Implementation of HIV Early Infant Diagnosis and HIV Type 1 RNA Viral Load Determination on Dried Blood Spots in Cameroon: Challenges and Propositions

2012 ◽  
Vol 28 (2) ◽  
pp. 176-181 ◽  
Author(s):  
Céline Nguefeu Nkenfou ◽  
Elise Elong Lobé ◽  
Odile Ouwe-Missi-Oukem-Boyer ◽  
Martin Samuel Sosso ◽  
Béatrice Dambaya ◽  
...  
Keyword(s):  
Viral Load ◽  
Dried Blood Spots ◽  
Early Infant Diagnosis ◽  
Blood Spots ◽  
Early Infant ◽  
Hiv Type 1 ◽  
Viral Load Determination

scholarly journals Systematic Review of the Use of Dried Blood Spots for Monitoring HIV Viral Load and for Early Infant Diagnosis

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e86461 ◽  
Author(s):  
Pieter W. Smit ◽  
Kimberly A. Sollis ◽  
Susan Fiscus ◽  
Nathan Ford ◽  
Marco Vitoria ◽  
...  
Keyword(s):  
Systematic Review ◽  
Viral Load ◽  
Dried Blood Spots ◽  
Early Infant Diagnosis ◽  
Hiv Viral Load ◽  
Blood Spots ◽  
Early Infant

scholarly journals Evaluation of the performance of Abbott m2000 and Roche COBAS Ampliprep/COBAS Taqman assays for HIV-1 viral load determination using dried blood spots and dried plasma spots in Kenya

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0179316 ◽  
Author(s):  
Clement Zeh ◽  
Kenneth Ndiege ◽  
Seth Inzaule ◽  
Rebecca Achieng ◽  
John Williamson ◽  
...  
Keyword(s):  
Viral Load ◽  
Dried Blood Spots ◽  
Cobas Taqman ◽  
Blood Spots ◽  
Viral Load Determination ◽  
Hiv 1 ◽  
Roche Cobas

scholarly journals Early infant diagnosis of HIV-1 infection in Luanda, Angola, using a new DNA PCR assay and dried blood spots

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181352 ◽  
Author(s):  
Francisco Martin ◽  
Claudia Palladino ◽  
Rita Mateus ◽  
Anna Bolzan ◽  
Perpétua Gomes ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Early Infant Diagnosis ◽  
Pcr Assay ◽  
Blood Spots ◽  
Early Infant ◽  
Dna Pcr ◽  
Hiv 1

HIV type-1 drug resistance testing on dried blood spots is feasible and reliable in patients who fail antiretroviral therapy in rural Tanzania

2010 ◽  
Vol 15 (7) ◽  
pp. 1003-1009 ◽  
Author(s):  
Asgeir Johannessen ◽  
Mona Holberg-Petersen ◽  
Gunilla Lövgaarden ◽  
Ezra Naman ◽  
Vidar Ormaasen ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antiretroviral Therapy ◽  
Dried Blood Spots ◽  
Resistance Testing ◽  
Blood Spots ◽  
Drug Resistance Testing ◽  
Rural Tanzania ◽  
Hiv Type 1

scholarly journals Early infant diagnosis of HIV-I infection using dried blood spots among children born to seropositive mothers in Federal Medical Centre (FMC) Lokoja

2021 ◽  
Vol 8 (2) ◽  
pp. 136-144
Author(s):  
Aminat Omope Yusuf ◽  
Timothy Olugbenga Ogundeko ◽  
MamzhiSeljul Crown Ramyil ◽  
Catherine Nadabo ◽  
Nkiru Philomena Okoye
Keyword(s):  
Transmission Rate ◽  
Dried Blood Spots ◽  
Antiretroviral Drugs ◽  
Medical Centre ◽  
High Rate ◽  
Type I ◽  
Early Infant Diagnosis ◽  
Transmission Rates ◽  
Blood Spots ◽  
Early Infant

Early diagnosis of Human immunodeficiency virus (HIV) in infants provides a critical opportunity to strengthen follow-up of HIV- exposed children using dried blood spots and assure early access to antiretroviral treatment for infected children. This study aimed to determine the prevalence of HVI-1 infection in infants born to HIV-seropositive mothers. Early infant diagnosis of HIV sub-type I was carried out using on dried blood spots of 286 babies born to HIV-I seropositive mothers attending the Federal Medical Centre, Lokoja - Kogi State, Nigeria, between the months of July to December, 2013. Data obtained was analyzed using Gene Amp PCR System 9700. The overall rate of HIV-I vertical transmission from infected mothers to their babies was 14.5%. High transmission rates 63.5%was seen in babies whose mothers could not get any form of interventions with the least transmission rates seen in babies whose mothers either took HAART or were one form of ARV or the other (0 – 1.0%). Babies who took nevirapine as prophylaxis after delivery had lower rate (1%) of transmission. From the 30 women that mix-fed their babies, 6.7% transmission rate was recorded.Lack of antiretroviral drugs by HIV-I positive pregnant women was found to be associated with high rate of HIV-I transmission (p<0.05). Early intervention of mother to child transmission of HIV-1 infection using Highly Active Antiretroviral Therapy, exclusive breastfeeding practice as well as constant visit to Tertiary Hospitals for counseling and management of HIV infection reduced the rate of infection among the infants born to seropositive mothers.

Performance of Roche CAP/CTM HIV-1 qualitative test version 2.0 using dried blood spots for early infant diagnosis

2016 ◽  
Vol 229 ◽  
pp. 12-15 ◽  
Author(s):  
Sokhna Bousso Gueye ◽  
Halimatou Diop-Ndiaye ◽  
Mamadou Malick Diallo ◽  
Omar Ly ◽  
Aissatou Sow-Ndoye ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Early Infant Diagnosis ◽  
Blood Spots ◽  
Early Infant ◽  
Test Version ◽  
Qualitative Test ◽  
Version 2.0 ◽  
Hiv 1

scholarly journals Comparison of the Gen-Probe Aptima HIV-1 and Abbott HIV-1 qualitative assays with the Roche Amplicor HIV-1 DNA assay for early infant diagnosis using dried blood spots

2014 ◽  
Vol 60 (4) ◽  
pp. 418-421 ◽  
Author(s):  
Julie A.E. Nelson ◽  
J. Tyler Hawkins ◽  
Maria Schanz ◽  
Katie Mollan ◽  
Melissa B. Miller ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Early Infant Diagnosis ◽  
Dna Assay ◽  
Blood Spots ◽  
Early Infant ◽  
Hiv 1

scholarly journals Comparison of Measurements of Autoantibodies to Glutamic Acid Decarboxylase and Islet Antigen-2 in Whole Blood Eluates from Dried Blood Spots Using the RSR-Enzyme Linked Immunosorbent Assay Kits and In-House Radioimmunoassays

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Anders Persson ◽  
Charlotte Becker ◽  
Ida Hansson ◽  
Anita Nilsson ◽  
Carina Törn
Keyword(s):  
Glutamic Acid ◽  
Glutamic Acid Decarboxylase ◽  
Dried Blood Spots ◽  
Enzyme Linked Immunosorbent Assay ◽  
Acid Decarboxylase ◽  
Blood Spots ◽  
And Control ◽  
Islet Antigen ◽  
Prozone Effect

To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%;P=.008). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%;P<.001). Negative or low positive patients and control samples in the RSR-ELISA-IA-2A increased after dilution. Eluates from DBS can readily be used to analyse GADA with the RSR-ELISA, even if low levels of autoantibodies were not detected. Some factor could disturb RSR-ELISA-IA-2A analyses.

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