HIV-1revAntisense Phosphorothioate Oligonucleotide Binding to Human Mononuclear Cells Is Cell Type Specific and Inducible

1994 ◽  
Vol 4 (4) ◽  
pp. 285-289 ◽  
Author(s):  
SAMUEL J. PIRRUCCELLO ◽  
GREG A. PERRY ◽  
PAUL J. BOCK ◽  
MOLLY S. LANG ◽  
SCOTT M. NOEL ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Cell Type ◽  
Phosphorothioate Oligonucleotide ◽  
Human Mononuclear Cells ◽  
Cell Type Specific ◽  
Oligonucleotide Binding

Cell-type-specific downregulation of heme oxygenase-1 by lipopolysaccharide via Bach1 in primary human mononuclear cells

2015 ◽  
Vol 78 ◽  
pp. 224-232 ◽  
Author(s):  
Mirrin J. Dorresteijn ◽  
Ananta Paine ◽  
Eva Zilian ◽  
Maaike G.E. Fenten ◽  
Eileen Frenzel ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Mononuclear Cells ◽  
Heme Oxygenase 1 ◽  
Cell Type ◽  
Human Mononuclear Cells ◽  
Cell Type Specific

A single-cell map for the transcriptomic signatures of peripheral blood mononuclear cells in end-stage renal disease

2019 ◽  
Author(s):  
Ting Luo ◽  
Fengping Zheng ◽  
Kang Wang ◽  
Yong Xu ◽  
Huixuan Xu ◽  
...  
Keyword(s):  
Single Cell ◽  
End Stage Renal Disease ◽  
Mononuclear Cells ◽  
Cell Types ◽  
Cell Type ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Cell Type Specific ◽  
Stage Renal Disease ◽  
End Stage

Abstract Background Immune aberrations in end-stage renal disease (ESRD) are characterized by systemic inflammation and immune deficiency. The mechanistic understanding of this phenomenon remains limited. Methods We generated 12 981 and 9578 single-cell transcriptomes of peripheral blood mononuclear cells (PBMCs) that were pooled from 10 healthy volunteers and 10 patients with ESRD by single-cell RNA sequencing. Unsupervised clustering and annotation analyses were performed to cluster and identify cell types. The analysis of hallmark pathway and regulon activity was performed in the main cell types. Results We identified 14 leukocytic clusters that corresponded to six known PBMC types. The comparison of cells from ESRD patients and healthy individuals revealed multiple changes in biological processes. We noticed an ESRD-related increase in inflammation response, complement cascade and cellular metabolism, as well as a strong decrease in activity related to cell cycle progression in relevant cell types in ESRD. Furthermore, a list of cell type-specific candidate transcription factors (TFs) driving the ESRD-associated transcriptome changes was identified. Conclusions We generated a distinctive, high-resolution map of ESRD-derived PBMCs. These results revealed cell type-specific ESRD-associated pathways and TFs. Notably, the pooled sample analysis limits the generalization of our results. The generation of larger single-cell datasets will complement the current map and drive advances in therapies that manipulate immune cell function in ESRD.

scholarly journals Mitochondrial profiling reveals dynamic, sex- and age-specific mitochondrial phenotypes in human immune cell subtypes

2020 ◽  
Author(s):  
Shannon Rausser ◽  
Caroline Trumpff ◽  
Marlon A McGill ◽  
Alex Junker ◽  
Wei Wang ◽  
...  
Keyword(s):  
Respiratory Chain ◽  
Repeated Measures ◽  
Mononuclear Cells ◽  
Immune Cell ◽  
Cell Type ◽  
Mitochondrial Content ◽  
Peripheral Blood Mononuclear ◽  
Mitochondrial Dna Copy Number ◽  
Age Related ◽  
Cell Type Specific

AbstractHow mitochondria functionally differ between immune cell subtypes, between the sexes, and with aging has not been defined in humans. We deploy a high-throughput mitochondrial phenotyping platform to define cell-type specific mitochondrial features in human circulating immune cell subtypes. In women and men spanning four decades of life, we find that mitochondrial content, mitochondrial DNA copy number (mtDNAcn), and respiratory chain enzymatic activities vary by up to 3.5-fold between neutrophils, monocytes, B and T lymphocyte subtypes. Mitochondria exhibit specific age- and sex-related differences among individual cell subtypes, including an age-related increase in mtDNAcn. In an intensive repeated-measures study, we also identify remarkable weekly variation in mitochondrial content and respiratory chain function, which is partially correlated with changes in circulating biomarkers. Our results also define multivariate mitochondrial phenotypes – mitotypes – that distinguish lymphoid from myeloid cell types, naïve-to-memory lymphocyte states, and moderately differ between women and men. Finally, a comparison of mitochondrial features in purified cell subtypes and in peripheral blood mononuclear cells (PBMCs) invites caution in using cell type mixtures to infer person-level mitochondrial function. Together, these findings identify dynamic cell-type specific variation in mitochondrial biology among circulating leukocytes and provide foundational knowledge to develop interpretable blood-based assays of mitochondrial health.

scholarly journals Single-cell RNA sequencing reveals cell-type specific cis-eQTLs in peripheral blood mononuclear cells

2017 ◽  
Author(s):  
M.G.P. van der Wijst ◽  
H. Brugge ◽  
D.H. de Vries ◽  
L.H. Franke
Keyword(s):  
Risk Factors ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Genetic Risk ◽  
Mononuclear Cells ◽  
Rna Seq ◽  
Cell Type ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Cell Type Specific

AbstractMost disease-associated genetic risk factors are regulatory. Here, we generated single-cell RNA-seq data of ∼25,000 peripheral blood mononuclear cells from 45 donors to identify how genetic variants affect gene expression. We validated this approach by replicating previously published whole blood RNA-seq cis-expression quantitative trait loci effects (cis-eQTLs), but also identified new cell type-specific cis-eQTLs. These eQTLs give additional insight into the downstream consequences of genetic risk factors for immune-mediated diseases.

Soluble VCAM-1 binding to α4 integrins is cell-type specific and activation dependent and is disrupted during apoptosis in T cells

Blood ◽  
2000 ◽  
Vol 95 (2) ◽  
pp. 602-609 ◽  
Author(s):  
David M. Rose ◽  
Pina M. Cardarelli ◽  
Ronald R. Cobb ◽  
Mark H. Ginsberg
Keyword(s):  
Mononuclear Cells ◽  
Atp Synthesis ◽  
Early Event ◽  
Cell Type ◽  
Cellular Functions ◽  
Peripheral Blood Mononuclear ◽  
Cellular Processes ◽  
High Affinity ◽  
Cell Type Specific ◽  
Induced Apoptosis

Soluble vascular cell adhesion molecule-1 (sVCAM-1) is generated during inflammation and can alter lymphocyte functions. The authors report that the binding of sVCAM-1 to 4 integrin-bearing cells is a dynamically regulated, active cellular process. Binding of recombinant sVCAM-1 to 4 integrins on peripheral blood mononuclear cells was cell-type specific. Circulating CD16+ NK cells constitutively bound sVCAM-1 with high affinity, whereas a subpopulation of T-lymphocytes, primarily CD45RO+ (memory), bound sVCAM-1 only after phorbol ester stimulation. sVCAM-1 binding to homogenous stable cell lines was also cell-type specific, and required active cellular processes because it was blocked by the inhibition of ATP synthesis and by Fas-induced apoptosis. Indeed, the loss of high-affinity VCAM-1 binding was an early event in apoptosis. Furthermore, an H-Ras/Raf-initiated signaling pathway also suppressed sVCAM-1 binding to 4β1 integrins. Collectively, these results showed that the capacity of 4 integrins to bind VCAM-1 is actively regulated and that this regulation may control 4 integrin-dependent cellular functions.

IFIH1-deficiency results in a cell-type specific alteration of autophagic activity in response to S. typhimurium

2017 ◽  
Vol 55 (05) ◽  
pp. e28-e56
Author(s):  
S Macheiner ◽  
R Gerner ◽  
A Pfister ◽  
A Moschen ◽  
H Tilg
Keyword(s):  
Cell Type ◽  
A Cell ◽  
Cell Type Specific ◽  
Autophagic Activity ◽  
Specific Alteration

The effect of 1,25(OH)2D3 on interferon i secretion by human mononuclear cells in vitro

2017 ◽  
Author(s):  
Lambros Athanassiou ◽  
Andrianos Nezos ◽  
Ifigenia Kostoglou-Athanassiou ◽  
Clio Mavragani ◽  
Panagiotis Athanassiou ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Human Mononuclear Cells
Sign in / Sign up

Export Citation Format

Share Document