Effect of Quercetin on Oxidative Stress, Mitochondrial Activity, and Quality of Indian Red Jungle Fowl (Gallus gallus murghi) Sperm

2020 ◽  
Vol 18 (4) ◽  
pp. 311-320
Author(s):  
Bushra Allah Rakha ◽  
Qurrat-ul-Ain ◽  
Muhammad S. Ansari ◽  
Shamim Akhter ◽  
Ali Akhter ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gallus Gallus ◽  
Mitochondrial Activity ◽  
Red Jungle Fowl

Impact of Ejaculate Frequencies on the Quality of Red Jungle Fowl (Gallus gallus murghi) semen

2015 ◽  
Vol 8 (2) ◽  
pp. 109-112 ◽  
Author(s):  
Bushra Allah Rakha ◽  
Iftikhar Hussain ◽  
Asma-ul-Husna ◽  
Muhammad Fahim Malik ◽  
Shamim Akhter ◽  
...  
Keyword(s):  
Gallus Gallus ◽  
Red Jungle Fowl

scholarly journals Metformin Improves Quality of Post-Thaw Canine Semen

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 287 ◽  
Author(s):  
Jérémy Grandhaye ◽  
Agnieszka Partyka ◽  
Zuzanna Ligocka ◽  
Agata Dudek ◽  
Wojciech Niżański ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Fertilization Rate ◽  
Mitochondrial Activity ◽  
Assisted Reproductive Technique ◽  
Dna Damages ◽  
Semen Extender ◽  
Metabolic Activities

Sperm cryopreservation is an assisted reproductive technique routinely used in canine species for genetic conservation. However, during cryopreservation, the DNA damages are still elevated, limiting the fertilization rate. The present study was conducted to evaluate whether supplementation of canine semen extender with a molecule limiting the metabolic activities can improve the quality of frozen-thawed canine spermatozoa. We used metformin, known to limit the mitochondrial respiratory and limit the oxidative stress. Before and during the freezing procedure, metformin (50µM and 500µM) has been added to the extender. After thawing, sperm exposed to metformin conserved the same viability without alteration in the membrane integrity or acrosome reaction. Interestingly, 50µM metformin improved the sperm motility in comparison to the control, subsequently increasing mitochondrial activity and NAD+ content. In addition, the oxidative stress level was reduced in sperm treated with metformin improving the sperm quality as measured by a different molecular marker. In conclusion, we have shown that metformin is able to improve the quality of frozen-thawed dog semen when it is used during the cryopreservative procedure.

Effect of glutathione on pre and post-freezing sperm quality of Indian red jungle fowl (Gallus gallus murghi)

2021 ◽  
Author(s):  
M.S. Ansari ◽  
B.A. Rakha ◽  
S. Akhter ◽  
A. Akhter ◽  
E. Blesbois ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Gallus Gallus ◽  
Red Jungle Fowl

60 Quercetin protects bovine pre-implantation embryos against oxidative stress via activation of Nrf2 signaling pathway

2019 ◽  
Vol 31 (1) ◽  
pp. 155
Author(s):  
O. Khadrawy ◽  
S. Gebremedhn ◽  
D. Salilew-Wondim ◽  
F. Rings ◽  
C. Neuhoff ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Embryo Culture ◽  
Protein Level ◽  
Culture Media ◽  
Mitochondrial Activity ◽  
Oxygen Level ◽  
Antioxidant Genes ◽  
Total Rna ◽  
Intracellular Ros

High atmospheric oxygen level during in vitro embryo culture system is reported to induce oxidative stress, leading to an increased intracellular reactive oxygen species (ROS) accumulation and lower the quality and development of the embryos. The Nrf2 is a redox sensitive transcription factor, which regulates the antioxidant machinery against oxidative stress in bovine pre-implantation embryos (Amin et al. 2014Mol. Reprod. Dev. 81, 497-513). Quercetin is a plant-derived flavonoid found in fruits and vegetables with antioxidant, anti-inflammatory, and anti-apoptotic properties. The effect of these antioxidants on the development and quality of bovine pre-implantation embryos with respect to the activation of the Nrf2 signalling pathways is not yet discovered. Here, we aimed to investigate the effect of quercetin supplementation on the activation of the Nrf2 signalling pathway and the subsequent impact on the quality of bovine embryos. For this, presumptive bovine zygotes were cultured in SOFaa embryo culture media supplemented with or without 10 µM quercetin (482 and 485 zygotes, respectively) and incubated under high oxygen level (20%) conditions. Day 7 and Day 8 blastocysts were analysed for the accumulation intracellular ROS and mitochondrial activity using H2DCFDA and MitoTracker® Red, respectively. Thereafter, blastocysts from both groups were subjected to total RNA isolation using Norgen total RNA purification plus kit (BioTek, Winooski, VT, USA). The relative abundance of Nrf2 and its downstream antioxidant genes (NQO1, PRDX1, SOD1, and CAT) was quantified using quantitative PCR. Moreover, the protein level of Nrf2 was detected using immunofluorescence staining. Data from 4 independent biological replicates were statistically analysed using a 2-tailed Student’s t-test. A significant difference in the mean value among treatments was determined at P=0.05. Results showed that quercetin supplementation in embryo culture medium significantly increased the blastocyst total cell number both at Day 7 (125±7.98 v. 100±3.74; P=0.007) and Day 8 (128±4.49 v. 95±3.15; P<0.0001). However, no difference was observed in the quercetin-treated group compared with control with respect to the cleavage rate (85.89±1.11v. 83.86±1.27; P=0.2) as well as the blastocyst rate at Day 7 (24.57±4.94 v. 27.23±1.68; P=0.64) and Day 8 (41.17±3.70 v. 38.87±0.85; P=0.58). Interestingly, quercetin-supplemented blastocysts showed a significant reduction in intracellular ROS level and increment in the mitochondrial activity compared with untreated counterparts. Supplementation of quercetin activated the Nrf2 transcriptional factor both at the mRNA and protein level, which subsequently activated the transcription of the aforementioned downstream antioxidant genes. In conclusion, supplementation of quercetin to embryo culture media protects embryos against oxidative stress by activating the Nrf2-mediated oxidative stress response mechanism, which leads to reduced accumulation of ROS and elevated mitochondrial activity.

scholarly journals Curcumin offers antioxidant protection to cryopreserved bovine semen

2018 ◽  
Vol 63 (No. 7) ◽  
pp. 247-255 ◽  
Author(s):  
E. Tvrdá ◽  
H. Greifová ◽  
A. Mackovich ◽  
F. Hashim ◽  
N. Lukáč
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Structural Integrity ◽  
Mitochondrial Activity ◽  
Stress Markers ◽  
Male Gametes ◽  
Bovine Spermatozoa ◽  
Oxidative Damage To Proteins ◽  
Antioxidant Molecule

Evidence shows that oxidative stress associated with sperm cryopreservation may lead to a significant decrease of the structural integrity and functional activity of male gametes. Curcumin (CUR) has become a substance of scientific interest for its free radical-quenching abilities, which could enhance the post-thaw quality of male gametes. This study assessed the effects of CUR on the post-thaw vitality and selected oxidative stress markers of bovine spermatozoa. Thirty ejaculates collected from 10 breeding bulls were divided into two aliquots and cryopreserved in the absence (control) or presence of CUR (50 μmol/l). Immediately before use, the control or experimental straws were thawed at 37°C for 20 s. CUR administration led to a significantly higher preservation of spermatozoa motion (P < 0.001) as well as membrane (P < 0.05) and acrosomal (P < 0.01) integrity in comparison with the control. Moreover, spermatozoa exposed to CUR exhibited a significantly higher mitochondrial activity (P < 0.001). Significantly decreased amounts of reactive oxygen species (P < 0.01) and superoxide (P < 0.001) were detected following CUR supplementation. Finally, a significant decrease of oxidative damage to proteins (P < 0.01), lipids (P < 0.001), and DNA (P < 0.05) was recorded in samples to which CUR was administered in comparison to the control. In this study, CUR proved to act as an efficient antioxidant molecule offering protection to male gametes against oxidative damage during cryopreservation.

93 EFFECT OF ANTIOXIDANTS SUPPLEMENTATION ON THE QUALITY OF CRYOPRESERVED SPERM OF DOGS

2011 ◽  
Vol 23 (1) ◽  
pp. 152
Author(s):  
C. A. B. Sobrinho ◽  
M. Nichi ◽  
P. A. A. Góes ◽  
A. Dalmazzo ◽  
S. E. Crusco ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Vitamin E ◽  
Reactive Oxygen ◽  
The Other ◽  
Mitochondrial Activity ◽  
Enzymatic Antioxidants ◽  
Oxygen Species ◽  
Intact Membrane

One of the main causes of poor quality of frozen–thawed dog sperm is oxidative stress (i.e. higher production of reactive oxygen species not compensated by improved antioxidant protection). This event is known to impair sperm functionality by attacking plasma membrane, acrosome, mitochondria, and DNA. Spermatozoa are particularly susceptible the oxidative stress, mainly due to the reduced cytoplasm and the high content of polyunsaturated fatty acids (PUFA) in the membrane, which allows the spermatozoa to be motile and confers a higher resistance against the damages caused by cryopreservation, but makes the sperm more susceptible to the attack of the reactive oxygen species (ROS). The present study aimed to evaluate the effects of antioxidant supplementation on semen extender (Tris-egg yolk-citrate-glicerol) with glutathione (GSH) and vitamin E on the quality of cryopreserved dog sperm. Ejaculates of 12 dogs were divided in pools of 3 ejaculates with at least 70% of motility. Each pool was diluted with 7 different extenders for treatment groups as follows: control, vitamin E (1, 5, and 10 mM), and reduced glutathione (GSH; 1, 5, and 10 mM) and submitted to cryopreservation. Samples were thawed (37°C/30′) and evaluated for motility, vigor, percentage of sperm showing intact membrane (eosin/nigrosin), and acrosome (simple stain fast-green and bengal rose), mitochondrial activity (3–3′-diaminobenzidine-DAB), and sperm susceptibility to oxidative stress (TBARS). Statistical analyses were performed using the SAS system for Windows (SAS Institute Inc., Cary, NC, USA; least significant differences test and Spearman correlation; P < 0.05). Samples treated with 1 mM of GSH showed a higher percentage of sperm with intact membrane when compared with the control (11.21 ± 2.84 and 6.21 ± 1.16%, respectively; P < 0.05). On the other hand, treatment with 5 mM of GSH showed better results regarding mitochondrial activity. Vitamin E supplementation also played a protective role on mitochondrial activity; samples treated with 1 mM showed a lower percentage of DAB III sperm (cells with severely compromised mitochondrial activity) when compared with the control group (5.61 ± 0.7 and 8.62 ± 1.05%, respectively; P < 0.05). Both vitamin E and GSH are important non-enzymatic antioxidants responsible for the destruction of the hydroxyl radical. Despite the positive influence of these antioxidants on mitochondrial status, no effect was found on the other variables studied. These results indicate that the action of both antioxidants in dog sperm would be mainly intracellular. Furthermore, other ROS could be responsible for the other damages caused by cryopreservation on the other sperm functionalities (i.e. membrane, acrosome, DNA, oxidative status). Therefore, the use of a combination of enzymatic and non-enzymatic antioxidants could be an alternative to overcome the deleterious influence of oxidative stress in cryopreserved semen of dogs. The authors thank the Brazilian army for the dogs used in this study.

scholarly journals Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karina Cañón-Beltrán ◽  
Yulia N. Cajas ◽  
Serafín Peréz-Cerezales ◽  
Claudia L. V. Leal ◽  
Ekaitz Agirregoitia ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Mitochondrial Activity ◽  
Bovine Embryos ◽  
Akt Signaling Pathway ◽  
Cell Stage ◽  
Development In Vitro

AbstractIn vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + 5% FCS (Control) or supplemented with 5, 10 or 25 µM nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (CDMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJEGA) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 ± 1.0%) and Nob10 (44.4 ± 1.3%) for MNEGA phase and in Nob10 (61.0 ± 0.8%) for MJEGA phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPARα transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway.

scholarly journals Effect of Oxidative Stress on Physicochemical Quality of Taiwanese Seagrape (Caulerpa lentillifera) with the Application of Alternating Current Electric Field (ACEF) during Post-Harvest Storage

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1011
Author(s):  
Andi Syahrullah Sulaimana ◽  
Chao-Kai Chang ◽  
Chih-Yao Hou ◽  
Bara Yudhistira ◽  
Fuangfah Punthi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Electric Field ◽  
Alternating Current ◽  
Chlorophyll Degradation ◽  
Physicochemical Quality ◽  
Post Harvest ◽  
Caulerpa Lentillifera ◽  
Different Seasons ◽  
Current Electric Field

This study aims to determine the physicochemical quality of seagrape (Caulerpa lentillifera) as a freshness label for products cultivated in different seasons. The applied post-harvest storage experiments compared between, within and without seawater that led to oxidative stress conditions. Water content, malondialdehyde (MDA) compound, total phenolic content (TPC), and chlorophyll content were observed at 0, 3, 6, and 9 days of storage. The storage without seawater showed sharper quality reductions by reaching 20–40% of water loss, 70–90% of MDA production, 15–25% of TPC reduction, and 40–60% of total chlorophyll degradation. The storage within seawater showed lower quality reductions due to the specific growth rates still reaching 5–10%. This study found that the greater the physicochemical quality, the slower the decomposition rates of the stored seagrape during storage. Therefore, the seagrapes’ obvious discoloration occurred earlier in winter, followed by summer and spring. Kinetics of chlorophyll degradation on seagrape in different seasons meet different order-reactions during storage. Furthermore, alternating current electric field (ACEF) treatment with 125 kV/m of intensity for 60 min can lower the spring seagrapes’ physicochemical quality by reaching 10–30% of inhibition, resulting in the shelf-life extension for up to 12 days of post-harvest storage.

Sign in / Sign up

Export Citation Format

Share Document