Genotypic and Phenotypic Cross-Drug Resistance of Harboring Drug-Resistant HIV Type 1 Subtype B′ Strains from Former Blood Donors in Central Chinese Provinces

2010 ◽  
Vol 26 (9) ◽  
pp. 1007-1013 ◽  
Author(s):  
Liying Ma ◽  
Jianghong Huang ◽  
Hui Xing ◽  
Lin Yuan ◽  
Xiaoling Yu ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Blood Donors ◽  
Drug Resistant ◽  
Subtype B ◽  
Hiv Type 1

Drug Resistance and HCV Coinfection in Former Blood Donors Infected with HIV Type 1 in China

2011 ◽  
Vol 27 (8) ◽  
pp. 925-930 ◽  
Author(s):  
Jia Liu ◽  
Xiangmei Chen ◽  
Qing Xie ◽  
Weidong Zhang ◽  
Lai Wei ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Blood Donors ◽  
Hiv Type 1 ◽  
Hcv Coinfection

scholarly journals Prevalence of HIV Drug Resistance Mutations in HIV Type 1 Isolates in Antiretroviral Therapy Naïve Population from Northern India

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
S. Sinha ◽  
H. Ahmad ◽  
R. C. Shekhar ◽  
N. Kumar ◽  
L. Dar ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antiretroviral Therapy ◽  
Resistance Mutations ◽  
Subtype C ◽  
Hiv Drug Resistance ◽  
Northern India ◽  
Subtype B ◽  
Hiv Type 1 ◽  
Hiv 1

Objective. The increased use of antiretroviral therapy (ART) has reduced the morbidity and mortality associated with HIV, adversely leading to the emergence of HIV drug resistance (HIVDR). In this study we aim to evaluate the prevalence of HIVDR mutations in ART-naive HIV-1 infected patients from northern India.Design. Analysis was performed using Viroseq genotyping system based on sequencing of entire protease and two-thirds of the Reverse Transcriptase (RT) region ofpolgene.Results. Seventy three chronic HIV-1 infected ART naïve patients eligible for first line ART were enrolled from April 2006 to August 2008. In 68 patients DNA was successfully amplified and sequencing was done. 97% of HIV-1 strains belonged to subtype C, and one each to subtype A1 and subtype B. The overall prevalence of primary DRMs was 2.9% [2/68, 95% confidence interval (CI), 0.3%–10.2%]. One patient had a major RT mutation M184V, known to confer resistance to lamivudine, and another had a major protease inhibitor (PI) mutation D30N that imparts resistance to nelfinavir.Conclusion. Our study shows that primary HIVDR mutations have a prevalence of 2.9% among ART-naive chronic HIV-1 infected individuals.

The Molecular Epidemiology and Drug Resistance Determination of HIV Type 1 Subtype B Infection in Barbados

2003 ◽  
Vol 19 (4) ◽  
pp. 313-319 ◽  
Author(s):  
Marquita V. Gittens ◽  
William W. Roth ◽  
Timothy Roach ◽  
H. Gene Stringer ◽  
Danuta Pieniazek ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Molecular Epidemiology ◽  
Subtype B ◽  
Hiv Type 1

Progressive emergence of an S153F plus R263K combination of integrase mutations in the proviral DNA of one individual successfully treated with dolutegravir

2020 ◽  
Author(s):  
Hanh T Pham ◽  
Brunna M Alves ◽  
Sunbin Yoo ◽  
Meng A Xiao ◽  
Jing Leng ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Dna Binding ◽  
Drug Resistant ◽  
Strand Transfer ◽  
Viral Genomes ◽  
Proviral Dna ◽  
Viral Loads ◽  
Subtype B ◽  
Transfer Inhibitor ◽  
Integrase Strand Transfer Inhibitor

Abstract Objectives The development of HIV drug resistance against the integrase strand transfer inhibitor dolutegravir is rare. We report here the transient detection, by near full-genome ultradeep sequencing, of minority HIV-1 subtype B variants bearing the S153F and R263K integrase substitutions in the proviral DNA from blood cells of one patient who successfully initiated dolutegravir-based ART, over 24 weeks. Our objective was to study the effects of these substitutions. Methods Strand transfer and DNA-binding activities of recombinant integrase proteins were measured in cell-free assays. Cell-based resistance, infectivity and replicative capacities were measured using molecular clones. Structural modelling was performed to understand experimental results. Results R263K emerged first, followed by the addition of S153F at Week 12. By Week 24, both mutations remained present, but at lower prevalence. We confirmed the coexistence of S153F and R263K on single viral genomes. Combining S153F or S153Y with R263K decreased integration and viral replicative capacity and conferred high levels of drug resistance against all integrase inhibitors. Alone, S153Y and S153F did little to infectivity or dolutegravir resistance. We identified altered DNA binding as a mechanism of resistance. The patient remained with undetectable viral loads at all timepoints. Conclusions Drug-resistant minority variants have often been reported under suppressive ART. Our study adds to these observations by unravelling a progression towards higher levels of resistance through a novel pathway despite continuous undetectable viral loads. Poorly replicative HIV drug-resistant minority proviral variants did not compromise viral suppression in one individual treated with dolutegravir.

scholarly journals Low Prevalence of Transmitted HIV Type 1 Drug Resistance Among Antiretroviral-Naive Adults in a Rural HIV Clinic in Kenya

2013 ◽  
Vol 29 (1) ◽  
pp. 129-135 ◽  
Author(s):  
Amin S. Hassan ◽  
Shalton M. Mwaringa ◽  
Clare A. Obonyo ◽  
Helen M. Nabwera ◽  
Eduard J. Sanders ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Hiv Type 1 ◽  
Low Prevalence

Molecular Phylogenetics of Transmitted Drug Resistance in Newly Diagnosed HIV Type 1 Individuals in Denmark, a Nation-Wide Study

2011 ◽  
Vol 27 (12) ◽  
pp. 1283-1290 ◽  
Author(s):  
Anne Margrethe Audelin ◽  
Jan Gerstoft ◽  
Niels Obel ◽  
Lars Mathiesen ◽  
Alex Laursen ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Molecular Phylogenetics ◽  
Transmitted Drug Resistance ◽  
Newly Diagnosed ◽  
Hiv Type 1

scholarly journals Drug Resistance Mutations and Genetic Diversity in Patients Treated for HIV Type 1 Infection in Rural Care Centers in Togo

2016 ◽  
Vol 06 (03) ◽  
pp. 111-115
Author(s):  
Anoumou Dagnra ◽  
Abla Konou ◽  
Mounerou Salou ◽  
Pascal Kodah ◽  
Damobé Kombate ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Drug Resistance ◽  
Resistance Mutations ◽  
Drug Resistance Mutations ◽  
Hiv Type 1 ◽  
Rural Care

scholarly journals Transmitted drug resistance mutations and subtype diversity among HIV-1 sero-positive voluntary blood donors in Accra, Ghana

2020 ◽  
Author(s):  
Billal Musah Obeng ◽  
Evelyn Yayra Bonney ◽  
Lucy Asamoah-Akuoko ◽  
Nicholas Israel Nii-Trebi ◽  
Gifty Mawuli ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Blood Donors ◽  
Transmitted Drug Resistance ◽  
Nucleotide Sequencing ◽  
Plasma Samples ◽  
Resistance Mutations ◽  
Drug Resistance Mutations ◽  
Subtype B ◽  
Major Drug ◽  
Hiv 1

Abstract Background: Detection of HIV-1 transmitted drug resistance (TDR) and subtype diversity (SD) are public health strategies to assess current HIV-1 regimen and ensure effective therapeutic outcomes of ART among HIV-1 patients. Globally, limited data exist on TDR and SD among blood donors. In this study, drug resistance mutations and subtype diversity among HIV-1 sero-positive blood donors in Accra, Ghana was characterized.Methods: Purposive sampling method was used to collect 81 HIV sero-positive blood samples from the Southern Area Blood Center and confirmed by serology as HIV-1 and/or HIV-2. Viral RNA was only extracted from plasma samples confirmed as HIV-1 positive. Complementary DNA (cDNA) was synthesized using the RNA as a template and subsequently amplified by nested PCR with specific primers. The expected products were verified, purified and sequenced. Neighbor-joining tree with the Kimura’s 2-parameter distances was generated with the RT sequences using Molecular Evolutionary Genetic Analysis version 6.0 (MEGA 6.0).Results: Out of the 81 plasma samples, 60 (74%) were confirmed as HIV-1 sero-positive by INNO-LIA HIVI/II Score kit with no HIV-2 and dual HIV-1/2 infections. The remaining samples, 21 (26%) were confirmed as HIV sero-negative. Of the 60 confirmed positive samples, (32) 53% and (28) 50% were successfully amplified in the RT and PR genes respectively. Nucleotide sequencing of amplified samples revealed the presence of major drug resistance mutations in two (2) samples; E138A in one sample and another with K65R. HIV-1 Subtypes including subtypes A, B, CRF02_AG and CRF09_cpx were found. Conclusion: This study found major drug resistance mutations, E138A and K65R in the RT gene that confer high level resistance to most NNRTIs and NRTI respectively. CRF02_AG was most predominant, the recorded percentage of subtype B and the evolutionary relationship inferred by phylogenetic analysis suggest possible subtype importation. The data obtained would inform the selection of drugs for ART initiation to maximize therapeutic options in drug-naïve HIV-1 patients in Ghana.

Prevalence of HIV Type 1 Antiretroviral Drug Resistance Mutations in Vietnam: A Multicenter Study

2011 ◽  
Vol 27 (7) ◽  
pp. 797-801 ◽  
Author(s):  
Jonathan Dean ◽  
Thu Hong Ta Thi ◽  
Linda Dunford ◽  
Michael J. Carr ◽  
Linh Thuy Nguyen ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Multicenter Study ◽  
Resistance Mutations ◽  
Antiretroviral Drug Resistance ◽  
Drug Resistance Mutations ◽  
Antiretroviral Drug ◽  
Hiv Type 1
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