scholarly journals Whole-Blood Interferon-Gamma Release Assay for Baseline Tuberculosis Screening of Healthcare Workers at a Swiss University Hospital

2008 ◽  
Vol 29 (7) ◽  
pp. 681-683 ◽  
Author(s):  
Ariane Stebler ◽  
Patricia Iseli ◽  
Kathrin Mühlemann ◽  
Thomas Bodmer
Keyword(s):  
Interferon Gamma ◽  
Whole Blood ◽  
Healthcare Workers ◽  
University Hospital ◽  
Interferon Gamma Release Assay ◽  
Tuberculosis Screening ◽  
Release Assay

scholarly journals Diagnosis and Risk Factors of Latent Tuberculosis Infection among Healthcare Workers Using Whole Blood Human Interferon-gamma Release Assay and Tuberculin Skin Testing

2020 ◽  
pp. 15-21
Author(s):  
A. N. Umo ◽  
O. J. Akinjogunla ◽  
N. O. Umoh ◽  
G. E. Uzono
Keyword(s):  
Risk Factors ◽  
Interferon Gamma ◽  
Whole Blood ◽  
Healthcare Workers ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Interferon Gamma Release Assay ◽  
Tuberculin Skin Testing ◽  
Release Assay

This study established the diagnosis and risk factors of latent tuberculosis infection (LTBI) among health-care workers in an endemic population using Tuberculin skin test (TST) and Quantiferon TB-gold. A total of 609 Healthcare workers from tuberculosis treatment facilities in Akwa Ibom State, Nigeria were studied. The Interferon-gamma release assay was performed using 3ml of whole blood by ELISA according to the manufacturer’s instruction (Cellestis Ltd., Carnegie, Australia) after which 0.1 ml of 5 tuberculin units of Purified Protein Derivative (PPD) was administered intra-dermally to each subject. TST results were read after 72 hours by measuring the size of indurations in millimetres. Data were analysed using SPSS version 17 (SPSS Inc., Chicago, Illinois). At the threshold of 10 mm, the prevalence of LTBI by TST was 45.8% and 24.8% at the IGRA diagnostic value of ≥ 0.351 IU. Laboratory staff and ward orderlies as well as being in service for >10 years, were more significantly associated with LTBI. A moderate agreement of 76.7%, k = 0.51 was obtained between TST at 10 mm, and QFT. Neither previous exposure to TST nor BCG vaccination affected the prevalence of LTBI in the study population. The difference of 54% prevalence of LTBI between TST and QFT may be due to non-tuberculous mycobacterium (NTM) since TST is non-specific. This may have grave implications of drug toxicity and development of resistance to anti-TB drug among individuals harbouring NTM, but receiving anti-TB medication. The 76.7% agreement between the two tests is an indication that the 10 mm cut-off induration for TST is still relevant in the diagnosis of LTBI.

scholarly journals Whole-Blood Interferon-Gamma Release Assay for Diagnosis of Tuberculous Lymphadenitis

2011 ◽  
Vol 224 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Young Keun Kim ◽  
Young Uh ◽  
Nam Seok Lee ◽  
Mee Yon Cho ◽  
Minseob Eom ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Whole Blood ◽  
Interferon Gamma Release Assay ◽  
Tuberculous Lymphadenitis ◽  
Release Assay

Negative Tuberculin Skin Test and Prediction of Reversion of QuantiFERON Interferon Gamma Release Assay in US Healthcare Workers

2016 ◽  
Vol 37 (4) ◽  
pp. 478-482 ◽  
Author(s):  
Wendy Thanassi ◽  
Art Noda ◽  
Beatriz Hernandez ◽  
Leah Friedman ◽  
Susan Dorman ◽  
...  
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Interferon Gamma ◽  
Healthcare Workers ◽  
Low Risk ◽  
Infect Control Hosp ◽  
Skin Tests ◽  
Interferon Gamma Release Assay ◽  
Negative Tuberculin Skin Test ◽  
Release Assay

QuantiFERON tuberculosis tests (QFT) reverted in (612) 77% of 1,094 low-risk healthcare workers (HCW) testing less than 1.16 IU/mL. Of HCW testing greater than 1.1 IU/mL, 33 (59%) of 56 with negative tuberculin skin tests (TST) reverted vs 8 (6%) of 125 with positive TSTs. Retesting low-risk QFT-positive and TST-negative HCW is prudent.Infect. Control Hosp. Epidemiol. 2016;37(4):478–482

Usefulness of the whole-blood interferon-gamma release assay for diagnosis of extrapulmonary tuberculosis

2009 ◽  
Vol 63 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Kyoung-Ho Song ◽  
Jae Hyun Jeon ◽  
Wan Beom Park ◽  
Sung-Han Kim ◽  
Kyoung Un Park ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Whole Blood ◽  
Extrapulmonary Tuberculosis ◽  
Interferon Gamma Release Assay ◽  
Release Assay
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