Oxygen Consumption of Lampreys, with Special Reference to Metamorphosis and Phylogenetic Position

1946 ◽  
Vol 19 (4) ◽  
pp. 365-374 ◽  
Author(s):  
W. James Leach
Author(s):  
Ancel Keys ◽  
E. H. Christensen ◽  
August Krogh

Studies are reported of the behaviour of stored sea-water with regard to oxygen, ammonia, and bacteria content in relation to the conditions of storage and to the effect of various filtration procedures.When sea-water is sterilised by filtration and stored in the dark, the oxygen content remains constant or diminishes only by less than 0·07c.c. per litre in several hundred hours.In non-sterile experiments there is always an oxygen consumption roughly parallel to a bacterial multiplication which begins very suddenly after collection of the water. These effects are greatest in “raw” water, less in paper-filtered water and least in water which is doubly filtered.


Circulation ◽  
1960 ◽  
Vol 22 (4) ◽  
pp. 559-562 ◽  
Author(s):  
GEORGE G. ROWE ◽  
GEORGE M. MAXWELL ◽  
CESAR A. CASTILLO ◽  
JOHN H. HUSTON ◽  
CHARLES W. CRUMPTON

1979 ◽  
Vol 25 (4) ◽  
pp. 531-534 ◽  
Author(s):  
S Meites ◽  
K Saniel-Banrey

Abstract Of several preservatives we tested, a mixture of "isotonic" sodium fluoride and sodium iodoacetate (17.7 and 5.0 g/L, respectively) best inhibits glucose loss from blood collected from the newborn. However, the inhibition is incomplete. Twenty microliters of blood is diluted with 100 microliter of preserving solution. After centrifugation, the glucose in 100 microliter of prewarmed supernate (diluted plasma) is measured in a Beckman Glucose Analyzer, based on the action of a glucose reagent and the rate of oxygen consumption. The method, in effect, determines the glucose in whole blood, except for about 5% of the total that remains in the erythrocytes. Glucose is distributed in blood according to the water content of cells and plasma. From concentrations found in cells and plasma, we can calculate the value for glucose in whole blood with an accuracy of 95.2%. The modified method we describe shows acceptable precision, as judged from within-run and day-to-day CV--generally well below 5% for each.


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