. The enhancing effect of ATRA on EEC differentiation was clearly demonstrated with quantitative reverse transcription polymerase chain reaction, immunohistology, lugol-staining and RNA sequencing analyses. RARγ agonist and antagonist enhanced and suppressed EEC differentiation, respectively. RARα agonist had no effect on the differentiation. Conclusion We revealed that RARγ activation promotes the differentiation of hiPSCs-derived foregut into EECs.

scholarly journals Early Inhibition of Retinoic Acid Signaling Rapidly Generates Cardiomyocytes Expressing Ventricular Markers from Human Induced Pluripotent Stem Cells

2019 ◽  
Author(s):  
Pranav Machiraju ◽  
Joshua Huang ◽  
Fatima Iqbal ◽  
Yiping Liu ◽  
Xuemei Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Troponin T ◽  
Muscle Protein ◽  
Retinoic Acid Receptor ◽  
Human Ipscs ◽  
Induced Pluripotent

SUMMARYCurrent protocols for the differentiation of cardiomyocytes from human induced pluripotent stem cells (iPSCs) generally require prolonged time in culture and result in heterogeneous cellular populations. We present a method for the generation of beating cardiomyocytes expressing specific ventricular markers after just 14 days. Addition of the pan-retinoic acid receptor inverse agonist BMS 493 to human iPSCs for the first 8 days of differentiation resulted in increased protein expression of the ventricular isoform of myosin regulatory light chain (MLC2V) from 18.7% ± 1.72% to 55.8% ± 11.4% (p <0.0001) in cells co-expressing the cardiac muscle protein troponin T (TNNT2). Increased MLC2V expression was also accompanied by a slower beating rate (49.4 ± 1.53 vs. 93.0 ± 2.81 beats per minute, p <0.0001) and increased contraction amplitude (201% ± 8.33% vs. 100% ± 10.85%, p <0.0001) compared to untreated cells. Improved directed differentiation will improve in vitro cardiac modeling.

scholarly journals Impaired Neural Differentiation of Induced Pluripotent Stem Cells Generated from a Mouse Model of Sandhoff Disease

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e55856 ◽  
Author(s):  
Yasuhiro Ogawa ◽  
Makoto Tanaka ◽  
Miho Tanabe ◽  
Toshihiro Suzuki ◽  
Tadayasu Togawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mouse Model ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Neural Differentiation ◽  
Sandhoff Disease ◽  
Induced Pluripotent
Sign in / Sign up

Export Citation Format

Share Document