scholarly journals Plasma Levels of Monocyte Chemoattractant Protein‐1 but Not Those of Macrophage Inhibitory Protein‐1a and RANTES Correlate with Virus Load in Human Immunodeficiency Virus Infection

1997 ◽  
Vol 176 (6) ◽  
pp. 1621-1624 ◽  
Author(s):  
Laurence Weiss ◽  
Ali Si‐Mohamed ◽  
Philippe Giral ◽  
Philippe Castiel ◽  
Annick Ledur ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Virus Infection ◽  
Human Immunodeficiency Virus Infection ◽  
Plasma Levels ◽  
Inhibitory Protein ◽  
Monocyte Chemoattractant Protein 1 ◽  
Virus Load ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Immunodeficiency Virus

scholarly journals The Human Immunodeficiency Virus Type 1 Tat Protein Up-Regulates the Promoter Activity of the Beta-Chemokine Monocyte Chemoattractant Protein 1 in the Human Astrocytoma Cell Line U-87 MG: Role of SP-1, AP-1, and NF-κB Consensus Sites

2000 ◽  
Vol 74 (4) ◽  
pp. 1632-1640 ◽  
Author(s):  
Siew Pheng Lim ◽  
Alfredo Garzino-Demo
Keyword(s):  
Human Immunodeficiency Virus ◽  
Promoter Activity ◽  
Tat Protein ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Astrocytoma Cell ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT It has been shown that the human immunodeficiency virus type 1 (HIV-1) Tat protein can specifically enhance expression and release of monocyte chemoattractant protein 1 (MCP-1) from human astrocytes. In this study, we show evidence that Tat-induced MCP-1 expression is mediated at the transcriptional level. Transient transfection of an expression construct encoding the full-length Tat into the human glioblastoma-astrocytoma cell line U-87 MG enhances reporter gene activity from cotransfected deletion constructs of the MCP-1 promoter. HIV-1 Tat exerts its effect through a minimal construct containing 213 nucleotides upstream of the translational start site. Site-directed mutagenesis studies indicate that an SP1 site (located between nucleotides −123 and −115) is critical for both constitutive and Tat-enhanced expression of the human MCP-1 promoter, as mutation of this SP1 site significantly diminished reporter gene expression in both instances. Gel retardation experiments further demonstrate that Tat strongly enhances the binding of SP1 protein to its DNA element on the MCP-1 promoter. Moreover, we also observe an increase in the binding activities of transcriptional factors AP1 and NF-κB to the MCP-1 promoter following Tat treatment. Mutagenesis studies show that an upstream AP1 site and an adjacent NF-κB site (located at −128 to −122 and −150 to −137, respectively) play a role in Tat-mediated transactivation. In contrast, a further upstream AP1 site (−156 to −150) does not appear to be crucial for promoter activity. We postulate that a Tat-mediated increase in SP1 binding activities augments the binding of AP1 and NF-κB, leading to synergistic activation of the MCP-1 promoter.

scholarly journals Correlation of Virus Load in Plasma and Lymph Node Tissue in Human Immunodeficiency Virus Infection

1997 ◽  
Vol 176 (5) ◽  
pp. 1388-1392 ◽  
Author(s):  
Marianne Harris ◽  
Pierre Patenaude ◽  
Peter Cooperberg ◽  
Douglas Filipenko ◽  
Anona Thorne ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Lymph Node ◽  
Virus Infection ◽  
Human Immunodeficiency Virus Infection ◽  
Virus Load ◽  
Immunodeficiency Virus

scholarly journals Stability of Plasma Levels of Cytokines and Soluble Activation Markers in Patients with Human Immunodeficiency Virus Infection

1999 ◽  
Vol 179 (4) ◽  
pp. 843-848 ◽  
Author(s):  
Najib Aziz ◽  
Parunag Nishanian ◽  
Jeremy M. G. Taylor ◽  
Ronald T. Mitsuyasu ◽  
Jeffrey M. Jacobson ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Virus Infection ◽  
Human Immunodeficiency Virus Infection ◽  
Plasma Levels ◽  
Activation Markers ◽  
Immunodeficiency Virus
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