Near Full-Length Genome Sequence of a Novel HIV-1 Second-Generation Recombinant Form (CRF01_AE/CRF07_BC) Detected Among Men Who Have Sex with Men in Tianjin, China

2019 ◽  
Vol 35 (2) ◽  
pp. 205-212 ◽  
Author(s):  
Ping Ma ◽  
Zhangwen Ge ◽  
Yi Feng ◽  
Weidong Ou ◽  
Jia Sun ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Second Generation ◽  
Full Length ◽  
Recombinant Form ◽  
Sex With Men ◽  
Full Length Genome ◽  
Hiv 1

scholarly journals Identification of CRF89_BF, a new member of an HIV-1 circulating BF intersubtype recombinant form family widely spread in South America

2021 ◽  
Author(s):  
Elena Delgado ◽  
Aurora Fernández-García ◽  
Ismael Fernández-Miranda ◽  
Sonia Benito ◽  
Vanesa Montero ◽  
...  
Keyword(s):  
South America ◽  
Genome Sequence ◽  
Full Length ◽  
Substantial Proportion ◽  
Recombinant Form ◽  
South American ◽  
Sequence Analyses ◽  
Full Length Genome ◽  
South American Origin ◽  
Hiv 1

Abstract Circulating recombinant forms (CRFs) represent a substantial proportion of infections in the HIV-1 pandemic. Among 103 CRFs described in the literature, 16 are BF intersubtype recombinants, most of South American origin, of which CRF12_BF is the most widely spread. A BF recombinant cluster identified in Bolivia was suggested to represent a new CRF_BF. Here, we find that this cluster belongs to a larger cluster incorporating 38 viruses collected in 7 countries from 3 continents, 21 of them in Spain, most from Bolivian or Peruvian individuals, and 12 in South America (Bolivia, Argentina, and Peru). It comprises three major subclusters, two associated with Bolivian individuals and one associated with Peruvian individuals. Near full-length genome sequence analyses of five viruses collected in Spain, Bolivia, and Peru revealed coincident BF mosaic structures, with 13 breakpoints, 5 and 6 of which coincided with CRF12_BF and CRF17_BF, respectively, and grouping in a clade closely related to these CRFs and more distantly to CRF38_BF and CRF44_BF, all circulating in South America. These results allow us to identify a new HIV-1 CRF, designated CRF89_BF. Through phylodynamic analyses, CRF89_BF emergence was estimated in Bolivia around 1984. CRF89_BF is the fifth CRF member of the HIV-1 recombinant family related to CRF12_BF

scholarly journals Identification of CRF89_BF, a new member of an HIV-1 circulating BF intersubtype recombinant form family widely spread in South America

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Elena Delgado ◽  
Aurora Fernández-García ◽  
Marcos Pérez-Losada ◽  
María Moreno-Lorenzo ◽  
Ismael Fernández-Miranda ◽  
...  
Keyword(s):  
South America ◽  
Phylogenetic Tree ◽  
Genome Sequence ◽  
Full Length ◽  
Recombinant Form ◽  
South American ◽  
Sequence Analyses ◽  
Full Length Genome ◽  
South American Origin ◽  
Hiv 1

AbstractCirculating recombinant forms (CRFs) contribute substantially to the HIV-1 pandemic. Among 105 CRFs described in the literature, 16 are BF intersubtype recombinants, most of South American origin, of which CRF12_BF is the most widely spread. A BF recombinant cluster identified in Bolivia was suggested to represent a new CRF_BF. Here we find that it belongs to a larger cluster incorporating 39 viruses collected in 7 countries from 3 continents, 22 of them in Spain, most from Bolivian or Peruvian individuals, and 12 in South America (Bolivia, Argentina, and Peru). This BF cluster comprises three major subclusters, two associated with Bolivian and one with Peruvian individuals. Near full-length genome sequence analyses of nine viruses, collected in Spain, Bolivia, and Peru, revealed coincident BF mosaic structures, with 13 breakpoints, 6 and 7 of which coincided with CRF12_BF and CRF17_BF, respectively. In a phylogenetic tree, they grouped in a clade closely related to these CRFs, and more distantly to CRF38_BF and CRF44_BF, all circulating in South America. These results allowed to identify a new HIV-1 CRF, designated CRF89_BF. Through phylodynamic analyses, CRF89_BF emergence was estimated in Bolivia around 1986. CRF89_BF is the fifth CRF member of the HIV-1 recombinant family related to CRF12_BF.

The identification of a novel HIV-1 second-generation recombinant form (CRF01_AE/CRF07_BC) among men who have sex with men in Jiangsu, China

2020 ◽  
Vol 18 ◽  
Author(s):  
Yin Yueqi ◽  
Zhou Ying ◽  
Lu Jing ◽  
Guo Hongxiong ◽  
Chen Jianshuang ◽  
...  
Keyword(s):  
Maximum Likelihood ◽  
Phylogenetic Trees ◽  
Second Generation ◽  
Emergency Situation ◽  
The World ◽  
Break Points ◽  
Sex With Men ◽  
Full Length Genome ◽  
Virus Strains ◽  
Hiv 1

Background: CRF01_AE and CRF07_BC are the two major HIV-1 virus strains circulating in China. The proportion of dominant subtypes (CRF01_AE and CRF07_BC) among MSM in Jiangsu province was over 80%. A large number of URFs have been found in China in recently years. Objective: This study aimed to report on novel HIV-1 recombinants. Method: We constructed Phylogenetic trees using the maximum likelihood (ML) method with 1000 bootstrap replicates in IQ-TREE 1.6.8 software and determined recombination break points using SimPlot 3.5.1. Results: We identified a novel, second-generation HIV-1 recombinant (JS020202) between CRF01_AE and CRF07_BC. The analysis of near full-length genome (NFLG) showed there were at least 8 breakpoints inner virus, which differed from any previously identified CRF and URF around the world. Conclusion: Novel diverse CRF01_AE/07_BC suggested the complexity trends of HIV-1 genetics. The emergency situation of diverse recombinant strains should be monitored continuously.

scholarly journals Near Full-Length Genome Identification of a Novel HIV-1 Recombinant Form (CRF01_AE/B′/C) Among Heterosexuals in Jilin, China

2014 ◽  
Vol 30 (7) ◽  
pp. 695-700 ◽  
Author(s):  
Xingguang Li ◽  
Chuanyi Ning ◽  
Yanli Chen ◽  
Yi Feng ◽  
Min Wei ◽  
...  
Keyword(s):  
Full Length ◽  
Recombinant Form ◽  
Full Length Genome ◽  
Hiv 1

scholarly journals Genetic Characterization and Recombinant History of a Novel Emerging HIV-1 Second-Generation Circulating Recombinant Form (CRF120_0107) Identified Among Sexually Transmitted Infections in Shenzhen, China

2021 ◽  
Author(s):  
Bo Zhu ◽  
Jin Zhao ◽  
Xiaorui Wang ◽  
Hanping Li ◽  
Yongjian Liu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Second Generation ◽  
Dual Infection ◽  
Sexually Transmitted ◽  
History Of ◽  
High Bootstrap ◽  
Sex With Men ◽  
Full Length Genome ◽  
Monophyletic Branch ◽  
Hiv 1

Abstract Under the background of the main epidemic HIV strains (CRF01_AE and CRF07_BC) co-circulation in China, more HIV second-generation recombinant (SGR) strains with CRF01_AE and CRF07_BC as the backbone are emerging. In this study, we analyzed the characteristics and evolutionary history of a newly emerging HIV-1 CRF120_0107 composed of CRF01_AE and CRF07_BC based on the near full-length genome (NFLG) in Shenzhen, Guangdong Province, China. NFLG phylogenetic analysis revealed that these sequences formed a distinct monophyletic branch with a high bootstrap value (>90%), distantly related to all known HIV-1 genotypes. Recombination analysis showed that CRF120_0107 was composed of the predominant HIV-1 strains in China: CRF01_AE and CRF07_BC. Further subregional phylogenetic analysis was performed that possible parental lineages of CRF07_BC segments (Ⅰ, Ⅲ, and Ⅴ) belonged to the CRF07_BC men who have sex with men cluster (MSM cluster), other CRF01_AE segments also mainly belonged to MSM Cluster (such as CRF01_AE Cluster 5). Bayesian analysis results inferred that CRF120_0107 placed its emergence in Shenzhen approximately between 2009-2011. The appearance of CRF120_0107 further highlights that more and more HIV-1 SGR strains containing CRF01_AE and CRF07_BC will be more generated frequently and will most likely be more conducive to accelerating the spread of HIV in China. This highlighted it is necessary to monitor MSM high-risk individuals with HIV-1 CRF01_AE and CRF07_BC dual infection to prevent the generation of CRF01_AE/CRF07_BC recombinant strains, thus reducing the possibility of HIV-1 genotype resistance and the complexity of treatment in China.

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