scholarly journals Isolation ofLegionella pneumophilaSerogroup 1 from Human Feces with Use of Amebic Cocultures

1998 ◽  
Vol 26 (2) ◽  
pp. 502-503 ◽  
Author(s):  
Timothy J. Rowbotham
Keyword(s):  
Human Feces

scholarly journals Recovery of SARS-CoV-2 from Wastewater Using Centrifugal Ultrafiltration

2021 ◽  
Vol 4 (2) ◽  
pp. 32
Author(s):  
Brienna L. Anderson-Coughlin ◽  
Adrienne E. H. Shearer ◽  
Alexis N. Omar ◽  
K. Eric Wommack ◽  
Kalmia E. Kniel
Keyword(s):  
Enteric Virus ◽  
Clinical Testing ◽  
Human Feces ◽  
Angiotensin Converting Enzyme 2 ◽  
Human Waste ◽  
Safety Research ◽  
New Castle County ◽  
Wastewater Systems ◽  
High Concentrations ◽  
Centrifugal Ultrafiltration

The COVID-19 pandemic is a global crisis and continues to impact communities as the disease spreads. Clinical testing alone provides a snapshot of infected individuals but is costly and difficult to perform logistically across whole populations. The virus which causes COVID-19, SARS-CoV-2, is shed in human feces and urine and can be detected in human waste. SARS-CoV-2 can be shed in high concentrations (>107 genomic copies/mL) due to its ability to replicate in the gastrointestinal tract of humans through attachment to the angiotensin-converting enzyme 2 (ACE-2) receptors there. Monitoring wastewater for SARS-CoV-2, alongside clinical testing, can more accurately represent the spread of disease within a community. This protocol describes a reliable and efficacious method to recover SARS-CoV-2 in wastewater, quantify genomic RNA levels, and evaluate concentration fluctuations over time. Using this protocol, viral levels as low as 10 genomic copies/mL were successfully detected from 30 mL of wastewater in more than seven-hundred samples collected between August 2020 and March 2021. Through the adaptation of traditional enteric virus methods used in food safety research, targets have been reliably detected with no inhibition of detection (RT-qPCR) observed in any sample processed. This protocol is currently used for surveillance of wastewater systems across New Castle County, Delaware.

Coprobacter secundus subsp. similis subsp. nov. and Solibaculum mannosilyticum gen. nov., sp. nov., isolated from human feces

2021 ◽  
Author(s):  
Mitsuo Sakamoto ◽  
Nao Ikeyama ◽  
Atsushi Toyoda ◽  
Takumi Murakami ◽  
Hiroshi Mori ◽  
...  
Keyword(s):  
Human Feces

scholarly journals Discrimination of Viable and Dead Fecal Bacteroidales Bacteria by Quantitative PCR with Propidium Monoazide

2009 ◽  
Vol 75 (9) ◽  
pp. 2940-2944 ◽  
Author(s):  
Sungwoo Bae ◽  
Stefan Wuertz
Keyword(s):  
Quantitative Pcr ◽  
Light Exposure ◽  
Bacteroides Fragilis ◽  
Extracellular Dna ◽  
Propidium Monoazide ◽  
Human Feces ◽  
Heat Treated ◽  
Pcr Conditions

ABSTRACT Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 μM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.

Purification of Hepatitis A Virus from Human Feces

Intervirology ◽  
1978 ◽  
Vol 10 (5) ◽  
pp. 300-308 ◽  
Author(s):  
Stephen A. Locarnini ◽  
Anthony G. Coulepis ◽  
Allan A. Ferris ◽  
Noreen I. Lehmann ◽  
Ian D. Gust
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Human Feces

Increased Butyrate Production During Long-Term Fermentation ofIn Vitro-Digested High Amylose Cornstarch Residues with Human Feces

2015 ◽  
Vol 80 (9) ◽  
pp. M1997-M2004 ◽  
Author(s):  
Li Li ◽  
Hongxin Jiang ◽  
Hyun-Jung Kim ◽  
Man-Yu Yum ◽  
Mark R. Campbell ◽  
...  
Keyword(s):  
Human Feces ◽  
High Amylose ◽  
Butyrate Production
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