scholarly journals Preclinical Evaluation of Targeting the Nrf2 Pathway by Triterpenoids (CDDO-Im and CDDO-Me) for Protection from LPS-Induced Inflammatory Response and Reactive Oxygen Species in Human Peripheral Blood Mononuclear Cells and Neutrophils

2007 ◽  
Vol 9 (11) ◽  
pp. 1963-1970 ◽  
Author(s):  
Rajesh K. Thimmulappa ◽  
Ralph J. Fuchs ◽  
Deepti Malhotra ◽  
Catherine Scollick ◽  
Kassim Traore ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Inflammatory Response ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Oxygen Species ◽  
Peripheral Blood Mononuclear ◽  
Nrf2 Pathway ◽  
Preclinical Evaluation ◽  
Blood Mononuclear Cells

scholarly journals cAMP Activates The generation of Reactive Oxygen Species and Inhibits The Secretion of IL-6 in Peripheral Blood Mononuclear Cells from Type 2 Diabetic Patients

2009 ◽  
Vol 2 (5) ◽  
pp. 317-321 ◽  
Author(s):  
Camila Armond Isoni ◽  
Érica Abreu Borges ◽  
Clara Araújo Veloso ◽  
Rafael Teixeira Mattos ◽  
Miriam Martins Chaves ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
In Cells

Peripheral blood mononuclear cells (PBMNC) from patients with type 2 diabetes (DM2) have generated higher levels of reactive oxygen species (ROS) that were higher than those in cells from healthy individuals. In the presence of a cAMP-elevating agent, ROS production was significantly activated in PBMNC from DM2 patients but it was inhibited in cells from healthy subjects. Higher levels of IL-6 has been detected in the supernatant of PBMNC cultures from DM2 patients in comparison with healthy controls. When cells were cultured in the presence of a cAMP-elevating agent, the level of IL-6 decreased has by 46% in the supernatant of PBMNC from DM2 patients but it remained unaltered in controls. No correlations between ROS and IL-6 levels in PBMNC from DM2 patients or controls have been observed. Secretions of IL-4 or IFN by PBMNC from patients or controls have not been affected by the elevation of cAMP. cAMP elevating agents have activated the production of harmful reactive oxidant down modulated IL-6 secretion by these cells from DM2 patients, suggesting an alteration in the metabolic response possibly due to hyperglicemia. The results suggest that cAMP may play an important role in the pathogenesis of diabetes.

Variability of sinapic acid derivatives during germination and their contribution to antioxidant and anti-inflammatory effects of broccoli sprouts on human plasma and human peripheral blood mononuclear cells

2020 ◽  
Vol 11 (8) ◽  
pp. 7231-7244 ◽  
Author(s):  
Monika Anna Olszewska ◽  
Sebastian Granica ◽  
Joanna Kolodziejczyk-Czepas ◽  
Anna Magiera ◽  
Monika Ewa Czerwińska ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Human Plasma ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Sinapic Acid ◽  
Peripheral Blood Mononuclear ◽  
Broccoli Sprouts ◽  
Blood Mononuclear Cells ◽  
Broccoli Sprout

Broccoli sprout extract rich in sinapic acid derivatives prevents oxidative changes in human plasma and modulates inflammatory response in PBMCs.

scholarly journals Semi-Refined Carrageenan Attenuates Lipopolysaccharide-Induced Reactive Oxygen Species Production in Peripheral Blood Mononuclear Cells and Cell Membrane Alterations in Leukocytes

2021 ◽  
Vol 11 ◽  
pp. 175-183
Author(s):  
Yevgen Posokhov ◽  
Anatolii Onishchenko ◽  
Tetyana Chumachenko ◽  
Nataliia Makieieva ◽  
Yuliia Kalashnyk-Vakulenko ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Membrane ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
Membrane Lipid ◽  
Ros Generation ◽  
Oxygen Species ◽  
Peripheral Blood Mononuclear ◽  
Lipid Order ◽  
Blood Mononuclear Cells

Aim: To assess the effects of semi-refined carrageenan (E407a) on lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) generation in peripheral blood mononuclear cells (PBMCs) and LPS-mediated cell membrane alterations in leukocytes. Methods: Blood samples collected from 8 intact rats were incubated with E407a (10 mg/ml), E407a (50 mg/ml), LPS (1 µg/ml), E407a (10 mg/ml) + LPS (1 µg/ml), E407a (50 mg/ml) + LPS (1 µg/ml) and without those compounds (controls) for 2 h in RPMI 1640 medium enriched with 5% fetal bovine serum. ROS generation in PBMCs obtained from the incubated samples was estimated by flow cytometry using 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining. The impact of E407a, LPS and their mixture on leukocyte cell membranes was evaluated spectrofluorimetrically using the fluorescent probe 2-(2¢-hydroxy-phenyl)-5-phenyl-1,3-oxazole. Results: Expectedly, incubation with LPS induced ROS generation in PBMCs and decreased the lipid order of cell membranes in leukocytes. E407a alone was found to alter neither ROS production in PBMCs, nor membrane lipid order in leukocytes. Semi-refined carrageenan partially reduced LPS-mediated ROS overproduction in PBMCs and cell membrane alterations in leukocytes. Conclusion: E407a attenuates LPS-induced alterations of redox homeostasis in rat PBMCs and LPS-mediated modifications of cell membrane lipid order in leukocytes.

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