Knockdown of Secreted Protein Acidic and Rich in Cysteine (SPARC) Expression Diminishes Radiosensitivity of Glioma Cells

2011 ◽  
Vol 26 (6) ◽  
pp. 705-715 ◽  
Author(s):  
Haiyan Liu ◽  
Haowen Zhang ◽  
Xin Jiang ◽  
Yan Ma ◽  
Yuanyuan Xu ◽  
...  
Keyword(s):  
Glioma Cells ◽  
Secreted Protein ◽  
Sparc Expression

Multivariate assessment of SPARC expression in resected pancreatic ductal adenocarcinoma to identify subgroups that are sensitive to adjuvant gemcitabine.

2017 ◽  
Vol 35 (4_suppl) ◽  
pp. 297-297
Author(s):  
Steve Kalloger ◽  
Joanna Karasinska ◽  
Hui-li Wong ◽  
Daniel John Renouf ◽  
David F. Schaeffer
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Advanced Disease ◽  
A Priori ◽  
Predictive Ability ◽  
Ductal Adenocarcinoma ◽  
Secreted Protein ◽  
Kaplan Meier ◽  
Observation Methods ◽  
Sparc Expression ◽  
Disease Specific

297 Background: Secreted Protein, Acid, Cysteine-Rich (SPARC) has recently been postulated as a therapeutic target in pancreatic ductal adenocarcinoma (PDAC). The clinical trial findings investigating SPARC expression and nab-paclitaxel sensitivity have been discordant. This study aims to develop an integrated component based approach to the quantification of SPARC in PDAC to identify discrete predictive subgroups in a cohort of resected patients treated with an gemcitabine (GEM) or subjected to post-surgical observation. Methods: Immunohistochemical quantification of SPARC was performed on the epithelial and stromal compartments of resected PDAC on 219 patient samples on a tissue-microarray. The staining was assessed by the generation of H-Scores. The resultant scores were subjected to unsupervised hierarchical clustering. The maximum number of clusters was determined through an a priori decision that no cluster could be composed of less than 15% of the cohort. Univariable disease specific survival (DSS) analysis was performed with the Kaplan-Meier method to examine the cluster specific survival profiles with regard to gemcitabine sensitivity. Results: Mean age was 67 [38-88] with 56% being male. Most of the cohort had advanced disease with pT3 = 95% and pN1 = 72%. Lymphovascular and perinueural invasion were found in 58% and 93% of the cohort respectively. Clusters ranging in size from 35 to 76 cases were derived and represented the four-biomarker combinations of Low/Low, Low/High, High/High, and High/Low for the epithelial and stromal components respectively. None of the clinico-pathologic variables were significantly enriched in the clusters. Assessment of the predictive ability of the 4 clusters demonstrated that only one cluster (High/High) representing 76 (35%) patients in this cohort was sensitive to adjuvant GEM (p = 0.0067). Conclusions: This study shows that there is enhanced value in a combinatorial approach to the examination of SPARC in the stromal and epithelial components of PDAC where we have discovered that co-expression in both the epithelial and stromal components is significantly associated with sensitivity to adjuvant GEM.

Localization of Secreted Protein Acidic and Rich in Cysteine (SPARC) Expression in the Rat Eye

1999 ◽  
Vol 40 (4) ◽  
pp. 295-303 ◽  
Author(s):  
Richard E. Gilbert ◽  
Alison J. Cox ◽  
Darren J. Kelly ◽  
Jennifer L. Wilkinson-Berka ◽  
E. Helene Sage ◽  
...  
Keyword(s):  
Secreted Protein ◽  
Sparc Expression

scholarly journals Secreted Protein Acidic and Rich in Cysteine as a Molecular Physiological and Pathological Biomarker

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1689
Author(s):  
Abdelaziz Ghanemi ◽  
Mayumi Yoshioka ◽  
Jonny St-Amand
Keyword(s):  
Serum Levels ◽  
Secreted Protein ◽  
Biological Functions ◽  
Mechanistic Studies ◽  
Pharmacological Agents ◽  
Disease Prognosis ◽  
Its Gene ◽  
Potential Applications ◽  
Cancer And Inflammation ◽  
Sparc Expression

Secreted protein acidic and rich in cysteine (SPARC) is expressed in diverse tissues and plays roles in various biological functions and processes. Increased serum levels of SPARC or its gene overexpression have been reported following numerous physiological and pathological changes including injuries, exercise, regeneration, obesity, cancer, and inflammation. Such expression pattern interrelation between these biological changes and the SPARC expression/secretion points to it as a biomarker. This property could lead to a variety of potential applications ranging from mechanistic studies and animal model validation to the clinical and therapeutic evaluation of both disease prognosis and pharmacological agents.

scholarly journals Measuring Exercise-Induced Secreted Protein Acidic and Rich in Cysteine Expression as a Molecular Tool to Optimize Personalized Medicine

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1832
Author(s):  
Abdelaziz Ghanemi ◽  
Mayumi Yoshioka ◽  
Jonny St-Amand
Keyword(s):  
Gene Expression ◽  
Personalized Medicine ◽  
Research Field ◽  
Secreted Protein ◽  
Molecular Tool ◽  
Pathological Conditions ◽  
Field Measuring ◽  
Exercise Induced ◽  
Sparc Expression ◽  
Selection Of

The numerous exercise benefits for health as well as applications for diseases has lead to exercise being prescribed in many pathological conditions. Secreted protein acidic and rich in cysteine (SPARC) gene expression is stimulated by exercise and SPARC has been suggested as a molecular mediator of exercise. Therefore, we suggest using this property for personalized medicine. This can be achieved by prescribing the exercise with a pattern (duration, intensity, etc.) that corresponds to the optimum SPARC/Sparc expression. We expect this approach to optimize the exercise therapy in both the preventive and curative contexts. In the research field, measuring exercise -dependent expression of Sparc would represent a molecular tool to further optimize the selection of exercise animal models as well.

scholarly journals Impact of Secreted Protein Acidic and Rich in Cysteine (SPARC) Expression on Prognosis After Surgical Resection for Biliary Carcinoma

2017 ◽  
Vol 21 (6) ◽  
pp. 990-999 ◽  
Author(s):  
Kazuhiro Toyota ◽  
Yoshiaki Murakami ◽  
Naru Kondo ◽  
Kenichiro Uemura ◽  
Naoya Nakagawa ◽  
...  
Keyword(s):  
Surgical Resection ◽  
Secreted Protein ◽  
Biliary Carcinoma ◽  
Sparc Expression
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