Assessment of genetic diversity and structure among populations of Epioblasma triquetra in the Laurentian Great Lakes drainage

2019 ◽  
Vol 38 (3) ◽  
pp. 527-542 ◽  
Author(s):  
Caitlin E. Beaver ◽  
Daelyn A. Woolnough ◽  
David T. Zanatta
2020 ◽  
Vol 98 (8) ◽  
pp. 551-556
Author(s):  
N.M. VanTassel ◽  
C.E. Beaver ◽  
D.A. Watkinson ◽  
T.J. Morris ◽  
D.T. Zanatta

Our study documents and analyzes the absence of genetic diversity and structure of the Mapleleaf (Quadrula quadrula (Rafinesque, 1820)) (Bivalvia: Unionidae) mussel in the Lake Winnipeg, Assiniboine River, and Red River drainages (Manitoba, Canada). Previous studies have revealed patterns of genetic diversity and structure in the Mississippi and Ohio river drainages, as well as in the Laurentian Great Lakes drainage. Genotypes from six variable microsatellite loci showed that the Q. quadrula population in Manitoba was significantly differentiated from the population in the Great Lakes drainage (Ontario, Canada), supporting the existence of two Designatable Units in Canada. Conversely, there was no evidence of genetic structure within the sampled range of Q. quadrula in Manitoba. The lack of genetic structure in Q. quadrula across its distribution in Manitoba reflected its post-glacial history and use of a vagile host and necessitates that efforts should be made to ensure connectivity and maintain gene flow across the region. Given that the evidence suggests that Manitoba Q. quadrula belong to a single genetic population, movement of hatchery-propagated juvenile Q. quadrula, adult Q. quadrula, or glochidia-carrying host catfish sourced from any location in Manitoba could be used to augment declining populations or at-risk locations in Manitoba.


2009 ◽  
Vol 75 (11) ◽  
pp. 3598-3604 ◽  
Author(s):  
Christopher J. Allender ◽  
Gary R. LeCleir ◽  
Johanna M. Rinta-Kanto ◽  
Randall L. Small ◽  
Michael F. Satchwell ◽  
...  

ABSTRACT While multiple phylogenetic markers have been used in the culture-independent study of microcystin-producing cyanobacteria, in only a few instances have multiple markers been studied within individual cells, and in all cases these studies have been conducted with cultured isolates. Here, we isolate and evaluate large DNA fragments (>6 kb) encompassing two genes involved in microcystin biosynthesis (mcyA2 and mcyB1) and use them to identify the source of gene fragments found in water samples. Further investigation of these gene loci from individual cyanobacterial cells allowed for improved analysis of the genetic diversity within microcystin producers as well as a method to predict microcystin variants for individuals. These efforts have also identified the source of the novel mcyA genotype previously termed Microcystis-like that is pervasive in the Laurentian Great Lakes and they predict the microcystin variant(s) that it produces.


Author(s):  
John Lekki ◽  
R. Anderson ◽  
Q.-V. Nguyen ◽  
J. Demers ◽  
J. Flatico ◽  
...  

2017 ◽  
Author(s):  
John W. Johnston ◽  
◽  
Erin P. Argyilan ◽  
Steve J. Baedke ◽  
Sean Morrison ◽  
...  

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Fehintola V. Ajogbasile ◽  
Adeyemi T. Kayode ◽  
Paul E. Oluniyi ◽  
Kazeem O. Akano ◽  
Jessica N. Uwanibe ◽  
...  

Abstract Background Malaria remains a public health burden especially in Nigeria. To develop new malaria control and elimination strategies or refine existing ones, understanding parasite population diversity and transmission patterns is crucial. Methods In this study, characterization of the parasite diversity and structure of Plasmodium falciparum isolates from 633 dried blood spot samples in Nigeria was carried out using 12 microsatellite loci of P. falciparum. These microsatellite loci were amplified via semi-nested polymerase chain reaction (PCR) and fragments were analysed using population genetic tools. Results Estimates of parasite genetic diversity, such as mean number of different alleles (13.52), effective alleles (7.13), allelic richness (11.15) and expected heterozygosity (0.804), were high. Overall linkage disequilibrium was weak (0.006, P < 0.001). Parasite population structure was low (Fst: 0.008–0.105, AMOVA: 0.039). Conclusion The high level of parasite genetic diversity and low population structuring in this study suggests that parasite populations circulating in Nigeria are homogenous. However, higher resolution methods, such as the 24 SNP barcode and whole genome sequencing, may capture more specific parasite genetic signatures circulating in the country. The results obtained can be used as a baseline for parasite genetic diversity and structure, aiding in the formulation of appropriate therapeutic and control strategies in Nigeria.


Author(s):  
Edward S. Rutherford ◽  
Hongyan Zhang ◽  
Yu‐Chun Kao ◽  
Doran M. Mason ◽  
Ali Shakoor ◽  
...  

Author(s):  
Euan D. Reavie ◽  
Meijun Cai ◽  
Carsten Meyer-Jacob ◽  
John P. Smol ◽  
Josef P. Werne

Inland Waters ◽  
2021 ◽  
pp. 1-15
Author(s):  
Elisa Calamita ◽  
Sebastiano Piccolroaz ◽  
Bruno Majone ◽  
Marco Toffolon

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