Plasticity of Marrow Mesenchymal Stem Cells from Human First-Trimester Fetus: From Single-Cell Clone to Neuronal Differentiation

2011 ◽  
Vol 13 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Yihua Zhang ◽  
Wenzheng Shen ◽  
Bingjie Sun ◽  
Changrong Lv ◽  
Zhongying Dou
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Single Cell ◽  
Neuronal Differentiation ◽  
Cell Clone ◽  
First Trimester ◽  
Single Cell Clone ◽  
Marrow Mesenchymal Stem Cells

Safrole oxide induced neuronal differentiation of rat bone-marrow mesenchymal stem cells by elevating Hsp70

Gene ◽  
2012 ◽  
Vol 509 (1) ◽  
pp. 85-92 ◽  
Author(s):  
YanChun Zhao ◽  
Jie Xin ◽  
ChunHui Sun ◽  
BaoXiang Zhao ◽  
Jing Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Neuronal Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Rat Bone

scholarly journals Dissection of cellular communication between human primary osteoblasts and bone marrow mesenchymal stem cells in vivo at single-cell resolution

2022 ◽  
Author(s):  
Ying Liu ◽  
Yan Chen ◽  
Xiao-Hua Li ◽  
Tian-Peng Li ◽  
Chong Cao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Single Cell ◽  
Interaction Analysis ◽  
Receptor Interaction ◽  
Negative Feedback Regulation ◽  
Marrow Mesenchymal Stem Cells

Abstract BackgroundOsteoblasts are derived from bone marrow mesenchymal stem cells (BMMSCs) and play important role in bone remodeling. While our previous studies have investigated the cell subtypes and heterogeneity in osteoblasts and BMMSCs separately, cell-to-cell communications between osteoblasts and BMMSCs in vivo in humans have not been characterized.ResultsIn this study, we performed a systematic integration analysis with our single-cell RNA sequencing (scRNA-seq) transcriptomes data from BMMSCs and osteoblasts. We successfully identified a novel preosteoblasts subtype which highly expressed ATF3, CCL2, CXCL2 and IRF1. Biological functional annotations of the transcriptomes suggested that the novel preosteoblasts subtype may inhibit osteoblasts differentiation, maintain cells to a less differentiated status and recruit osteoclasts. Ligand-receptor interaction analysis showed strong interaction between mature osteoblasts and BMMSCs. Meanwhile, we found FZD1 was highly expressed in BMMSCs of osteogenic differentiation direction. WIF1 and SFRP4, which were highly expressed in mature osteoblasts were reported to inhibit osteogenic differentiation. We speculated that WIF1 and sFRP4 expressed in mature osteoblasts inhibited the binding of FZD1 to Wnt ligand in BMMSCs, thereby further inhibiting osteogenic differentiation of BMMSCs.ConclusionsAt the single cell level, this study provided insights into the cell-to-cell communications between BMMSCs and osteoblasts and mature osteoblasts may mediate negative feedback regulation of osteogenesis process.

scholarly journals Evaluation of APP695 Transgenic Mice Bone Marrow Mesenchymal Stem Cells Neural Differentiation for Transplantation

2015 ◽  
Vol 2015 ◽  
pp. 1-7
Author(s):  
Qian Li ◽  
Yanjie Jia ◽  
John Zhang ◽  
Jun Yang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Transgenic Mice ◽  
Western Blot ◽  
Neuronal Differentiation ◽  
Cell Transplantation ◽  
Neuronal Cell ◽  
Marrow Mesenchymal Stem Cells ◽  
Differentiation Efficiency

Objective. Even though there is a therapeutic potential to treat Alzheimer’s disease (AD) with neural cell replenishment and replacement, immunological rejections of stem cell transplantation remain a challenging risk. Autologous stem cells from AD patients however may prove to be a promising candidate. Therefore, we studied the neuronal differentiation efficiency of bone marrow mesenchymal stem cells (MSCs) from APP695 transgenic mice, which share features of human AD.Method. Cultured MSCs from APP695 transgenic mice are used; neuronal differentiation was assessed by immunocytochemistry and Western blot. Correlation with Notch signaling was examined. Autophage flux was assessed by western blot analysis.Results. MSCs from APP695 mice have higher neuronal differentiation efficiency than MSCs from wild type mice (WT MSCs). The expression of Notch-1 signaling decreased during the differentiation process. However, autophagy flux, which is essential for neuronal cell survival and neuronal function, was impaired in the neuronally differentiated counterparts of APP695 MSCs (APP695 MSCs–n).Conclusion. These results suggested autologous MSCs of APP690 mice may not be a good candidate for cell transplantation.

Cholinergic neuronal differentiation of bone marrow mesenchymal stem cells in rhesus monkeys

2010 ◽  
Vol 53 (5) ◽  
pp. 573-580 ◽  
Author(s):  
Ying Qi ◽  
FengYan Zhang ◽  
Ge Song ◽  
XueRong Sun ◽  
RuZhang Jiang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Neuronal Differentiation ◽  
Rhesus Monkeys ◽  
Marrow Mesenchymal Stem Cells
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