scholarly journals Limulus Amebocyte Lysate Assay for Detection of Endotoxin in Patients with Sepsis Syndrome

1998 ◽  
Vol 27 (3) ◽  
pp. 582-591 ◽  
Author(s):  
D. W. Bates ◽  
J. Parsonnet ◽  
P. A. Ketchum ◽  
E. B. Miller ◽  
T. J. Novitsky ◽  
...  
Keyword(s):  
Sepsis Syndrome ◽  
Limulus Amebocyte Lysate ◽  
Limulus Amebocyte Lysate Assay

Pyrogenic Reactions Associated With Single Daily Dosing of Intravenous Gentamicin

2000 ◽  
Vol 21 (12) ◽  
pp. 771-774 ◽  
Author(s):  
Udo Buchholz ◽  
Chesley Richards ◽  
Rekha Murthy ◽  
Matthew Arduino ◽  
Doreen Pon ◽  
...  
Keyword(s):  
Los Angeles ◽  
Community Hospital ◽  
Practice Change ◽  
Limulus Amebocyte Lysate ◽  
Factors Associated ◽  
Epidemic Period ◽  
Dosing Intervals ◽  
Parenteral Products ◽  
Clinical Practice Change ◽  
Limulus Amebocyte Lysate Assay

Objective:To identify risk factors associated with an unexpected outbreak of pyrogenic reactions (PR) following intravenous gentamicin.Design:We conducted two cohort studies. PRs were defined as chills, rigors, or shaking within 3 hours after initiating the gentamicin infusion during the preepidemic (December 1, 1997-January 15,1998) or epidemic (May 1-June 15,1998) periods. We tested gentamicin vials for endotoxin using the limulus amebocyte lysate assay.Setting:Inpatient services of a large community hospital in Los Angeles, California.Results:During the epidemic period, 22 (15%) of 152 patients developed documented PRs following intravenous gentamicin. PRs were more likely among patients receiving single daily dosing (SDD) than multiple daily dosing gentamicin (20/73 [27%] vs 2/79 [3%]; relative risk, 10.8; 95% confidence interval, 2.644.7). Laboratory analysis of gentamicin vials found endotoxin levels that were higher among Fujisawa-brand gentamicin (implicated brand) than gentamicin used after the outbreak terminated (non-implicated brand). Although endotoxin levels in the vials did not exceed US Pharmacopeia limits (1.7 endotoxin units/mg gentamicin), the use of SDD gentamicin may place patients at greater risk of receiving doses of endotoxin above the threshold for PRs in humans.Conclusions:Reassessment of the acceptable amounts of endotoxin in gentamicin and other parenteral products should be considered when dosing intervals used in clinical practice change.

scholarly journals Quantitative lipopolysaccharide analysis using HPLC/MS/MS and its combination with the limulus amebocyte lysate assay

2015 ◽  
Vol 56 (7) ◽  
pp. 1363-1369 ◽  
Author(s):  
Jean-Paul Pais de Barros ◽  
Thomas Gautier ◽  
Wahib Sali ◽  
Christophe Adrie ◽  
Hélène Choubley ◽  
...  
Keyword(s):  
Limulus Amebocyte Lysate ◽  
Limulus Amebocyte Lysate Assay

scholarly journals Detection of Microbial Translocation in HIV and SIV Infection Using the Limulus Amebocyte Lysate Assay is Masked by Serum and Plasma

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e41258 ◽  
Author(s):  
Ashwin Balagopal ◽  
Lucio Gama ◽  
Veronica Franco ◽  
Julia N. Russell ◽  
Jeffrey Quinn ◽  
...  
Keyword(s):  
Microbial Translocation ◽  
Limulus Amebocyte Lysate ◽  
Siv Infection ◽  
Limulus Amebocyte Lysate Assay
Sign in / Sign up

Export Citation Format

Share Document