scholarly journals Studies on Culture and Osteogenic Induction of Human Mesenchymal Stem Cells under CO2-Independent Conditions

Astrobiology ◽  
2013 ◽  
Vol 13 (4) ◽  
pp. 370-379 ◽  
Author(s):  
Jian Chen ◽  
Cui Zhang ◽  
Yiding Feng ◽  
Chen Zong ◽  
Jiarong Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Osteogenic Induction

Osteogenic Induction of Human Mesenchymal Stem Cells by Cold Atmospheric Argon Plasma

2016 ◽  
Vol 6 (2) ◽  
pp. 193-207
Author(s):  
Adil Shahzad Ahmed ◽  
Joseph Chavarria ◽  
Tyler Brenneman ◽  
Kurt Johnson ◽  
Erik Antonsen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Argon Plasma ◽  
Osteogenic Induction ◽  
Atmospheric Argon

scholarly journals High Content Imaging of Early Morphological Signatures Predicts Long Term Mineralization Capacity of Human Mesenchymal Stem Cells upon Osteogenic Induction

Stem Cells ◽  
2016 ◽  
Vol 34 (4) ◽  
pp. 935-947 ◽  
Author(s):  
Ross A. Marklein ◽  
Jessica L. Lo Surdo ◽  
Ian H. Bellayr ◽  
Saniya A. Godil ◽  
Raj K. Puri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
High Content Imaging ◽  
Osteogenic Induction

scholarly journals Graphene Oxide Induced Osteogenesis Quantification by In-Situ 2D-Fluorescence Spectroscopy

2018 ◽  
Vol 19 (11) ◽  
pp. 3336 ◽  
Author(s):  
Valentina Palmieri ◽  
Marta Barba ◽  
Lorena Di Pietro ◽  
Claudio Conti ◽  
Marco De Spirito ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Human Mesenchymal Stem Cells ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
Tissue Sections ◽  
Osteogenic Induction ◽  
Calcium Deposits

Graphene and graphene oxide can promote the adhesion, growth and differentiation of mesenchymal stem cells. Further, graphene surface coatings accelerate the differentiation of human mesenchymal stem cells acting as osteogenic inducers. Quantification of the osteogenic induction is conventionally performed with Alizarin Red S (ARS), an anthraquinone derivative used to identify calcium deposits in tissue sections and cell cultures. The ARS staining is quite versatile because the dye forms an Alizarin Red S–calcium complex that can be extracted from the stained monolayer of cells and readily assayed by absorbance measurements. Direct visualization of stained deposits is also feasible; however, an in-situ visualization and quantification of deposits is possible only on transparent supports and not on thick opaque materials like ceramics and graphene composites that are well-known inducers of osteogenesis. In this manuscript, the shape of the 2D-fluorescence spectra of the ARS-calcium complex is used to develop a method to detect and monitor the in-situ differentiation process occurring during the osteogenic induction mediated by opaque graphene oxide surfaces.

scholarly journals Hypoxic-Mediated Oxidative Stress Condition and Hydroxyapatite-Inducing Osteogenic Differentiation of Human Mesenchymal Stem Cells: A Mathematical Modeling Study

2020 ◽  
Vol 16 (6) ◽  
pp. 910-921
Author(s):  
Yan Chao Cui ◽  
Yu Sheng Qiu ◽  
Qiong Wu ◽  
Gang Bu ◽  
Seoh Wei Teh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Bone Cell ◽  
Culture Conditions ◽  
Cell Replacement ◽  
Hypoxic Conditions ◽  
Replacement Treatment ◽  
Osteogenic Induction ◽  
Vessel Network

Avascular necrosis (AVN) of the bones remains a major clinical challenge. Fractures in the talus, the scaphoid, and the neck of the femur are especially challenging to heal due to the low blood vessel network and the lack of collateral blood supply. These fractures are associated with high rates of nonunion and increased infections that require repeated operations. Conventional treatments by autografting or allografting bone replacement and synthetic bone implants have limitations, including the invasiveness of operative procedures, tissue supply insufficiency, and the risk of host rejection. The advancement in tissue engineering has revealed the potential of stem cells as restorative agents for bone injuries. The administration of mesenchymal stem cells (MSCs) into the talus, the scaphoid, and the neck of the femur could produce enhanced osteogenesis via the manipulation of MSC culture conditions. In this study, we used hydroxyapatite as the nanomaterial, and hypoxic milieu to enhance MSC differentiation capacity into the osteogenic lineage, allowing for more rapid and efficient bone cell replacement treatment. Our results demonstrate 1% oxygen and 12.5 μg/mL of hydroxyapatite (HAP) as the optimal conditions to incorporate the osteogenic medium for the osteogenic induction of MSCs. We also established a proof of concept that the addition of HAP and hypoxic conditions could augment the osteoinductive capacity of MSCs. We also developed an accurate mathematical model to support future bone cell replacement therapy.

Tripeptide-based macroporous hydrogel improves the osteogenic microenvironment of stem cells

2021 ◽  
Author(s):  
Qian Li ◽  
He Zhang ◽  
Jijia Pan ◽  
Binhong Teng ◽  
Ziqian Zeng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
3D Culture ◽  
Human Mesenchymal Stem Cells ◽  
Osteogenic Induction

Due to the ability to combine multiple osteogenic induction "cues" at the same time, hydrogels are widely used in the three-dimensional (3D) culture of human mesenchymal stem cells (hMSCs) and...

scholarly journals Superior Osteo-Inductive and Osteo-Conductive Properties of Trabecular Titanium vs. PEEK Scaffolds on Human Mesenchymal Stem Cells: A Proof of Concept for the Use of Fusion Cages

2021 ◽  
Vol 22 (5) ◽  
pp. 2379
Author(s):  
Enrico Ragni ◽  
Carlotta Perucca Orfei ◽  
Alessandro Bidossi ◽  
Elena De Vecchi ◽  
Natale Francaviglia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Calcium Deposition ◽  
Lumbar Spine Surgery ◽  
Plastic Surface ◽  
Osteogenic Induction ◽  
Conductive Properties ◽  
Osteogenic Factors

Fusion cages composed of titanium and its alloys are emerging as valuable alternative to standard polyetheretherketone (PEEK) ones routinely used in cervical and lumbar spine surgery. Aim of this study was to evaluate osteo-inductive and osteo-conductive ability of an innovative trabecular titanium (T-Ti) scaffold on human mesenchymal stem cells (hMSCs), in both absence and presence of biochemical osteogenic stimuli. Same abilities were assessed on PEEK and standard 2D plastic surface, the latter meant as gold-standard for in vitro differentiation studies. hMSCs adhered and colonized both T-Ti and PEEK scaffolds. In absence of osteogenic factors, T-Ti triggered osteogenic induction of MSCs, as demonstrated by alkaline phosphatase activity and calcium deposition increments, while PEEK and standard 2D did not. Addition of osteogenic stimuli reinforced osteogenic differentiation of hMSCs cultured on T-Ti in a significantly higher manner with respect to standard 2D plastic culture surfaces, whereas PEEK almost completely abolished the process. T-Ti driven differentiation towards osteoblasts was confirmed by gene and marker expression analyses, even in absence of osteogenic stimuli. These results clearly indicate superior in vitro osteo-inductive and osteo-conductive capacity of T-Ti compared to PEEK, and make ground for further studies supporting the use of T-Ti cages to improve bone fusion.

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