Knockdown of REG Iα Enhances the Sensitivity to 5-Fluorouracil of Colorectal Cancer Cells via Cyclin D1/CDK4 Pathway and BAX/BCL-2 Pathways

2019 ◽  
Vol 34 (6) ◽  
pp. 362-370 ◽  
Author(s):  
Yuwei Wang ◽  
Xiao Liu ◽  
Jingshu Liu ◽  
Tao Zhang
Keyword(s):  
Colorectal Cancer ◽  
Cyclin D1 ◽  
Cancer Cells ◽  
Colorectal Cancer Cells ◽  
Reg Iα

W1927 Reg IV Induces GSK-3β-β-Catenin-Tcf-4 Signaling and Increases Cyclin D1 Expression in Human Colorectal Cancer Cells

2009 ◽  
Vol 136 (5) ◽  
pp. A-755
Author(s):  
Kumar S. Bishnupuri ◽  
Qizhi Luo ◽  
Satheesh K. Sainathan ◽  
Jennifer Gross ◽  
Brian K. Dieckgraefe
Keyword(s):  
Colorectal Cancer ◽  
Cyclin D1 ◽  
Cancer Cells ◽  
Human Colorectal Cancer ◽  
Colorectal Cancer Cells ◽  
Gsk 3Β ◽  
Human Colorectal Cancer Cells

scholarly journals Anti-proliferative activity of A. Oxyphylla and its bioactive constituent nootkatone in colorectal cancer cells

BMC Cancer ◽  
2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Eunsu Yoo ◽  
Jaehak Lee ◽  
Pattawika Lertpatipanpong ◽  
Junsun Ryu ◽  
Chong-Tai Kim ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cyclin D1 ◽  
Cancer Cells ◽  
Bioactive Compound ◽  
Binding Activity ◽  
In Vitro Assays ◽  
Transcriptional Level ◽  
Wide Range ◽  
Colorectal Cancer Cells

Abstract Background A. oxyphylla extract is known to possess a wide range of pharmacological activites. However, the molecular mechanism of A. oxyphylla and its bioactive compound nootkatone in colorectal cancer is unknown. Methods Our study aims to examine the role of A. oxyphylla and its bioactive compound nootkatone, in tumor suppression using several in vitro assays. Results Both A. oxyphylla extract and nootkatone exhibited antiproliferative activity in colorectal cancer cells. A. oxyphylla displayed antioxidant activity in colorectal cancer cells, likely mediated via induction of HO-1. Furthermore, expression of pro-apoptotic protein NAG-1 and cell proliferative protein cyclin D1 were increased and decreased respectively in the presence of A. oxyphylla. When examined for anticancer activity, nootkatone treatment resulted in the reduction of colony and spheroid formation. Correspondingly, nootkatone also led to increased NAG-1 expression and decreased cyclin D1 expression. The mechanism by which nootkatone suppresses cyclin D1 involves protein level regulation, whereas nootkatone increases NAG-1 expression at the transcriptional level. In addition to having PPARγ binding activity, nootkatone also increases EGR-1 expression which ultimately results in enhanced NAG-1 promoter activity. Conclusion In summary, our findings suggest that nootkatone is an anti-tumorigenic compound harboring antiproliferative and pro-apoptotic activity.

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