Inflammatory Pathway Analysis Using a High Content Screening Platform

2005 ◽  
Vol 3 (3) ◽  
pp. 261-271 ◽  
Author(s):  
Malene Bertelsen ◽  
Annika Sanfridson
Keyword(s):  
Pathway Analysis ◽  
High Content Screening ◽  
Inflammatory Pathway ◽  
Screening Platform

Predictive Modeling and Biomechanical Microengineering of Mesenchymal Stem Cells: A High Content Screening Platform to Enhance Lineage Specific Differentiation

2013 ◽  
Author(s):  
Amit Paul ◽  
David Franz ◽  
Sumaira Yahya ◽  
Shan Sun ◽  
Michael Cho
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Predictive Modeling ◽  
Stem Cell Differentiation ◽  
High Content Screening ◽  
Cell Behavior ◽  
Functional Changes ◽  
Screening Platform

Recent evidence suggests that stem cell differentiation can be regulated by modulation of the cell’s biomechanics. The cytoskeletal structures and arrangements in stem cells undergoing differentiation are dramatically altered, and these alterations vary by lineage. The complexity of events associated with the transformation of these precursor cells leaves many questions unanswered about morphological, structural, proteomic, and functional changes in differentiating stem cells. A thorough understanding of stem cell behavior, both experimentally and computationally, would allow for the development of more effective approaches to the expansion of stem cells in vitro and for the regulation of their commitment to a specific phenotype.

scholarly journals Activation and Translocation of Glucokinase in Rat Primary Hepatocytes Monitored by High Content Image Analysis

2008 ◽  
Vol 13 (9) ◽  
pp. 837-846 ◽  
Author(s):  
Michael Wolff ◽  
Stefan G. Kauschke ◽  
Susanne Schmidt ◽  
Ralf Heilker
Keyword(s):  
Regulatory Protein ◽  
Rat Hepatocytes ◽  
High Content Screening ◽  
Metabolic Enzyme ◽  
Screening Assay ◽  
Extracellular Medium ◽  
Rat Primary Hepatocytes ◽  
Translocation Assay ◽  
Low Glucose ◽  
Screening Platform

In the liver, glucokinase (GK) regulatory protein (GKRP) negatively modulates the metabolic enzyme GK by locking it in an inactive state in the nucleus. Here, the authors established a high content screening assay in the 384-well microplate format to measure the nucleus-to-cytoplasm translocation of GK by reagents that destabilize the interaction between GK and GKRP. As a cellular model system, primary rat hepatocytes endogenously expressing both GK and GKRP at physiological levels were used. The GK translocation assay was robust, displayed limited day-to-day variability, and delivered good Z′ statistics. The increase of the glucose concentration in the extracellular medium from a low glucose situation (2.8 mM) to beyond its physiological set point value of 5 mM was found to drive GK from the nucleus into the cytoplasm. Likewise, both fructose (converted intracellularly into fructose-1-phosphate) and a known allosteric GK activator were found to induce the export of GK from the nucleus and to synergistically enhance the effects of medium or high glucose concentrations with respect to GK translocation. Transfer of the high content screening format to a semiautomated medium throughput screening platform enabled the profiling of large compound numbers with respect to allosteric activation of GK. ( Journal of Biomolecular Screening 2008:837-846)

scholarly journals Designs and Concept Reliance of a Fully Automated High-Content Screening Platform

2012 ◽  
Vol 17 (5) ◽  
pp. 359-369 ◽  
Author(s):  
Constantin Radu ◽  
Hosna Sana Adrar ◽  
Ab Alamir ◽  
Ian Hatherley ◽  
Trung Trinh ◽  
...  
Keyword(s):  
High Content Screening ◽  
Screening Platform

scholarly journals High-Content Screening for Biofilm Assays

2010 ◽  
Vol 15 (7) ◽  
pp. 748-754 ◽  
Author(s):  
Fubing Peng ◽  
Eric M. V. Hoek ◽  
Robert Damoiseaux
Keyword(s):  
High Throughput ◽  
Bacterial Adhesion ◽  
Biofilm Formation ◽  
High Throughput Screening ◽  
High Content Screening ◽  
Cross Linking ◽  
Coating Film ◽  
Coating Films ◽  
Engineered Surfaces ◽  
Screening Platform

The authors describe a novel high-throughput screening platform that provides rapid, reliable, quantitative assessment of biofilm formation and removal on engineered surfaces. Unlike traditional biofilm assays based on plate readers, this assay platform is based on high-content screening, which allows for multiplexing to simultaneously quantify the number of bacterial adhesions per unit area and the viability of adhered cells using fluorescent dye combinations. This platform is fully automated and has a throughput of more than 10,000 wells per day. The authors used this platform to examine the influence of different assay buffer systems on bacterial adhesion, viability, and removal on cross-linked polyvinyl alcohol coating films synthesized directly onto the bottoms of 384-well plates. The results indicated that water chemistry, bacteria cell type, and film chemistry combine to govern biofilm formation. In general, both reversible and irreversible bacterial adhesion increased with the extent of cross-linking in coating films, which correlates strongly with coating film cross-linking degree and hydrophobicity, which is closely related. The high-throughput platform offers a powerful tool for rapid evaluation of fouling-resistant coating films in addition to elucidation of fundamental mechanisms governing bacterial adhesion.

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