scholarly journals Kinetic Measurements Using Flow Cytometry: New Methods for Monitoring Intracellular Processes

2012 ◽  
Vol 10 (1) ◽  
pp. 97-104 ◽  
Author(s):  
Gergő Mészáros ◽  
Balázs Szalay ◽  
Gergely Toldi ◽  
Ambrus Kaposi ◽  
Barna Vásárhelyi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Kinetic Measurements ◽  
New Methods

Time: a new parameter for kinetic measurements in flow cytometry

Science ◽  
1980 ◽  
Vol 207 (4427) ◽  
pp. 199-201 ◽  
Author(s):  
J. Martin ◽  
D. Swartzendruber
Keyword(s):  
Flow Cytometry ◽  
Kinetic Measurements

A novel flow cytometry–based platelet aggregation assay

Blood ◽  
2013 ◽  
Vol 121 (10) ◽  
pp. e70-e80 ◽  
Author(s):  
Iris M. De Cuyper ◽  
Marjolein Meinders ◽  
Edith van de Vijver ◽  
Dirk de Korte ◽  
Leendert Porcelijn ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Platelet Aggregation ◽  
Animal Models ◽  
Small Volume ◽  
Aggregation Assay ◽  
Kinetic Measurements ◽  
Experimental Animal Models ◽  
Single Receptor ◽  
Key Points ◽  
Platelet Aggregation Assay

Key Points FCA is a novel flow cytometry–based platelet aggregation assay that allows single receptor analysis in small volume/thrombocytopenic samples FCA facilitates platelet studies in experimental animal models even during gestation and allows kinetic measurements in individual animals

An easy-to-build-timer for kinetic measurements in flow cytometry

Cytometry ◽  
1984 ◽  
Vol 5 (6) ◽  
pp. 648-651 ◽  
Author(s):  
Tom Beumer ◽  
Hans Lenssinck ◽  
Arie Pennings ◽  
Clemens Haanen
Keyword(s):  
Flow Cytometry ◽  
Kinetic Measurements

Chapter 32 On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements

1994 ◽  
pp. 509-525 ◽  
Author(s):  
Kees Nooter ◽  
Hans Herweijer ◽  
Richard R. Jonker ◽  
Ger J. van den Engh
Keyword(s):  
Flow Cytometry ◽  
Kinetic Measurements ◽  
Line Flow ◽  
On Line

Chapter 52 On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements

1990 ◽  
pp. 631-645 ◽  
Author(s):  
Kees Nooter ◽  
Hans Herweijer ◽  
Richard Jonker ◽  
Ger Van Den Engh
Keyword(s):  
Flow Cytometry ◽  
Kinetic Measurements ◽  
Line Flow ◽  
On Line

scholarly journals New Technologies for Use in Toxicology Studies: Monitoring the Effects of Xenobiotics on Immune Function

1990 ◽  
Vol 9 (3) ◽  
pp. 303-317 ◽  
Author(s):  
J. Paul Robinson ◽  
R.W. Pfeifer
Keyword(s):  
Flow Cytometry ◽  
New Technologies ◽  
Single Cells ◽  
Specific Cell ◽  
Kinetic Measurements ◽  
New Developments ◽  
Cell Functions ◽  
Alternative Approach ◽  
In Vitro Systems

New developments in flow cytometry are now being applied in toxicology studies. There are several reasons for using this technology. First, techniques are well characterized to measure functional parameters of single cells. Such measurements can be directly related to perturbations by xenobiotics, cell-mediated immune responses, or trauma. Second, there is a clear indication for movement toward in vitro systems as highly objective assessments of toxicologic interactions. By measuring specific cell functions at the single cell level, it is possible to define a range of normal responses. More importantly, a multiparametric analysis can be performed with flow cytometry and parameters can be directly related to one another. Furthermore, kinetic measurements can be made, providing vital clues to the mechanisms of actions of drugs or chemicals on functions of specific cell populations. Major advantages of this approach are that studies can be performed on very small volumes of blood, body fluid, or cell culture lines and it is not necessary to isolate pure populations of cells to perform these assays. We believe that this alternative approach in toxicology will provide valuable information unobtainable by traditional means.

Replication of Wet Objects and Cells

1974 ◽  
Vol 32 ◽  
pp. 102-103
Author(s):  
S. Basu ◽  
D. F. Parsons
Keyword(s):  
Water Vapor Pressure ◽  
High Vacuum ◽  
Polystyrene Latex ◽  
Vacuum System ◽  
Saturated Water Vapor ◽  
Replication Method ◽  
New Methods ◽  
Saturated Water ◽  
Water Vapors ◽  
Intermediate Chamber

We are approaching the invasiveness of cancer cells from the studies of their wet surface morphology which should distinguish them from their normal counterparts. In this report attempts have been made to provide physical basis and background work to a wet replication method with a differentially pumped hydration chamber (Fig. 1) (1,2), to apply this knowledge for obtaining replica of some specimens of known features (e.g. polystyrene latex) and finally to realize more specific problems and to improvize new methods and instrumentation for their rectification. In principle, the evaporant molecules penetrate through a pair of apertures (250, 350μ), through water vapors and is, then, deposited on the specimen. An intermediate chamber between the apertures is pumped independently of the high vacuum system. The size of the apertures is sufficiently small so that full saturated water vapor pressure is maintained near the specimen.

A Freeze Shadow Technique for the Preparation of Soft Paint Latexes for Electron Microscopy

1977 ◽  
Vol 35 ◽  
pp. 314-315
Author(s):  
Earl R. Walter ◽  
Glen H. Bryant
Keyword(s):  
Sample Preparation ◽  
High Vacuum ◽  
Vacuum System ◽  
Latex Particles ◽  
New Methods ◽  
Diffusion Pump ◽  
Film Forming ◽  
Routine Basis ◽  
Distribution Studies ◽  
Preparation Techniques

With the development of soft, film forming latexes for use in paints and other coatings applications, it became desirable to develop new methods of sample preparation for latex particle size distribution studies with the electron microscope. Conventional latex sample preparation techniques were inadequate due to the pronounced tendency of these new soft latex particles to distort, flatten and fuse on the substrate when they dried. In order to avoid these complications and obtain electron micrographs of undistorted latex particles of soft resins, a freeze-dry, cold shadowing technique was developed. The method has now been used in our laboratory on a routine basis for several years.The cold shadowing is done in a specially constructed vacuum system, having a conventional mechanical fore pump and oil diffusion pump supplying vacuum. The system incorporates bellows type high vacuum valves to permit a prepump cycle and opening of the shadowing chamber without shutting down the oil diffusion pump. A baffeled sorption trap isolates the shadowing chamber from the pumps.

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