Histological Assessment of Tumor Tissue Samples via the Mirror Image Method

2015 ◽  
Vol 13 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Haixin Li ◽  
Yan Guo ◽  
Baocun Sun ◽  
Kexin Chen
Keyword(s):  
Tumor Tissue ◽  
Mirror Image ◽  
Image Method ◽  
Tissue Samples ◽  
Histological Assessment

Is the Mirror Image Method Really Useful in Tumor Tissue Bank Quality Control?

2019 ◽  
Vol 17 (6) ◽  
pp. 539-545
Author(s):  
Oriane Dudez ◽  
Véronique Dalstein ◽  
Lukshe Kanagaratnam ◽  
Saviz Nasri ◽  
Christelle Coquelet ◽  
...  
Keyword(s):  
Quality Control ◽  
Tumor Tissue ◽  
Tissue Bank ◽  
Mirror Image ◽  
Image Method

A Novel Method for Microsatellite Instability Detection by Liquid Biopsy Based on Next- Generation Sequencing

2020 ◽  
Vol 15 ◽  
Author(s):  
Zheng Jiang ◽  
Hui Liu ◽  
Siwen Zhang ◽  
Jia Liu ◽  
Weitao Wang ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Microsatellite Instability ◽  
Liquid Biopsy ◽  
Tumor Tissue ◽  
High Sensitivity ◽  
Next Generation ◽  
Tissue Samples ◽  
Next Generation Sequencing Technology ◽  
Medication Selection ◽  
Generation Sequencing

Background: Microsatellite instability (MSI) is a prognostic biomarker used to guide medication selection in multiple cancers, such as colorectal cancer. Traditional PCR with capillary electrophoresis and next-generation sequencing using paired tumor tissue and leukocyte samples are the main approaches for MSI detection due to their high sensitivity and specificity. Currently, patient tissue samples are obtained through puncture or surgery, which causes injury and risk of concurrent disease, further illustrating the need for MSI detection by liquid biopsy. Methods: We propose an analytic method using paired plasma/leukocyte samples and MSI detection using next-generation sequencing technology. Based on the theoretical progress of oncogenesis, we hypothesized that the microsatellite site length in plasma equals the combination of the distribution of tumor tissue and leukocytes. Thus, we defined a window-judgement method to identify whether biomarkers were stable. Results: Compared to traditional PCR as the standard, we evaluated three methods in 20 samples (MSI-H:3/MSS:17): peak shifting method using tissue vs. leukocytes, peak shifting method using plasma vs. leukocytes, and our method using plasma vs. leukocytes. Compared to traditional PCR, we observed a sensitivity of 100%, 0%, and 100%, and a specificity of 100.00%, 94.12%, and 88.24%, respectively. Conclusion: Our method has the advantage of possibly detecting MSI in a liquid biopsy and provides a novel direction for future studies to increase the specificity of the method.

scholarly journals Combining tissue and circulating tumor DNA increases the detection rate of a CTNNB1 mutation in hepatocellular carcinoma

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Stine Karlsen Oversoe ◽  
Michelle Simone Clement ◽  
Britta Weber ◽  
Henning Grønbæk ◽  
Stephen Jacques Hamilton-Dutoit ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Mutation Rate ◽  
Detection Rate ◽  
Tumor Tissue ◽  
Circulating Tumor Dna ◽  
Treatment Modalities ◽  
Advanced Hepatocellular Carcinoma ◽  
Tissue Samples ◽  
The Impact ◽  
Tumor Dna

Abstract Background and aims Studies suggest that mutations in the CTNNB1 gene are predictive of response to immunotherapy, an emerging therapy for advanced hepatocellular carcinoma (HCC). Analysis of circulating tumor DNA (ctDNA) offers the possibility of serial non-invasive mutational profiling of tumors. Combining tumor tissue and ctDNA analysis may increase the detection rate of mutations. This study aimed to evaluate the frequency of the CTNNB1 p.T41A mutation in ctDNA and tumor samples from HCC patients and to evaluate the concordance rates between plasma and tissue. We further evaluated changes in ctDNA after various HCC treatment modalities and the impact of the CTNNB1 p.T41A mutation on the clinical course of HCC. Methods We used droplet digital PCR to analyze plasma from 95 patients and the corresponding tumor samples from 37 patients during 3 years follow up. Results In tumor tissue samples, the mutation rate was 8.1% (3/37). In ctDNA from HCC patients, the CTNNB1 mutation rate was 9.5% (9/95) in the pre-treatment samples. Adding results from plasma analysis to the subgroup of patients with available tissue samples, the mutation detection rate increased to 13.5% (5/37). There was no difference in overall survival according to CTNNB1 mutational status. Serial testing of ctDNA suggested a possible clonal evolution of HCC or arising multicentric tumors with separate genetic profiles in individual patients. Conclusion Combining analysis of ctDNA and tumor tissue increased the detection rate of CTNNB1 mutation in HCC patients. A liquid biopsy approach may be useful in a tailored therapy of HCC.

Combination Resonance of a Magnet Over a High-TC Superconductor Excited Vertically

2001 ◽  
Author(s):  
Toshihiko Sugiura ◽  
Masayuki Kondo
Keyword(s):  
Magnetic Force ◽  
Vertical Direction ◽  
Vertical Motion ◽  
Mirror Image ◽  
Image Method ◽  
Combination Resonance ◽  
Nonlinear Functions ◽  
High Tc ◽  
High Tc Superconductor ◽  
Oscillation Modes

Abstract This research deals with nonlinear dynamics of a permanent magnet freely levitated above a high-Tc superconductor (HTSC) excited in the vertical direction. Magnetic force and torque can be evaluated analytically by the advanced mirror image method as nonlinear functions of both displacement and roll angle of the magnet. Equations of 3 d.o.f. motion show that the magnet has two oscillation modes due to linear coupling of the horizontal and roll motions. The both modes can be excited by nonlinear coupling with vertical motion when the superconductor is exited vertically in the neighborhood of the sum of the natural frequency of each mode. Frequency response of this combination resonance was numerically simulated. This resonance was also observed in experiments.

scholarly journals PATH-06. ASSESSMENT OF CIRCULATING TUMOR DNA IN CEREBROSPINAL FLUID BY WHOLE EXOME SEQUENCING TO DETECT GENOMIC ALTERATIONS OF GLIOBLASTOMA

2020 ◽  
Vol 22 (Supplement_2) ◽  
pp. ii165-ii165
Author(s):  
Hao Duan ◽  
Zhenqiang He ◽  
Zhenghe Chen ◽  
Yonggao Mou
Keyword(s):  
Cerebrospinal Fluid ◽  
Exome Sequencing ◽  
Whole Exome Sequencing ◽  
Tumor Tissue ◽  
Circulating Tumor Dna ◽  
Genomic Alterations ◽  
Tissue Samples ◽  
Whole Exome ◽  
Resected Tumor ◽  
Tumor Dna

Abstract Cerebrospinal fluid (CSF) has been demonstrated as a better source of circulating tumor DNA (ctDNA) than plasma for brain tumors. However, it is unclear whether whole exome sequencing (WES) is qualified for detection of ctDNA in CSF. The aim of this study was to determine if assessment of ctDNA in CSF by WES is a feasible approach to detect genomic alterations of glioblastoma. CSFs of ten glioblastoma patients were collected pre-operatively at the Department of Neurosurgery, Sun Yat-sen University Cancer Center. ctDNA in CSF and genome DNA in the resected tumor were extracted and subjected to WES. The identified glioblastoma-associated mutations from ctDNA in CSF and genome DNA in the resected tumor were compared. Due to the ctDNA in CSF was unqualified for exome sequencing for one patient, nine patients were included into the final analysis. More glioblastoma-associated mutations tended to be detected in CSF comparing with the corresponding tumor tissue samples (3.56±0.75 vs. 2.22±0.32, P=0.097), while the statistical significance was limited by the small sample size. The average mutation frequencies were similar in CSF and tumor tissue samples (74.12% ± 6.03% vs. 73.83% ± 5.95%, P = 0.924). The R132H mutation of isocitrate dehydrogenase 1 and the G34V mutation of H3F3A which had been reported in the pathological diagnoses were also detected from ctDNA in CSF by WES. Patients who received temozolomide chemotherapy previously or those whose tumor involved subventricular zone tended to harbor more mutations in their CSF. Assessment of ctDNA in CSF by WES is a feasible approach to detect genomic alterations of glioblastoma, which may provide useful information for the decision of treatment strategy.

Immunohistochemical testing of BRAF V600E status in 1,120 tumor tissue samples of patients with brain metastases

2011 ◽  
Vol 123 (2) ◽  
pp. 223-233 ◽  
Author(s):  
David Capper ◽  
Anna Sophie Berghoff ◽  
Manuel Magerle ◽  
Aysegül Ilhan ◽  
Adelheid Wöhrer ◽  
...  
Keyword(s):  
Brain Metastases ◽  
Tumor Tissue ◽  
Braf V600e ◽  
Tissue Samples ◽  
Immunohistochemical Testing

The level of cytoskeleton remodeling proteins in the blood serum and the expression of their mRNA in the tumor tissue in metastasis of the larynx and hypopharynx cancer

2021 ◽  
Author(s):  
Gelena Kakurina ◽  
Olga V Cheremisina ◽  
Elena E Sereda ◽  
Elena S Kolegova ◽  
Irina V Kondakova ◽  
...  
Keyword(s):  
Serum Level ◽  
Blood Serum ◽  
Mrna Expression ◽  
Tumor Tissue ◽  
Mrna Level ◽  
Actin Binding ◽  
Tissue Samples ◽  
Signaling Systems ◽  
Cytoskeleton Remodeling ◽  
The Relationship

Abstract Purpose: Actin-binding proteins (ABPs) and various signaling systems are involved in the metastasis of squamous cell carcinoma of the larynx and hypopharynx (SCCLH). The clinical significance of these proteins has not yet been determined. We analyzed the relationship between the mRNA level of cofilin 1 (CFL1), profilin 1 (PFN1), adenylyl cyclase-associated protein 1 (CAP1), SNAIL and RND3 with metastasis in the SCCLH tissue. The serum level of the listed ABPs was estimated and the relationship of them with the expression of the corresponding mRNA was carried out. Materials and methods: The expression level of ABPs mRNA was measured by real-time RT-PCR in paired tissue samples taken from 54 patients with SCCLH (T 1-4 N 0-1 M 0 ). Expression analysis was performed using the 2 - ΔΔ CT method. The level of ABPs in the blood serum was measured by ELISA. Statistical analysis was carried out using the SPSS Statistica 20.0 software package. Results: The mRNA expression of the studied genes in tumor tissue of patients with SCCLH T 1-3 N 0 M 0 and T 2-4 N 1-2 M 0 did not differ significantly. High expression of RND3 mRNA was accompanied by an increase in mRNA expression of all studied ABPs. In the blood serum of T 2-4 N 1-2 M 0 patients the level of PFN1 was significantly lower by 21% and the level of CAP1 was higher by 75% compared with the group of patients with T 1-4 N 0 M 0 stage. Conclusion: According to our data RND3 is involved in the regulation of molecular cascades SCCLH metastasis. PFN1 and CAP1 serum level can be a good classifier of metastases in patients with SCCLH.

scholarly journals Prognostic Impact of Tumor-Associated Macrophages on Survival Is Checkpoint Dependent in Classical Hodgkin Lymphoma

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 877 ◽  
Author(s):  
Kristiina Karihtala ◽  
Suvi-Katri Leivonen ◽  
Oscar Brück ◽  
Marja-Liisa Karjalainen-Lindsberg ◽  
Satu Mustjoki ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Digital Image Analysis ◽  
Tumor Tissue ◽  
Immune Escape ◽  
Clinical Impact ◽  
Prognostic Impact ◽  
Classical Hodgkin Lymphoma ◽  
Tissue Samples ◽  
Tumor Associated Macrophage ◽  
Poor Outcomes

Tumor microenvironment and immune escape affect pathogenesis and survival in classical Hodgkin lymphoma (cHL). While tumor-associated macrophage (TAM) content has been associated with poor outcomes, macrophage-derived determinants with clinical impact have remained undefined. Here, we have used multiplex immunohistochemistry and digital image analysis to characterize TAM immunophenotypes with regard to expression of checkpoint molecules programmed cell death ligand 1 (PD-L1) and indoleamine 2,3-dioxygenase 1 (IDO-1) from the diagnostic tumor tissue samples of 130 cHL patients, and correlated the findings with clinical characteristics and survival. We show that a large proportion of TAMs express PD-L1 (CD68+, median 32%; M2 type CD163+, median 22%), whereas the proportion of TAMs expressing IDO-1 is lower (CD68+, median 5.5%; CD163+, median 1.4%). A high proportion of PD-L1 and IDO-1 expressing TAMs from all TAMs (CD68+), or from CD163+ TAMs, is associated with inferior outcome. In multivariate analysis with age and stage, high proportions of PD-L1+ and IDO-1+ TAMs remain independent prognostic factors for freedom from treatment failure (PD-L1+CD68+/CD68+, HR = 2.63, 95% CI 1.17–5.88, p = 0.019; IDO-1+CD68+/CD68+, HR = 2.48, 95% CI 1.03–5.95, p = 0.042). In contrast, proportions of PD-L1+ tumor cells, all TAMs or PD-L1− and IDO-1− TAMs are not associated with outcome. The findings implicate that adverse prognostic impact of TAMs is checkpoint-dependent in cHL.

The Research of the Electric Vehicle Wiring Harness Dynamic Crosstalk Based on Statistical Simulation Method

2014 ◽  
Vol 556-562 ◽  
pp. 1852-1855 ◽  
Author(s):  
Tian Hao Wang ◽  
Yin Han Gao ◽  
Kai Yu Yang ◽  
Jun Dong Zhang ◽  
Le Gao ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Simulation Method ◽  
Statistical Simulation ◽  
Mirror Image ◽  
Tube Placement ◽  
Image Method ◽  
Wiring Harness ◽  
Wire Harness ◽  
The Wire ◽  
Emc Design

In the actual situation, because of the shock of automobile in the movement process and the wire harness in undulating tube placement is random, leading to the relative position of the wire harness is not fixed, Thus lead to crosstalk value has certain dynamic range. In this paper, obtained the unit mutual inductance and mutual capacitance of wire with insulation layer using mirror image method, simulation and prediction of crosstalk dynamic interval by statistical simulation method, obtain harness near end crosstalk changes range within 3dB at a confidence level of 80%. This provides a reference for EMC design of automotive wiring harness.

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