Comprehensive Laboratory Evaluation of a Specific Lateral Flow Assay for the Presumptive Identification of Abrin in Suspicious White Powders and Environmental Samples

2014 ◽  
Vol 12 (1) ◽  
pp. 49-62 ◽  
Author(s):  
Jason G. Ramage ◽  
Kristin Willner Prentice ◽  
Stephen A. Morse ◽  
Andrew J. Carter ◽  
Shomik Datta ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Laboratory Evaluation ◽  
Presumptive Identification

scholarly journals Comprehensive Laboratory Evaluation of a Highly Specific Lateral Flow Assay for the Presumptive Identification ofBacillus anthracisSpores in Suspicious White Powders and Environmental Samples

2016 ◽  
Vol 14 (5) ◽  
pp. 351-365 ◽  
Author(s):  
Jason G. Ramage ◽  
Kristin W. Prentice ◽  
Lindsay DePalma ◽  
Kodumudi S. Venkateswaran ◽  
Sruti Chivukula ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Laboratory Evaluation ◽  
Presumptive Identification

scholarly journals Comprehensive Laboratory Evaluation of a Specific Lateral Flow Assay for the Presumptive Identification of Francisella tularensis in Suspicious White Powders and Aerosol Samples

2020 ◽  
Vol 18 (2) ◽  
pp. 83-95
Author(s):  
Segaran P. Pillai ◽  
Lindsay DePalma ◽  
Kristin W. Prentice ◽  
Jason G. Ramage ◽  
Carol Chapman ◽  
...  
Keyword(s):  
Francisella Tularensis ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Laboratory Evaluation ◽  
Presumptive Identification

scholarly journals Comprehensive Laboratory Evaluation of a Lateral Flow Assay for the Detection of Yersinia pestis

2019 ◽  
Vol 17 (6) ◽  
pp. 439-453 ◽  
Author(s):  
Kristin W. Prentice ◽  
Lindsay DePalma ◽  
Jason G. Ramage ◽  
Jawad Sarwar ◽  
Nishanth Parameswaran ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Laboratory Evaluation

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM).The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated at a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM). The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

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