Reactive Oxygen Species and Their Implications on CD4+T Cells in Type 1 Diabetes

2018 ◽  
Vol 29 (14) ◽  
pp. 1399-1414 ◽  
Author(s):  
Dana M. Previte ◽  
Jon D. Piganelli
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Type 1 Diabetes ◽  
Cd4 T Cells ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals NADPH Oxidase-Derived Overproduction of Reactive Oxygen Species Impairs Postischemic Neovascularization in Mice with Type 1 Diabetes

2006 ◽  
Vol 169 (2) ◽  
pp. 719-728 ◽  
Author(s):  
Téni G Ebrahimian ◽  
Christophe Heymes ◽  
Dong You ◽  
Olivier Blanc-Brude ◽  
Barend Mees ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Type 1 Diabetes ◽  
Nadph Oxidase ◽  
Reactive Oxygen ◽  
Oxygen Species

Reactive oxygen species induce signals that lead to apoptotic DNA degradation in primary CD4+ T cells

APOPTOSIS ◽  
2005 ◽  
Vol 10 (6) ◽  
pp. 1433-1443 ◽  
Author(s):  
M. Pajusto ◽  
T. H. Toivonen ◽  
J. Tarkkanen ◽  
E. Jokitalo ◽  
P. S. Mattila
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Cd4 T Cells ◽  
Reactive Oxygen ◽  
Dna Degradation ◽  
Oxygen Species

Reactive Oxygen Species Damaged Hemoglobin Presents Unique Epitopes for Type 1 Diabetes Autoantibodies

2007 ◽  
Vol 2 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Zafar Rasheed ◽  
Rizwan Ahmad ◽  
Naila Rasheed ◽  
Trivendra Tripathi ◽  
Rashid Ali
Keyword(s):  
Reactive Oxygen Species ◽  
Type 1 Diabetes ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Diabetes Autoantibodies

scholarly journals Enhanced Reactive Oxygen Species Production, Acidic Cytosolic pH and Upregulated Na+/H+ Exchanger (NHE) in Dicer Deficient CD4+ T Cells

2017 ◽  
Vol 42 (4) ◽  
pp. 1377-1389 ◽  
Author(s):  
Yogesh Singh ◽  
Yuetao Zhou ◽  
Shaqiu Zhang ◽  
Khalid N.M. Abdelazeem ◽  
Bernat Elvira ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
T Cells ◽  
Cell Volume ◽  
Cd4 T Cells ◽  
Reactive Oxygen ◽  
Transcriptional Level ◽  
Ros Production ◽  
Oxygen Species ◽  
Protein Levels ◽  
And Function

Background: MicroRNAs (miRNAs) negatively regulate gene expression at a post-transcriptional level. Dicer, a cytoplasmic RNase III enzyme, is required for the maturation of miRNAs from precursor miRNAs. Dicer, therefore, is a critical enzyme involved in the biogenesis and processing of miRNAs. Several biological processes are controlled by miRNAs, including the regulation of T cell development and function. T cells generate reactive oxygen species (ROS) with parallel H+ extrusion accomplished by the Na+/H+-exchanger 1 (NHE1). The present study explored whether ROS production, as well as NHE1 expression and function are sensitive to the lack of Dicer (miRNAs deficient) and could be modified by individual miRNAs. Methods: CD4+ T cells were isolated from CD4 specific Dicer deficient (DicerΔ/Δ) mice and the respective control mice (Dicerfl/fl). Transcript and protein levels were quantified with RT-PCR and Western blotting, respectively. For determination of intracellular pH (pHi) cells were incubated with the pH sensitive dye bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) and Na+/H+ exchanger (NHE) activity was calculated from re-alkalinization after an ammonium pulse. Changes in cell volume were measured using the forward scatter in flow cytometry, and ROS production utilizing 2',7' -dichlorofluorescin diacetate (DCFDA) fluorescence. Transfection of miRNA-control and mimics in T cells was performed using DharmaFECT3 reagent. Results: ROS production, cytosolic H+ concentration, NHE1 transcript and protein levels, NHE activity, and cell volume were all significantly higher in CD4+ T cells from DicerΔ/Δ mice than in CD4+ T cells from Dicerfl/fl mice. Furthermore, individual miR-200b and miR-15b modify pHi and NHE activity in Dicerfl/fl and DicerΔ/Δ CD4+ T cells, respectively. Conclusions: Lack of Dicer leads to oxidative stress, cytosolic acidification, upregulated NHE1 expression and activity as well as swelling of CD4+ T cells, functions all reversed by miR-15b or miR-200b.

scholarly journals Mitochondrial Reactive Oxygen Species and Type 1 Diabetes

2018 ◽  
Vol 29 (14) ◽  
pp. 1361-1372 ◽  
Author(s):  
Jing Chen ◽  
Scott E. Stimpson ◽  
Gabriel A. Fernandez-Bueno ◽  
Clayton E. Mathews
Keyword(s):  
Reactive Oxygen Species ◽  
Type 1 Diabetes ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mitochondrial Reactive Oxygen Species

scholarly journals The role of reactive oxygen species and proinflammatory cytokines in type 1 diabetes pathogenesis

2013 ◽  
Vol 1281 (1) ◽  
pp. 16-35 ◽  
Author(s):  
Lindsey E. Padgett ◽  
Katarzyna A. Broniowska ◽  
Polly A. Hansen ◽  
John A. Corbett ◽  
Hubert M. Tse
Keyword(s):  
Reactive Oxygen Species ◽  
Type 1 Diabetes ◽  
Proinflammatory Cytokines ◽  
Reactive Oxygen ◽  
Oxygen Species
Sign in / Sign up

Export Citation Format

Share Document