Conditionally Pathogenic Gut Microbes Promote Larval Growth by Increasing Redox-Dependent Fat Storage in High-Sugar Diet-Fed Drosophila

2017 ◽  
Vol 27 (16) ◽  
pp. 1361-1380 ◽  
Author(s):  
Tae Woong Whon ◽  
Na-Ri Shin ◽  
Mi-Ja Jung ◽  
Dong-Wook Hyun ◽  
Hyun Sik Kim ◽  
...  
Keyword(s):  
Larval Growth ◽  
Fat Storage ◽  
Gut Microbes ◽  
High Sugar

scholarly journals Gene–Diet Interactions: Dietary Rescue of Metabolic Defects in spen-Depleted Drosophila melanogaster

Genetics ◽  
2020 ◽  
Vol 214 (4) ◽  
pp. 961-975 ◽  
Author(s):  
Claire M. Gillette ◽  
Kelsey E. Hazegh ◽  
Travis Nemkov ◽  
Davide Stefanoni ◽  
Angelo D’Alessandro ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Complex Disease ◽  
Dominant Factor ◽  
Glycolytic Flux ◽  
Metabolic Dysfunction ◽  
Fat Storage ◽  
Developmental Defects ◽  
Larval Stages ◽  
High Sugar ◽  
Metabolic Defects

Obesity and its comorbidities are a growing health epidemic. Interactions between genetic background, the environment, and behavior (i.e., diet) greatly influence organismal energy balance. Previously, we described obesogenic mutations in the gene Split ends (Spen) in Drosophila melanogaster, and roles for Spen in fat storage and metabolic state. Lipid catabolism is impaired in Spen-deficient fat storage cells, accompanied by a compensatory increase in glycolytic flux and protein catabolism. Here, we investigate gene–diet interactions to determine if diets supplemented with specific macronutrients can rescue metabolic dysfunction in Spen-depleted animals. We show that a high-yeast diet partially rescues adiposity and developmental defects. High sugar partially improves developmental timing as well as longevity of mated females. Gene–diet interactions were heavily influenced by developmental-stage-specific organismal needs: extra yeast provides benefits early in development (larval stages) but becomes detrimental in adulthood. High sugar confers benefits to Spen-depleted animals at both larval and adult stages, with the caveat of increased adiposity. A high-fat diet is detrimental according to all tested criteria, regardless of genotype. Whereas Spen depletion influenced phenotypic responses to supplemented diets, diet was the dominant factor in directing the whole-organism steady-state metabolome. Obesity is a complex disease of genetic, environmental, and behavioral inputs. Our results show that diet customization can ameliorate metabolic dysfunction underpinned by a genetic factor.

scholarly journals Disrupting butterfly caterpillar microbiomes does not impact their survival and development

2019 ◽  
Vol 286 (1917) ◽  
pp. 20192438 ◽  
Author(s):  
Kruttika Phalnikar ◽  
Krushnamegh Kunte ◽  
Deepa Agashe
Keyword(s):  
Larval Growth ◽  
Biomass Accumulation ◽  
Larval Survival ◽  
Gut Bacteria ◽  
Growth And Survival ◽  
Gut Microbes ◽  
Survival And Development ◽  
Similar Survival ◽  
And Control ◽  
Butterfly Larvae

Associations with gut microbes are believed to play crucial roles in the physiology, immune function, development and behaviour of insects. However, microbiome sequencing has recently suggested that butterflies are an anomaly, because their microbiomes do not show strong host- and developmental stage-specific associations. We experimentally manipulated butterfly larval gut microbiota and found that disrupting gut microbes had little influence on larval survival and development. Larvae of the butterflies Danaus chrysippus and Ariadne merione that fed on chemically sterilized or antibiotic-treated host plant leaves had significantly reduced bacterial loads, and their gut bacterial communities were disrupted substantially. However, neither host species treated this way suffered a significant fitness cost: across multiple experimental blocks, treated and control larvae had similar survival, growth and development. Furthermore, re-introducing microbes from the excreta of control larvae did not improve larval growth and survival. Thus, these butterfly larvae did not appear to rely on specialized gut bacteria for digestion, detoxification, biomass accumulation and metamorphosis. Our experiments thus show that dependence on gut bacteria for growth and survival is not a universal phenomenon across insects. Our findings also caution that strategies which target gut microbiomes may not always succeed in controlling Lepidopteran pests.

Larval Behavior and Protective Implications for Sea Scallops and Surf Clams within the Middle Atlantic Bight: Part Two, Variability of Physical Environment and Larval Behaviors

2019 ◽  
Vol 2 (2) ◽  
Keyword(s):  
Physical Environment ◽  
Climate Changes ◽  
Larval Growth ◽  
Growth Patterns ◽  
Larval Behavior ◽  
Larval Release ◽  
Physical Conditions ◽  
Climatic Variations ◽  
Middle Atlantic ◽  
Sea Scallops

Larval growth and settlement rates are important larval behaviors for larval protections. The variability of larval growthsettlement rates and physical conditions for 2006-2012 and in the future with potential climate changes was studied using the coupling ROMS-IMBs, and new temperature and current indexes. Forty-four experimental cases were conducted for larval growth patterns and release mechanisms, showing the spatial, seasonal, annual, and climatic variations of larval growthsettlement rates and physical conditions, demonstrating that the slight different larval temperature-adaption and larval release strategies made difference in larval growth-settlement rates, and displaying that larval growth and settlement rates highly depended upon physical conditions and were vulnerable to climate changes.

Spatial ecology and growth in early life stages of bay anchovy Anchoa mitchilli in Chesapeake Bay (USA)

2020 ◽  
Vol 651 ◽  
pp. 125-143
Author(s):  
TD Auth ◽  
T Arula ◽  
ED Houde ◽  
RJ Woodland
Keyword(s):  
Chesapeake Bay ◽  
Spatial Ecology ◽  
Environmental Variability ◽  
Larval Growth ◽  
Larval Abundance ◽  
Spatial And Temporal Variability ◽  
Estuarine Ecosystem ◽  
Anchoa Mitchilli ◽  
Larval Production ◽  
Bay Anchovy

The bay anchovy Anchoa mitchilli is the most abundant fish in Chesapeake Bay (USA) and is a vital link between plankton and piscivores within the trophic structure of this large estuarine ecosystem. Baywide distributions and abundances of bay anchovy eggs and larvae, and larval growth, were analyzed in a 5 yr program to evaluate temporal and spatial variability based on research surveys in the 1995-1999 spawning seasons. Effects of environmental variability and abundance of zooplankton that serve as prey for larval bay anchovy were analyzed. In the years of these surveys, 97.6% of eggs and 98.8% of larvae occurred in the polyhaline lower bay. Median egg and larval abundances differed more than 10-fold for surveys conducted in the 5 yr and were highest in the lower bay. Within years, median larval abundance (ind. m-2) in the lower bay was generally 1-2 orders of magnitude higher than upper-bay abundance. Salinity, temperature, and dissolved oxygen explained 12% of the spatial and temporal variability in egg abundances and accounted for 27% of the variability in larval abundances. The mean, baywide growth rate for larvae over the 5 yr period was 0.75 ± 0.01 mm d-1, and was best explained by zooplankton concentration and feeding incidence. Among years, mean growth rates ranged from 0.68 (in 1999) to 0.81 (in 1998) mm d-1 and were fastest in the upper bay. We identified environmental factors, especially salinity, that contributed to broadscale variability in egg and larval production.

The box-balance model: a new tool to assess fish larval survival, applied to field data on two small pelagic fish

2020 ◽  
Vol 650 ◽  
pp. 289-308 ◽  
Author(s):  
V Raya ◽  
J Salat ◽  
A Sabatés
Keyword(s):  
Field Data ◽  
Fish Larvae ◽  
Larval Growth ◽  
Warming Trend ◽  
Mortality Rates ◽  
Larval Survival ◽  
Pelagic Fish ◽  
Balance Model ◽  
Small Pelagic Fish ◽  
Mesoscale Structures

This work develops a new method, the box-balance model (BBM), to assess the role of hydrodynamic structures in the survival of fish larvae. The BBM was applied in the northwest Mediterranean to field data, on 2 small pelagic fish species whose larvae coexist in summer: Engraulis encrasicolus, a dominant species, and Sardinella aurita, which is expanding northwards in relation to sea warming. The BBM allows one to quantify the contribution of circulation, with significant mesoscale activity, to the survival of fish larvae, clearly separating the effect of transport from biological factors. It is based on comparing the larval abundances at age found in local target areas, associated with the mesoscale structures (boxes), to those predicted by the overall mortality rate of the population in the region. The application of the BBM reveals that dispersion/retention by hydrodynamic structures favours the survival of E. encrasicolus larvae. In addition, since larval growth and mortality rates of the species are required parameters for application of the BBM, we present their estimates for S. aurita in the region for the first time. Although growth and mortality rates found for S. aurita are both higher than for E. encrasicolus, their combined effect confers a lower survival to S. aurita larvae. Thus, although the warming trend in the region would contribute to the expansion of the fast-growing species S. aurita, we can confirm that E. encrasicolus is well established, with a better adapted survival strategy.

Identification of Cross Reactive Insulin Immunogenic Epitopes from Commensal Gut Microbes

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 95-OR ◽  
Author(s):  
EMRAH ALTINDIS ◽  
ANTHONY N. VOMUND ◽  
I-TING CHOW ◽  
MARCOS DAMASIO ◽  
WILLIAM KWOK ◽  
...  
Keyword(s):  
Gut Microbes
Sign in / Sign up

Export Citation Format

Share Document