Osteoinduction by Bone Morphogenetic Protein 2-Expressing Adenoviral Vector: Application of Biomaterial to Mask the Host Immune Response

2004 ◽  
Vol 15 (7) ◽  
pp. 659-668 ◽  
Author(s):  
Junya Sonobe ◽  
Yasunori Okubo ◽  
Shinji Kaihara ◽  
Shin-ichi Miyatake ◽  
Kazuhisa Bessho
Keyword(s):  
Immune Response ◽  
Bone Morphogenetic Protein ◽  
Adenoviral Vector ◽  
Host Immune Response ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

Bone morphogenetic protein 2 stimulates chondrogenesis of equine synovial membrane-derived progenitor cells

2016 ◽  
Vol 29 (05) ◽  
pp. 378-385 ◽  
Author(s):  
Yuwen Chen ◽  
Evelyn Caporali ◽  
Matthew Stewart
Keyword(s):  
Progenitor Cells ◽  
Bone Morphogenetic Protein ◽  
Synovial Membrane ◽  
Adenoviral Vector ◽  
Collagen Type ◽  
Bone Morphogenetic Protein 2 ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Potent Inducer ◽  
Morphogenetic Protein

SummaryObjectives: Bone morphogenetic protein 2 (BMP-2) is critical for skeletal and cartilage development, homeostasis and repair. This study was conducted to clone and characterize equine BMP-2, develop expression constructs for equine BMP-2, and to determine whether BMP-2 can stimulate chondrogenesis of equine synovial membrane-derived progenitor cells (SMPC).Methods: Equine BMP-2 cDNA was amplified from chondrocyte RNA, and then transferred into an expression plasmid and adenoviral vector. Effective expression of equine BMP-2 was confirmed using a BMP reporter cell line. SMPC were isolated from synovium, expanded through two passages and transferred to chondrogenic cultures, with recombinant human (rh) transforming growth factor beta 1 (TGF-[uni03B2]1) or rhBMP-2. Chondro-genesis was assessed by up-regulation of collagen types II and X, and aggrecan mRNA, secretion of collagen type II protein and sulfated glycosaminoglycans (sGAG), and by alkaline phosphatase induction. Chondrogenic stimulation of SMPC by the equine BMP-2 adenovirus was assessed by sGAG secretion and histology.Results: The mature equine BMP-2 peptide is identical to human and murine peptides. Recombinant human BMP-2 and TGF-[uni03B2]1 stimulated equivalent amounts of collagen type II protein in SMPC pellets, but sGAG secretion was doubled by BMP-2. Neither factor stimulated hypertrophic marker expression. The equine BMP-2 adenoviral vector induced chondrogenesis comparably to rhBMP-2 protein, with no indication of hypertrophy.Clinical significance: Bone morphogenetic protein 2 is a potent inducer of SMPC nonhypertrophic chondrogenesis, supporting the use of this combination for articular cartilage repair applications.

The time course study of osteoinduction by bone morphogenetic protein-2 via adenoviral vector

Life Sciences ◽  
2001 ◽  
Vol 70 (3) ◽  
pp. 325-336 ◽  
Author(s):  
Yasunori Okubo ◽  
Kazuhisa Bessho ◽  
Kazuma Fujimura ◽  
Shinji Kaihara ◽  
Tadahiko Iizuka ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Adenoviral Vector ◽  
Time Course ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Time Course Study

In Vitro and in Vivo Studies of a Bone Morphogenetic Protein-2 Expressing Adenoviral Vector

2001 ◽  
Vol 83 ◽  
pp. S1-99-S1-104 ◽  
Author(s):  
Yasunori Okubo ◽  
Kazuhisa Bessho ◽  
Kazuma Fujimura ◽  
Tadahiko Iizuka ◽  
Shin-ichi Miyatake
Keyword(s):  
Bone Morphogenetic Protein ◽  
Adenoviral Vector ◽  
Bone Morphogenetic Protein 2 ◽  
In Vivo Studies ◽  
Morphogenetic Protein

Osteoinduction by Bone Morphogenetic Protein-2 via Adenoviral Vector under Transient Immunosuppression

2000 ◽  
Vol 267 (1) ◽  
pp. 382-387 ◽  
Author(s):  
Yasunori Okubo ◽  
Kazuhisa Bessho ◽  
Kazuma Fujimura ◽  
Tadahiko Iizuka ◽  
Shin-Ichi Miyatake
Keyword(s):  
Bone Morphogenetic Protein ◽  
Adenoviral Vector ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

In Vivo Endochondral Bone Formation Using a Bone Morphogenetic Protein 2 Adenoviral Vector

1999 ◽  
Vol 10 (13) ◽  
pp. 2245-2253 ◽  
Author(s):  
Tord D. Alden ◽  
Debra D. Pittman ◽  
Gerald R. Hankins ◽  
Elisa J. Beres ◽  
Johnathan A. Engh ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Adenoviral Vector ◽  
Bone Morphogenetic Protein 2 ◽  
Endochondral Bone Formation ◽  
Endochondral Bone ◽  
Morphogenetic Protein
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