Acinetobacter baumannii: Association between Environmental Contamination of Patient Rooms and Occupant Status

2013 ◽  
Vol 34 (5) ◽  
pp. 517-520 ◽  
Author(s):  
L. Silvia Munoz-Price ◽  
Nicholas Namias ◽  
Timothy Cleary ◽  
Yovanit Fajardo-Aquino ◽  
Dennise DePascale ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Acinetobacter Baumannii ◽  
Environmental Contamination ◽  
Gel Electrophoresis ◽  
Odds Ratio ◽  
Pulsed Field Gel Electrophoresis ◽  
Carrier Status ◽  
Environmental Isolates ◽  
Pulsed Field

We aimed to determine the association between the presence ofAcinetobacter baumanniiin patient rooms and the carrier status of the occupants. Fifty-six (39%) of 143 rooms withA. baumannii– positive patients had results positive forA. baumannii.Only 49 (10%) of 485 rooms withA. baumannii-negative patients were positive (odds ratio, 5.72 [95% confidence interval, 3.66–8.96];P< .0001). Clinical and environmental isolates shared pulsed-field gel electrophoresis patterns.

scholarly journals Pulsed-Field Gel Electrophoresis and PCR Characterization of Environmental Vibrio parahaemolyticus Strains of Different Origins

2011 ◽  
Vol 77 (17) ◽  
pp. 6301-6304 ◽  
Author(s):  
E. Suffredini ◽  
C. Lopez-Joven ◽  
L. Maddalena ◽  
L. Croci ◽  
A. Roque
Keyword(s):  
Gel Electrophoresis ◽  
Vibrio Parahaemolyticus ◽  
Genetic Relationships ◽  
Pulsed Field Gel Electrophoresis ◽  
Environmental Isolates ◽  
Content Type ◽  
Pulsed Field ◽  
Intraspecies Variability ◽  
Different Origins

ABSTRACTThe present study used pulsed-field gel electrophoresis (PFGE) characterization to examine the intraspecies variability and genetic relationships among environmental isolates ofVibrio parahaemolyticusfrom different European countries. This is first study performed on environmentalV. parahaemolyticusthat included more than one European country.

scholarly journals Australian Isolates of Legionella longbeachae Are Not a Clonal Population

1999 ◽  
Vol 37 (10) ◽  
pp. 3249-3254 ◽  
Author(s):  
J. C. Montanaro-Punzengruber ◽  
L. Hicks ◽  
W. Meyer ◽  
G. L. Gilbert
Keyword(s):  
Genetic Variability ◽  
Legionella Pneumophila ◽  
Gel Electrophoresis ◽  
Type Strain ◽  
Pulsed Field Gel Electrophoresis ◽  
Epidemiological Investigation ◽  
Environmental Isolates ◽  
Clonal Population ◽  
Pulsed Field ◽  
Major Cluster

Legionella longbeachae is almost as frequent a cause of legionellosis in Australia as Legionella pneumophila, but epidemiological investigation of possible environmental sources and clinical cases has been limited by the lack of a discriminatory subtyping method. The purpose of this study was to examine the genetic variability among Australian isolates of L. longbeachaeserogroup 1. Pulsed-field gel electrophoresis (PFGE) ofSfiI fragments revealed three distinct pulsotypes among 57 clinical and 11 environmental isolates and the ATCC control strains of L. longbeachae serogroups 1 and 2. Each pulsotype differed by four bands, corresponding to <65% similarity. A clonal subgroup within each pulsotype was characterized by >88% similarity. The largest major cluster was pulsotype A, which included 43 clinical isolates and 9 environmental isolates and was divided into five subgroups. Pulsotypes B and C comprised smaller numbers of clinical and environmental isolates, which could each be further divided into three subgroups. The ATCC type strain of L. longbeachae serogroup 1 was classified as pulsotype B, subtype B3, while the ATCC type strain of L. longbeachae serogroup 2 was identified as a different pulsotype, LL2. SfiI macrorestriction analysis followed by PFGE showed that the AustralianL. longbeachae strains are not a single clonal population as previously reported.

scholarly journals Identification of 16S rRNA Methylase-Producing Acinetobacter baumannii Clinical Strains in North America

2007 ◽  
Vol 51 (11) ◽  
pp. 4209-4210 ◽  
Author(s):  
Yohei Doi ◽  
Jennifer M. Adams ◽  
Kunikazu Yamane ◽  
David L. Paterson
Keyword(s):  
North America ◽  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
Gel Electrophoresis ◽  
Medical Center ◽  
Pulsed Field Gel Electrophoresis ◽  
Aminoglycoside Resistance ◽  
Pulsed Field ◽  
Clinical Strains ◽  
16S Rrna Methylase

ABSTRACT Five highly amikacin-resistant Acinetobacter baumannii isolates were collected at a medical center in Pennsylvania. The aminoglycoside resistance was due to the production of the 16S rRNA methylase ArmA. Two of the isolates coproduced OXA-23 β-lactamase and were highly resistant to carbapenems as well. The isolates were genetically closely related by pulsed-field gel electrophoresis.

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