scholarly journals Comparison of Polymerase Chain Reaction of Polymorphonuclear Leukocytes and Plasma Identifies Patients Who Control Cytomegalovirus Infection after Hematopoietic Cell Transplantation

2008 ◽  
Vol 47 (4) ◽  
pp. 535-539
Author(s):  
Marcy L. Vana ◽  
Danuse Formankova ◽  
Steven Cha ◽  
Anjali Sharma ◽  
Luciano Potena ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cell Transplantation ◽  
Cytomegalovirus Infection ◽  
Polymorphonuclear Leukocytes ◽  
Hematopoietic Cell Transplantation ◽  
Hematopoietic Cell ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals 984. Diagnostic Utility of Bronchoalveolar Lavage Pneumocystis jirovecii DNA Polymerase Chain Reaction Assay in Patients with Hematological Malignancies and Hematopoietic Cell Transplantation

2021 ◽  
Vol 8 (Supplement_1) ◽  
pp. S583-S583
Author(s):  
Deepa Nanayakkara ◽  
Bernard Tegtmeier ◽  
Justine Abella Ross ◽  
Jana Dickter ◽  
Alfredo Puing ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bronchoalveolar Lavage ◽  
Cell Transplantation ◽  
Hematological Malignancies ◽  
Hematopoietic Cell Transplantation ◽  
Hematopoietic Cell ◽  
Pneumocystis Jirovecii ◽  
Chain Reaction ◽  
Fungal Burden ◽  
Polymerase Chain

Abstract Background Pneumocystis jirovecii, an ubiquitous fungus, can lead to opportunistic pneumonia (PJP) in patients with hematological malignancies (HM) and hematopoietic cell transplantation (HCT) with mild to severe presentation. Unlike patients with HIV, diagnosis of PJP pneumonia is often challenging in patients with HM/HCT possibly related to lower fungal burden versus atypical presentation. The gold standard for diagnosis of PJP from bronchoalveolar lavage fluid (BALF) is cytology, followed by direct fluorescent antigen (DFA), however, in the context of lower fungal burden, quantitative polymerase chain reaction (PCR) is increasingly used. PCR DNA load cut-off for diagnosis of PJP is not established. The objective of this study is to assess the correlation between three tests (cytology, DFA and PCR) and diagnosis of PJP (colonization, possible, probable or proven infection). Methods In this retrospective study at City of Hope, HM/HCT patients with BALF performed to investigate pneumonia who tested positive for any of the 3 tests were included. The study period is from July 2014 to July 2020. All patients had a clinical and radiographic diagnosis of pneumonia. Results Eighty-five patients were identified to have at least one positive diagnostic test for PJP. Twenty (23.5%) patients had a PCR with less than 84 copies/mL, and colonization was suspected in these patients. Of the remaining 65 patients, 46 had all 3 tests done. Twenty seven (58.7%) patients only had positive PCR ranging from 106 to 588,000 copies/mL with negative DFA and cytology. Twelve (26.1%) patients had either DFA or cytology positive with a positive PCR, and in 6 patients (13%) all 3 tests were positive. All of these 18 patients had clinical presentation and radiographic findings consistent with PJP. Quantitative or qualitative serum beta-D-glucan (BDG) level was available in 28 patients and 17 had a positive test with a level >80 pg/ml. Conclusion PJP PCR is a very sensitive test that can lead to early detection of PJP pneumonia in HM/HCT with lower sensitivity of DFA/cytology unless the fungal burden is high. However, the optimal cut off PCR value associated with disease needs to be clinically validated in our patient population and a concurrent serum BDG level can increase diagnostic yield. Disclosures All Authors: No reported disclosures

Evaluation of cytomegalovirus infection in low-birth weight children by breast milk using a real-time polymerase chain reaction assay

2015 ◽  
Vol 87 (5) ◽  
pp. 845-850 ◽  
Author(s):  
Romero-Gómez Maria Pilar ◽  
Cabrera Marta ◽  
Montes-Bueno María Teresa ◽  
Cendejas-Bueno Emilio ◽  
Segovia Cristina ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Birth Weight ◽  
Breast Milk ◽  
Low Birth Weight ◽  
Real Time ◽  
Polymerase Chain Reaction Assay ◽  
Cytomegalovirus Infection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Low Birth Weight Children

scholarly journals Peripheral Blood Leukocytes and Serum Nested Polymerase Chain Reaction Are Complementary Methods for Monitoring Active Cytomegalovirus Infection in Transplant Patients

2013 ◽  
Vol 24 (3) ◽  
pp. e69-e74 ◽  
Author(s):  
PD Andrade ◽  
MT Fioravanti ◽  
EBV Anjos ◽  
C De Oliveira ◽  
DM Albuquerque ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Positive Predictive Value ◽  
Predictive Value ◽  
Cytomegalovirus Infection ◽  
Clinical Symptoms ◽  
Peripheral Blood Leukocytes ◽  
Active Infection ◽  
Chain Reaction ◽  
Blood Leukocytes ◽  
Polymerase Chain

BACKGROUND: Human cytomegalovirus is an important cause of morbidity and mortality in immunocompromised patients. Qualitative polymerase chain reaction (PCR) has proven to be a sensitive and effective technique in defining active cytomegalovirus infection, in addition to having low cost and being a useful test for situations in which there is no need for quantification. Real-time PCR has the advantage of quantification; however, the high cost of this methodology makes it impractical for routine use.OBJECTIVE: To apply a nested PCR assay to serum (sPCR) and to evaluate its efficiency to diagnose active cytomegalovirus infection compared with PCR of peripheral blood leukocytes (L-PCR).METHODS: Samples of 37 patients were prospectively evaluated. An internal control was created and applied to sPCR to exclude false-negative results.RESULTS: In total, 21 patients (57%) developed active cytomegalovirus infection. After analyzing the two methods for the diagnosis of active infection, higher sensitivity and negative predictive value of the L-PCR versus sPCR (100% versus 62%), and higher specificity and positive predictive value of sPCR versus L-PCR (81% versus 50% and 72%, respectively) were observed. Discordant results were observed in 11 patients who were L-PCR-positive but sPCR-negative for active cytomegalovirus infection, five of whom developed clinical symptoms of cytomegalovirus. Clinical symptoms were observed in 14 patients, 12 of whom were diagnosed with active infection by nested L-PCR (P=0.007) and seven by nested sPCR (P=0.02). Higher specificity and a positive predictive value for sPCR were observed.CONCLUSION: Nested L-PCR and sPCR were considered to be complementary methods for the diagnosis and management of symptomatic cytomegalovirus infection.

scholarly journals PTU-083 Use Of Polymerase Chain Reaction To Detect Mucosal Cytomegalovirus Infection In Patients With Acute Ulcerative Colitis

Gut ◽  
2014 ◽  
Vol 63 (Suppl 1) ◽  
pp. A75.2-A76 ◽  
Author(s):  
GSZ Tun ◽  
A Wright ◽  
K Robinson ◽  
R Sidhu ◽  
A Hopper ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Polymerase Chain Reaction ◽  
Cytomegalovirus Infection ◽  
Chain Reaction ◽  
Polymerase Chain
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