Gene Expression Alteration of Sperm-Associated Antigens in Human Cryopreserved Sperm

2021 ◽  
Author(s):  
Samaneh Faraji ◽  
Leila Rashki Ghaleno ◽  
Mohsen Sharafi ◽  
Maryam Hezavehei ◽  
Mehdi Totonchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Alteration ◽  
Cryopreserved Sperm

scholarly journals Transcriptome-wide gene expression alteration of sloe bug (Insecta: Hemiptera: Dolycoris baccarum) to divergent-altitude environments

2019 ◽  
Author(s):  
Qi-Lin Zhang ◽  
Ming-Long Yuan ◽  
Xian-Yu Deng ◽  
Jun Guo ◽  
Feng Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Alteration

scholarly journals Systematic Analysis of Gene Expression Alteration and Co-Expression Network of Eukaryotic Initiation Factor 4A-3 in Cancer

2018 ◽  
Vol 9 (24) ◽  
pp. 4568-4577 ◽  
Author(s):  
Yan Lin ◽  
Jinyan Zhang ◽  
Junying Cai ◽  
Rong Liang ◽  
Guoying Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Initiation Factor ◽  
Eukaryotic Initiation Factor ◽  
Systematic Analysis ◽  
Gene Expression Alteration

scholarly journals Cis-regulatory somatic mutations and gene-expression alteration in B-cell lymphomas

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Anthony Mathelier ◽  
Calvin Lefebvre ◽  
Allen W Zhang ◽  
David J Arenillas ◽  
Jiarui Ding ◽  
...  
Keyword(s):  
Gene Expression ◽  
B Cell ◽  
Somatic Mutations ◽  
B Cell Lymphomas ◽  
Gene Expression Alteration

scholarly journals Correction to: New insights in gene expression alteration as effect of doxorubicin drug resistance in triple negative breast cancer cells

2020 ◽  
Vol 39 (1) ◽  
Author(s):  
Cristina Alexandra Ciocan-Cartita ◽  
Ancuta Jurj ◽  
Oana Zanoaga ◽  
Roxana Cojocneanu ◽  
Laura-Ancuta Pop ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Drug Resistance ◽  
Cancer Cells ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cells ◽  
Triple Negative ◽  
Gene Expression Alteration

An amendment to this paper has been published and can be accessed via the original article.

scholarly journals Microarray Analysis of Gene Expression Alteration in Human Middle Ear Epithelial Cells Induced by Asian Sand Dust

2015 ◽  
Vol 8 (4) ◽  
pp. 345 ◽  
Author(s):  
Yoon Young Go ◽  
Moo Kyun Park ◽  
Jee Young Kwon ◽  
Young Rok Seo ◽  
Sung-Won Chae ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Microarray Analysis ◽  
Middle Ear ◽  
Gene Expression Alteration ◽  
Human Middle Ear ◽  
Asian Sand Dust ◽  
Sand Dust

20 Development and Oct4/Cdx2 gene expression of Puma concolor, Leopardus geoffroyi, and Panthera onca hybrid embryos produced using domestic cat oocytes

2021 ◽  
Vol 33 (2) ◽  
pp. 117
Author(s):  
M. D. Rodriguez ◽  
A. Sestelo ◽  
C. Buemo ◽  
L. D. Ratner ◽  
R. Fernandez-Martin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sperm Motility ◽  
Assisted Reproductive Technologies ◽  
Puma Concolor ◽  
Quantitative Polymerase Chain Reaction ◽  
Expression Patterns ◽  
Reproductive Technologies ◽  
Domestic Cat ◽  
Panthera Onca ◽  
Cryopreserved Sperm

The development of assisted reproductive technologies (ARTs) in Felidae species has been proposed to guarantee the genetic viability of species that are on the verge of extinction. Investigation of sperm cryopreservation and IVF have been studied in several Felidae species, but the lack of viable homologous oocytes makes assessment of sperm fertilization capacity after cryopreservation difficult. We propose to evaluate the capability of Puma concolor (Pc), Leopardus geoffroyi (Lg), and Panthera onca (Po) cryopreserved sperm to induce domestic cat oocyte fertilization and subsequent pre-implantation embryo development. Additionally, we evaluated blastocyst quality by analysing the gene expression patterns of pluripotency (Oct4) and differentiation (Cdx2) related markers using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). After ovariectomy, domestic cat (Felis catus, Fc) cumulus–oocyte complexes were matured invitro at 38.5°C in a humidified atmosphere of 6.5% CO2 and 21% O2 for 22h. Cryopreserved sperm from Pc, Lg, and Po were used for heterologous IVF. The sperm was frozen using Tes-Tris–based diluent (TEST), and survival, sperm motility, and acrosome integrity were evaluated. The quality post-thaw was satisfactory. The survival was high, sperm motility (65%) did not decrease during incubation post-thaw, and sperm with intact acrosome was 62% for all species. Briefly, straws were thawed by exposing them to air for 10s and then immersing in a 37°C water bath for 30s. The contents of the straws were poured into prewarmed sterile 1.5-mL microtubes. The sperm suspension was diluted (1:3 vol/vol) by the slow (drop by drop) addition of a modified Tyrode’s solution (Pope et al. 2006 Methods Molec. Biol. 254, 227-244). For IVF, invitro matured oocytes (n=635) were co-incubated with 0.5×105 mL−1 motile spermatozoa at 38.5°C in a humidified atmosphere of 6.5% CO2 and 21% O2 for 18 to 20h. Presumptive zygotes were cultured invitro in 50-µL drops of modified Tyrode’s medium in a humidified gas mixture of 5% CO2, 5% O2, and 90% N2 at 38.5°C. Cleavage was determined 48h post-fertilization and blastocyst stage was evaluated at Day 8 and stored in RNA-Later at −20°C until RT-qPCR analysis. Data were analysed by Fisher’s exact test using GraphPad Prism 6.0 (GraphPad Inc.), and differences were considered significant at P<0.05. We observed significantly lower cleavage rates of Po hybrid (hPo) embryos compared with hPc, hLg, and control Fc embryos (41% vs. 57%, 68%, and 58%, respectively). Additionally, we found that the blastocyst rate was higher using Lg sperm compared with sperm of other groups [hLg (50%) vs. hPc (29%), hPo (21%), and Fc (27%)].

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