scholarly journals Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins

2020 ◽  
Vol 32 (9) ◽  
pp. 636-655 ◽  
Author(s):  
Maya A. Olshina ◽  
Galina Arkind ◽  
Fanindra Kumar Deshmukh ◽  
Irit Fainer ◽  
Mark Taranavsky ◽  
...  
Keyword(s):  
20S Proteasome ◽  
Inhibitory Proteins

Theoretical Studies of the Acid-Base Equilibria in a Model Active Site of the Human 20S Proteasome

2020 ◽  
Author(s):  
Jon Uranga ◽  
Lukas Hasecke ◽  
Jonny Proppe ◽  
Jan Fingerhut ◽  
Ricardo A. Mata
Keyword(s):  
Active Site ◽  
Boronic Acid ◽  
Computational Study ◽  
Ubiquitin Proteasome System ◽  
Bayesian Optimization ◽  
Inhibition Mechanism ◽  
20S Proteasome ◽  
Acid Base ◽  
Ubiquitin Proteasome ◽  
Infection Cycles

The 20S Proteasome is a macromolecule responsible for the chemical step in the ubiquitin-proteasome system of degrading unnecessary and unused proteins of the cell. It plays a central role both in the rapid growth of cancer cells as well as in viral infection cycles. Herein, we present a computational study of the acid-base equilibria in an active site of the human proteasome, an aspect which is often neglected despite the crucial role protons play in the catalysis. As example substrates, we take the inhibition by epoxy and boronic acid containing warheads. We have combined cluster quantum mechanical calculations, replica exchange molecular dynamics and Bayesian optimization of non-bonded potential terms in the inhibitors. In relation to the latter, we propose an easily scalable approach to the reevaluation of non-bonded potentials making use of QM/MM dynamics information. Our results show that coupled acid-base equilibria need to be considered when modeling the inhibition mechanism. The coupling between a neighboring lysine and the reacting threonine is not affected by the presence of the inhibitor.

An antibody-based amperometric biosensor for 20S proteasome activity and inhibitor screening

The Analyst ◽  
2021 ◽  
Author(s):  
Madalina M. Barsan ◽  
Victor C. Diculescu
Keyword(s):  
Proteasome Activity ◽  
Electrochemical Methods ◽  
Amperometric Biosensor ◽  
20S Proteasome ◽  
Specific Interactions ◽  
Inhibitor Screening

The 20S proteasome is immobilized through specific interactions with antibodies and its activity is evaluated by electrochemical methods.

Proteomic Characterization of Human Erythrocyte 20S Proteasome and Analysis of Species-dependent 20S Proteasome Heterogeneity

2009 ◽  
Vol 37 (12) ◽  
pp. 1711-1716 ◽  
Author(s):  
Guo-Qiang CHEN ◽  
Hui LIU ◽  
Hai-Jing ZHANG ◽  
Yan-Chun DENG ◽  
Zhi-Li LI
Keyword(s):  
Human Erythrocyte ◽  
20S Proteasome

scholarly journals Regulation of Autophagic Flux by the 20S Proteasome

2019 ◽  
Vol 26 (9) ◽  
pp. 1283-1294.e5 ◽  
Author(s):  
Evert Njomen ◽  
Jetze J. Tepe
Keyword(s):  
Autophagic Flux ◽  
20S Proteasome
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