scholarly journals Progressive Telomere Shortening of Epstein‐Barr Virus–Specific Memory T Cells during HIV Infection: Contributor to Exhaustion?

2008 ◽  
Vol 198 (9) ◽  
pp. 1353-1357 ◽  
Author(s):  
Debbie van Baarle ◽  
Nening M. Nanlohy ◽  
Sigrid Otto ◽  
Fiona J. Plunkett ◽  
Jean M. Fletcher ◽  
...  
Keyword(s):  
T Cells ◽  
Hiv Infection ◽  
Epstein Barr Virus ◽  
Memory T Cells ◽  
Telomere Shortening ◽  
Barr Virus ◽  
Specific Memory ◽  
Epstein Barr

scholarly journals Loss of EBNA1-specific memory CD4+ and CD8+ T cells in HIV-infected patients progressing to AIDS-related non-Hodgkin lymphoma

Blood ◽  
2005 ◽  
Vol 106 (9) ◽  
pp. 3166-3174 ◽  
Author(s):  
Erwan Piriou ◽  
Karel van Dort ◽  
Nening M. Nanlohy ◽  
Marinus H. J. van Oers ◽  
Frank Miedema ◽  
...  
Keyword(s):  
T Cells ◽  
Hiv Infection ◽  
Cd8 T Cells ◽  
Cd4 T Cells ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Non Hodgkin Lymphoma ◽  
Slow Progressors ◽  
Specific Memory ◽  
Epstein Barr

AbstractWe previously observed a loss of Epstein-Barr virus (EBV)–specific CD8+ T cells in subjects progressing to EBV-related non-Hodgkin lymphoma (NHL), correlating with loss of CD4+ T cells. The aim of the present study was to determine the role of EBV-specific CD4+ T cells in the development of NHL during chronic HIV infection. To this end, CD4+ and CD8+ memory T cells, capable of both proliferation and subsequent interferon γ (IFNγ) production, directed against a latent (Epstein-Barr virus nuclear antigen 1 [EBNA1]) and a lytic (BamH fragment Z left frame 1 [BZLF1]) EBV antigen were studied longitudinally in 9 progressors to NHL, 4 progressors to non–EBV-related AIDS, and 4 slow progressors to AIDS. In all 3 groups we observed a decline of EBV-specific memory CD4+ and CD8+ T-cell responses during HIV infection. However, whereas latent antigen EBNA1-specific CD4+ T cells were lost well before diagnosis in all subjects who developed an AIDS-related NHL (and EBNA1-specific CD8+ T cells were significantly lower compared with the other groups), these cells were better preserved in progressors to non–EBV-related disease and slow progressors. Loss of EBNA1-specific T-cell immunity thus might be important for progression to NHL. Interestingly, BZLF1-specific T cells were not lost in all progressors to NHL, suggesting a different function of these cells in the surveillance of EBV-infected B cells.

scholarly journals Detection of Epstein-Barr virus-specific memory CD4+T cells using a peptide-based cultured enzyme-linked immunospot assay

Immunology ◽  
2013 ◽  
Vol 139 (4) ◽  
pp. 533-544 ◽  
Author(s):  
Sandra A. Calarota ◽  
Antonella Chiesa ◽  
Paola Zelini ◽  
Giuditta Comolli ◽  
Lorenzo Minoli ◽  
...  
Keyword(s):  
T Cells ◽  
Cd4 T Cells ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Specific Memory ◽  
Epstein Barr ◽  
Memory Cd4 T Cells

The flow cytometric analysis of telomere length in antigen-specific CD8+ T cells during acute Epstein-Barr virus infection

Blood ◽  
2001 ◽  
Vol 97 (3) ◽  
pp. 700-707 ◽  
Author(s):  
Fiona J. Plunkett ◽  
Maria Vieira D. Soares ◽  
Nicola Annels ◽  
Andrew Hislop ◽  
Kamal Ivory ◽  
...  
Keyword(s):  
T Cells ◽  
Telomere Length ◽  
Cd8 T Cells ◽  
Epstein Barr Virus ◽  
Replicative Senescence ◽  
Telomere Maintenance ◽  
Epstein Barr Virus Infection ◽  
Telomere Shortening ◽  
Barr Virus ◽  
Epstein Barr

Abstract Acute infectious mononucleosis (AIM) induced by Epstein-Barr virus (EBV) infection is characterized by extensive expansion of antigen-specific CD8+ T cells. One potential consequence of this considerable proliferative activity is telomere shortening, which predisposes the EBV-specific cells to replicative senescence. To investigate this, a method was developed that enables the simultaneous identification of EBV specificity of the CD8+ T cells, using major histocompatibility complex (MHC) class I/peptide complexes, together with telomere length, which is determined by fluorescence in situ hybridization. Despite the considerable expansion, CD8+ EBV-specific T cells in patients with AIM maintain their telomere length relative to CD8+ T cells in normal individuals and relative to CD4+ T cells within the patients themselves and this is associated with the induction of the enzyme telomerase. In 4 patients who were studied up to 12 months after resolution of AIM, telomere lengths of EBV-specific CD8+ T cells were unchanged in 3 but shortened in one individual, who was studied only 5 months after initial onset of infection. Substantial telomere shortening in EBV-specific CD8+ T cells was observed in 3 patients who were studied between 15 months and 14 years after recovery from AIM. Thus, although telomerase activation may preserve the replicative potential of EBV-specific cells in AIM and after initial stages of disease resolution, the capacity of these cells to up-regulate this enzyme after restimulation by the persisting virus may dictate the extent of telomere maintenance in the memory CD8+ T-cell pool over time.

Early Impairment of CD8+T Cells Immune Response Against Epstein–Barr Virus (EBV) Antigens Associated with High Level of Circulating Mononuclear EBV DNA Load in HIV Infection

2004 ◽  
Vol 24 (2) ◽  
pp. 125-134 ◽  
Author(s):  
Jérôme Legoff ◽  
Corinne Amiel ◽  
Olivier Calisonni ◽  
Delphine Fromentin ◽  
Bakoliarisoa Rajoely ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Hiv Infection ◽  
Cd8 T Cells ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Ebv Dna ◽  
Epstein Barr ◽  
High Level

Lack of Epstein-Barr virus- and HIV-specific CD27− CD8+ T cells is associated with progression to viral disease in HIV-infection

AIDS ◽  
2002 ◽  
Vol 16 (15) ◽  
pp. 2001-2011 ◽  
Author(s):  
Debbie van Baarle ◽  
Stefan Kostense ◽  
Egbert Hovenkamp ◽  
Graham Ogg ◽  
Nening Nanlohy ◽  
...  
Keyword(s):  
T Cells ◽  
Hiv Infection ◽  
Cd8 T Cells ◽  
Epstein Barr Virus ◽  
Viral Disease ◽  
Barr Virus ◽  
Epstein Barr
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