Structure and Origin of HIV Type 1 DNA in Persistently Infected B Lymphoblastoid Cell Lines

1997 ◽  
Vol 13 (9) ◽  
pp. 751-757 ◽  
Author(s):  
MINGXU GUAN ◽  
GABRIELA TUDOR ◽  
JYH-YUAN YANG ◽  
EARL E. HENDERSON
Keyword(s):  
Cell Lines ◽  
Lymphoblastoid Cell Lines ◽  
Persistently Infected ◽  
Hiv Type 1

Altered Constitutive and Stress-Regulated Heat Shock Protein 27 Expression in HIV Type 1-Infected Cell Lines

1995 ◽  
Vol 11 (6) ◽  
pp. 713-717 ◽  
Author(s):  
BLUMA G. BRENNER ◽  
YUZHEN TAO ◽  
ELEENA PEARSON ◽  
ISRAEL REMER ◽  
MARK A. WAINBERG
Keyword(s):  
Heat Shock ◽  
Infected Cell ◽  
Heat Shock Protein ◽  
Cell Lines ◽  
Heat Shock Protein 27 ◽  
Hiv Type 1

Alteration of CD44 Expression in HIV Type 1-Infected T Cell Lines

1996 ◽  
Vol 12 (17) ◽  
pp. 1615-1622 ◽  
Author(s):  
VALÉRIE GIORDANENGO ◽  
MARTINE LIMOUSE ◽  
ALAIN DOGLIO ◽  
JOSETTE LESIMPLE ◽  
JEAN-CLAUDE LEFEBVRE
Keyword(s):  
T Cell ◽  
Cell Lines ◽  
Cd44 Expression ◽  
Hiv Type 1 ◽  
T Cell Lines

Replication of HIV Type 1 in Rabbit Cell Lines Is Not Limited by Deficiencies intat, rev, or Long Terminal Repeat Function

1995 ◽  
Vol 11 (12) ◽  
pp. 1487-1493 ◽  
Author(s):  
SUNGAE CHO ◽  
THOMAS J. KINDT ◽  
TONG-MAO ZHAO ◽  
SANSANA SAWASDIKOSOL ◽  
BISHOP F. HAGUE
Keyword(s):  
Long Terminal Repeat ◽  
Cell Lines ◽  
Terminal Repeat ◽  
Hiv Type 1

Localization of constituent proteins of HIV Type 1 (HIV-1)

1990 ◽  
Vol 48 (3) ◽  
pp. 340-341
Author(s):  
Toshiyuki Goto ◽  
Chizuko Morita ◽  
Taisuke Ashina ◽  
Kazuyoshi Ikuta ◽  
Shiro Kato ◽  
...  
Keyword(s):  
Cell Lines ◽  
Uranyl Acetate ◽  
Mouse Serum ◽  
Infected Cells ◽  
Hiv Type 1 ◽  
Lowicryl K4m ◽  
Specific Reactions ◽  
Phosphate Buffered Saline ◽  
Hiv 1

The localization of the constituent proteins of HIV-1 in the virions and HIV-1-infected cells was examined by indirect immuno-gold labeling with monoclonal antibodies (MoAb) to gag proteins (p18 and p24/p53), to elucidate viral formation of HIV.Persistently HIV-1 (HTLV—IIIB,LAV-1)-infected MT-4 and MOLT-4 cell lines and their cloned cell lines were used as infected cells. Mouse MoAb against HIV-1 gag p18 (V17) and p24/p53 (V107) were used. The cells were fixed with 0.5-1% glutar-aldehyde in phosphate-buffered saline (pH 7.2), dehydrated in ethanol and embedded in epoxy (at 45° Cor 60°C) or Lowicryl K4M resin (at −30°C). The sections were incubated in MoAb at room temperature for 1 h and then incubated in anti-mouse goat IgG conjugated with gold (IgG-gold, 5 or 15 nm; Janssen) for 40 min. After being washed, the sections were stained with uranyl acetate and lead citrate, and were observed in an electron microscope with a tilting apparatus.The spećific reactions with V17 and V107 were detected on HIV-1 particles and in the infected cells. No reactivity was noted between uninfected control cells and the MoAb, or between the infected cells and normal mouse serum. More than 97% of the HIV-1 particles embedded in epoxy resinat 60°C were labeled with gold after exclusion of the HIV particles that were attached to supporting film or completely embedded in the section (Fig. 1). Increased labeling was observed with Lowicryl (Fig. 2)and epoxy resin embedding at 45°C.

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