scholarly journals Assessment of DNA Encapsulation, a New Room-Temperature DNA Storage Method

2014 ◽  
Vol 12 (3) ◽  
pp. 176-183 ◽  
Author(s):  
Dominique Clermont ◽  
Sylvain Santoni ◽  
Safa Saker ◽  
Maite Gomard ◽  
Eliane Gardais ◽  
...  
Keyword(s):  
Room Temperature ◽  
Storage Method ◽  
Dna Storage ◽  
Dna Encapsulation

Room temperature DNA storage with slide-mounted aphid specimens

2012 ◽  
Vol 6 (3) ◽  
pp. 447-451 ◽  
Author(s):  
GARY L. MILLER ◽  
ANDREW CARMICHAEL ◽  
COLIN FAVRET ◽  
SONJA J. SCHEFFER
Keyword(s):  
Room Temperature ◽  
Dna Storage

scholarly journals Assessing a novel room temperature DNA storage medium for forensic biological samples

2012 ◽  
Vol 6 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Steven B. Lee ◽  
Kimberly C. Clabaugh ◽  
Brie Silva ◽  
Kingsley O. Odigie ◽  
Michael D. Coble ◽  
...  
Keyword(s):  
Biological Samples ◽  
Room Temperature ◽  
Storage Medium ◽  
Dna Storage

scholarly journals INTERACTIVE EFFECTS OF SIZE, STORAGE METHOD AND DURATION ON QUALITY AND PHYSICAL CHARACTERISTICS OF TABLE EGGS

2021 ◽  
Vol 23 (1) ◽  
pp. 76-81
Author(s):  
S. O. KEMBI ◽  
A. A. OSOKOMAIYA
Keyword(s):  
Specific Gravity ◽  
Egg Size ◽  
Room Temperature ◽  
Egg Quality ◽  
Interactive Effects ◽  
Quality Changes ◽  
Quality Indices ◽  
Storage Method ◽  
Weight Losses ◽  
Long Storage

A total of 252 fresh table eggs sorted into standard, large and extra-large sizes were but.either refrigerated or held at room temperature and used to study their weekly weight losses (WL), specific gravity (SG), egg index (EI), yolk index (YI) and albumen height (AH) over a six week period. The results showed that among  room-stored eggs there were declines (P<0.01) AH, YI, SG and El but an increase in WL with increased egg size. Similar trends in response  to long storage were observed except that EI not affected (P>0.05). The depressive influences of long storage and larger sizes on egg quality observed in the room stored eggs diminished with refrigeration. The general patterns of quality deterioration show that,  irrespective of egg size, six-week refrigerated eggs had better quality indices than one-week room-stored eggs. There was no significant (P>0.05) influence of egg size on quality changes during storage Based on the result it was included  that in the absence of refrigeration and when eggs are sold in assorted sizes, table eggs should be consumed within 2 weeks of lay and larger eggs should be used first. 

Stable Plasma Sample Storage in Acetonitrile for Angiotensin and Aldosterone Analysis

2020 ◽  
Author(s):  
Xuefei Wei ◽  
Yanyang Wang ◽  
Wenbo Zhu ◽  
Jingjing Li ◽  
Lu Peng ◽  
...  
Keyword(s):  
Room Temperature ◽  
Plasma Sample ◽  
Sample Storage ◽  
Angiotensin I ◽  
Storage Method ◽  
Collaborative Studies ◽  
Stable Plasma

Abstract Background Angiotensin I, II (AI, AII) and aldosterone are unstable in plasma specimens at room temperature, making it difficult for collect samples for remote regions in centralized and collaborative studies. Here we introduce a stable storage method which do not require cold conditions.. Methods Acetonitrile was added to the plasma to 60%, and then the supernatants were kept at 4°C and room temperature for 0, 1, 2, 3, 10 and 30 days. AI, AII and aldosterone were extracted and analyzed by chemiluminescence immunoassays. Results AI, AII and aldosterone were well retained in the supernatant under this method. The intra- and inter-day CVs of this method were all below 10%. The levels of AI, AII and aldosterone by this method remained stable for 30 days at room temperature. Conclusion Addition of 60% acetonitrile in the plasma provides a stable storage method for clinical AI, AII and aldosterone.

scholarly journals POSTHARVEST QUALITY OF STRAWBERRY FRUITS GROWN IN CONV ENTIONAL SYSTEM

2020 ◽  
Vol 1 (1) ◽  
pp. 177-180
Author(s):  
Monica Sturzeanu
Keyword(s):  
Weight Loss ◽  
Open Field ◽  
Fruit Quality ◽  
Room Temperature ◽  
Fruit Weight ◽  
Postharvest Quality ◽  
Fruit Firmness ◽  
Storage Method ◽  
Before And After

The objective of the study was to evaluate some postharvest parameters of fruit quality at seven strawberry cvs., ('Alba', 'Clery', 'Coral', 'Magic', 'Premial', 'Queen Elisa', 'Record') grown at RIFG Pitești, Romania in open field, in the 2016-2018 period. The storage method included three days in normal refrigeration condition (2-4ºC) followed by one day at room temperature (22-24 ºC). The following parameters were determined before and after storage: fruit weight (g), fruit firmness (kgf/cm2), four color indicators (L*, a*, b*, ∆E) and percent of damaged fruits caused by pathogen. Statistically assured differences were recorded between cultivars. 'Premial' cv. proved the lowest fruit weight loss (0.27 g) and also the lowest fruit firmness loss (0.09 kgf/cm2) after storage. 'Alba' cv. had the highest percentage of healthy fruits after storage (86.4%). After four days storage both early cvs.were marketable.

scholarly journals Protocols for dry DNA storage and shipment at room temperature

2013 ◽  
Vol 13 (5) ◽  
pp. 890-898 ◽  
Author(s):  
Natalia V. Ivanova ◽  
Masha L. Kuzmina
Keyword(s):  
Room Temperature ◽  
Dna Storage

Changes in the quality of table eggs depending on storage method and time

2018 ◽  
Vol 14 (3) ◽  
pp. 37-45
Author(s):  
Magdalena Kopacz ◽  
Aleksandra Drażbo
Keyword(s):  
Room Temperature ◽  
Storage Time ◽  
Egg Quality ◽  
Shell Quality ◽  
Storage Method ◽  
Class A ◽  
Group I ◽  
Air Space ◽  
Egg Density

The aim of the research was to determine the changes occurring in table eggs depending on storage time at room temperature and in refrigeration conditions. The experimental material consisted of 75 eggs, which were randomly divided into five groups of 15. The first group (the control) comprised fresh eggs, which were tested on the day they were collected. The remaining 60 eggs were divided into two groups (30 eggs in each) and stored under different conditions for a period of 28 days. Eggs from the first group (I) were stored at room temperature, which was about 20°C with 50% humidity. Eggs from the second group (II) were placed in cold storage at 4°C and about 30% humidity. After 14 and 28 days of storage, the physicochemical properties of the eggs were analysed: the height of the air space, egg density, egg weight loss, albumen and shell quality, and yolk colour. The results indicate that the storage method had a significant (P≤0.05) effect on most of the egg quality traits analysed. Eggs stored at 4°C were of good quality and were classified as EXTRA class eggs even after 28 days. Eggs stored at 20°C were eliminated as Class A eggs as early as day 14, and on day 28 their quality disqualified them as fit for consumption. To sum up, food eggs should be refrigerated before being sold to the consumer to prevent premature ageing and thus deterioration of quality.

Dependence of Intergranular Fracture on Boundary Topography and Cohesion

1973 ◽  
Vol 31 ◽  
pp. 150-151
Author(s):  
J. E. Doherty ◽  
A. F. Giamei ◽  
B. H. Kear ◽  
C. W. Steinke
Keyword(s):  
Grain Boundary ◽  
Room Temperature ◽  
Nickel Base Superalloy ◽  
Boundary Shear ◽  
Room Temperature Ductility ◽  
Solid Solution Matrix ◽  
Nickel Base ◽  
Solution Matrix ◽  
Different Levels ◽  
Base Superalloy

Recently we have been investigating a class of nickel-base superalloys which possess substantial room temperature ductility. This improvement in ductility is directly related to improvements in grain boundary strength due to increased boundary cohesion through control of detrimental impurities and improved boundary shear strength by controlled grain boundary micros true tures.For these investigations an experimental nickel-base superalloy was doped with different levels of sulphur impurity. The micros tructure after a heat treatment of 1360°C for 2 hr, 1200°C for 16 hr consists of coherent precipitates of γ’ Ni3(Al,X) in a nickel solid solution matrix.

Hepatocyte Alterations in Guinea Pigs FED Polychlorinated Biphenyls (PCBs), Dibenzodioxins (PCDD), AND DIBENZOFURANS (PCDF)

1982 ◽  
Vol 40 ◽  
pp. 56-57
Author(s):  
J. N. Turner ◽  
D. N. Collins
Keyword(s):  
Guinea Pigs ◽  
Room Temperature ◽  
Dose Group ◽  
Methyl Cellulose ◽  
Uranyl Acetate ◽  
Target Organ ◽  
Office Building ◽  
Lead Citrate ◽  
Control Groups ◽  
Cooling Fluid

A fire involving an electric service transformer and its cooling fluid, a mixture of PCBs and chlorinated benzenes, contaminated an office building with a fine soot. Chemical analysis showed PCDDs and PCDFs including the highly toxic tetra isomers. Guinea pigs were chosen as an experimental animal to test the soot's toxicity because of their sensitivity to these compounds, and the liver was examined because it is a target organ. The soot was suspended in 0.75% methyl cellulose and administered in a single dose by gavage at levels of 1,10,100, and 500mgm soot/kgm body weight. Each dose group was composed of 6 males and 6 females. Control groups included 12 (6 male, 6 female) animals fed activated carbon in methyl cellulose, 6 males fed methyl cellulose, and 16 males and 10 females untreated. The guinea pigs were sacrificed at 42 days by suffocation in CO2. Liver samples were immediately immersed and minced in 2% gluteraldehyde in cacadylate buffer at pH 7.4 and 4°C. After overnight fixation, samples were postfixed in 1% OsO4 in cacodylate for 1 hr at room temperature, embedded in epon, sectioned and stained with uranyl acetate and lead citrate.

Sign in / Sign up

Export Citation Format

Share Document