scholarly journals Detection of Enterocytozoon bieneusi in Fecal Specimens by Polymerase Chain Reaction Analysis with Primers to the Small‐Subunit rRNA

1998 ◽  
Vol 26 (3) ◽  
pp. 673-675 ◽  
Author(s):  
Andrew H. Talal ◽  
Donald P. Kotler ◽  
Jan M. Orenstein ◽  
Louis M. Weiss
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Analysis ◽  
Small Subunit ◽  
Enterocytozoon Bieneusi ◽  
Small Subunit Rrna ◽  
Chain Reaction ◽  
Polymerase Chain

Detection and identification ofEntamoeba gingivalisby specific amplification of rRNA gene

1996 ◽  
Vol 42 (12) ◽  
pp. 1248-1251 ◽  
Author(s):  
Noriko Kikuta ◽  
Ayako Yamamoto ◽  
Nobuichi Goto
Keyword(s):  
Polymerase Chain Reaction ◽  
Protozoan Parasite ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Chain Reaction ◽  
Gene Encoding ◽  
Detection And Identification ◽  
Specific Amplification ◽  
Polymerase Chain

A pair of oligonucleotide primers were designed from the nucleotide sequence of the gene encoding the small subunit ribosomal RNA (SrRNA) of the oral protozoan parasite Entamoeba gingivalis. The primers amplified a 1.4-kb DNA fragment by polymerase chain reaction and were specific for Entamoeba gingivalis but not for other protozoa, oral protists and bacteria, or human leukocytes. With this method, the DNA from as few as 30 cells of Entamoeba gingivalis could be detected. These results suggest that this approach is applicable to the detection and identification of Entamoeba gingivalis in the human oral cavity.Key words: Entamoeba gingivalis, small subunit rRNA, polymerase chain reaction, diagnostics.

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