Differential Expression of Cancer-Associated Proteins in Breastmilk

2013 ◽  
Vol 8 (1) ◽  
pp. 120-126 ◽  
Author(s):  
Wenyi Qin ◽  
Ke Zhang ◽  
Beth Kliethermes ◽  
Ramak Amjad ◽  
Kaitlin Clarke ◽  
...  
Keyword(s):  
Differential Expression ◽  
Associated Proteins

scholarly journals The Differential Expression of Myometrial Connexin-43, Cyclooxygenase-1 and -2, and Gsα Proteins in the Upper and Lower Segments of the Human Uterus during Pregnancy and Labor1

1999 ◽  
Vol 84 (5) ◽  
pp. 1705-1710 ◽  
Author(s):  
Colette Sparey ◽  
Stephen C. Robson ◽  
Jarrod Bailey ◽  
Fiona Lyall ◽  
G. Nicholas Europe-Finner
Keyword(s):  
Gap Junction ◽  
Differential Expression ◽  
Connexin 43 ◽  
Cervical Ripening ◽  
Human Uterus ◽  
Down Regulation ◽  
Specific Antibodies ◽  
Cyclooxygenase 1 ◽  
Associated Proteins ◽  
Cox 1

There is evidence from many studies indicating that a number of specific quiescent and contractile associated proteins are temporally regulated in the myometrium during pregnancy. In this present investigation we provide data that strongly suggest that myometrial connexin-43, cyclooxygenase-1 and -2 (COX-1 and -2), and Gsα proteins are also spatially expressed within the human uterus during pregnancy and labor. Using paired lower and upper segment myometrial samples taken from individual women at term and during spontaneous labor, we have measured the expression of these proteins by immunoblotting with specific antibodies. We report that the myometrial gap junction connexin-43 protein is expressed at much greater levels in the upper uterine compared to the lower uterine segment and that this difference is even more pronounced during the course of labor. Conversely, myometrial COX-1 and -2 proteins appear to be expressed at much greater levels in the lower compared to the upper uterine segment. Moreover, the level of expression of both proteins is unaffected by the onset of parturition. In contrast, myometrial Gsα protein appears to be uniformly expressed in both lower and upper segments and is similarly down-regulated during parturition, as previously reported. The differential expression of COX-1 and -2 and connexin-43 in the uterus may allow cervical ripening before and dilatation during labor and facilitate effective propagation of contractions from fundus to cervix, which may be further facilitated by the down-regulation of Gsα at the onset of parturition.

scholarly journals microRNA-199a-5p regulates epithelial-to-mesenchymal transition in diabetic cataract by targeting SP1 gene

2020 ◽  
Vol 26 (1) ◽  
Author(s):  
Xin Liu ◽  
Qiaoyun Gong ◽  
Longfei Yang ◽  
Min Liu ◽  
Lingzhi Niu ◽  
...  
Keyword(s):  
Differential Expression ◽  
Epithelial To Mesenchymal Transition ◽  
Specific Protein ◽  
Luciferase Reporter ◽  
Diabetic Cataract ◽  
Mesenchymal Transition ◽  
Associated Proteins ◽  
Precise Role ◽  
Dual Luciferase Reporter Assay

Abstract Background As a common ocular complication of diabetes mellitus, diabetic cataract is becoming a leading cause of visual impairment. The progression of diabetic cataract progression involves epithelial-to-mesenchymal transition (EMT), the precise role of which remains to be investigated. As microRNAs (miRNAs) are suggested to be involved in the pathogenesis of many diseases, identification of aberrantly expressed miRNAs in diabetic lens epithelial cells (LECs) and their targets may provide insights into our understanding of diabetic cataract and potential therapeutic targets. Methods Diabetic cataract capsules and LECs exposed to high glucose (25 mmol/L, 1–5 days) were used to mimic the model. Quantitative RT-PCR was performed to evaluate the differential expression of miRNA. Dual luciferase reporter assay was used to identify the binding target of miR-199a-5p. The expression of EMT-associated proteins was determined by immunofluorescence and Western blot analysis. Results Our results showed the differential expression of miR-9, -16, -22, -199a and -204. MiR-199a was downregulated in diabetic cataract capsule and hyperglycemia-conditioned human LECs. Specific protein 1 could be directly targeted and regulated by miR-199a in LECs and inhibit EMT in diabetic LECs. Conclusion Our findings implied miR-199a could be a therapeutic target by regulating SP1 directly to affect EMT in diabetic cataract and provided novel insights into the pathogenesis of diabetic cataract.

Actin Organization and Cell Migration of Melanoma Cells Relate to Differential Expression of Integrins and Actin-associated Proteins

1992 ◽  
Vol 19 (11) ◽  
pp. 847-852 ◽  
Author(s):  
Hugh Randolph Byers ◽  
Takafumi Etoh† ◽  
Jacqueline Vink ◽  
Nancy Franklin ◽  
Sebastiano Gattoni-Celli ◽  
...  
Keyword(s):  
Cell Migration ◽  
Differential Expression ◽  
Melanoma Cells ◽  
Actin Organization ◽  
Associated Proteins

scholarly journals Differential expression of dystrophin, utrophin and dystrophin-associated proteins in peripheral nerve

FEBS Letters ◽  
1993 ◽  
Vol 334 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Kiichiro Matsumura ◽  
Hiroki Yamada ◽  
Teruo Shimizu ◽  
Kevin P. Campbell
Keyword(s):  
Peripheral Nerve ◽  
Differential Expression ◽  
Associated Proteins

scholarly journals Effect of Rosiglitazone on the Differential Expression of Diabetes-associated Proteins in Pancreatic Islets of C57Bl/6lep/lepMice

2002 ◽  
Vol 1 (7) ◽  
pp. 509-516 ◽  
Author(s):  
Jean-Charles Sanchez ◽  
Véronique Converset ◽  
Anna Nolan ◽  
Gerhard Schmid ◽  
Steven Wang ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Differential Expression ◽  
Associated Proteins

scholarly journals Differential expression of cancer associated proteins in breast milk based on age at first full term pregnancy

BMC Cancer ◽  
2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Wenyi Qin ◽  
Ke Zhang ◽  
Beth Kliethermes ◽  
Rachel L Ruhlen ◽  
Eva P Browne ◽  
...  
Keyword(s):  
Breast Milk ◽  
Differential Expression ◽  
Full Term ◽  
Term Pregnancy ◽  
Associated Proteins
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