scholarly journals Vδ2+γδ T Cell Function inMycobacterium tuberculosis–and HIV‐1–Positive Patients in the United States and Uganda: Application of a Whole‐Blood Assay

2005 ◽  
Vol 192 (10) ◽  
pp. 1806-1814 ◽  
Author(s):  
Roxana E. Rojas ◽  
Keith A. Chervenak ◽  
Jeremy Thomas ◽  
Jamila Morrow ◽  
Lorna Nshuti ◽  
...  
Keyword(s):  
United States ◽  
T Cell ◽  
Whole Blood ◽  
Cell Function ◽  
The United States ◽  
Γδ T Cell ◽  
Whole Blood Assay ◽  
Blood Assay ◽  
T Cell Function ◽  
Hiv 1

scholarly journals Expansion of Monocytic Myeloid-Derived Suppressor Cells Dampens T Cell Function in HIV-1-Seropositive Individuals

2012 ◽  
Vol 87 (3) ◽  
pp. 1477-1490 ◽  
Author(s):  
Aiping Qin ◽  
Weiping Cai ◽  
Ting Pan ◽  
Kang Wu ◽  
Qiong Yang ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Function ◽  
Suppressor Cells ◽  
Cell Contact ◽  
Myeloid Derived Suppressor Cells ◽  
Cell Dysfunction ◽  
Arginase 1 ◽  
T Cell Function ◽  
T Cell Dysfunction ◽  
Hiv 1

ABSTRACTT lymphocyte dysfunction contributes to human immunodeficiency virus type 1 (HIV-1) disease progression by impairing antivirus cellular immunity. However, the mechanisms of HIV-1 infection-mediated T cell dysfunction are not completely understood. Here, we provide evidence that expansion of monocytic myeloid-derived suppressor cells (M-MDSCs) suppressed T cell function in HIV-1-infected individuals. We observed a dramatic elevation of M-MDSCs (HLA-DR−/lowCD11b+CD33+/highCD14+CD15−cells) in the peripheral blood of HIV-1-seropositive subjects (n= 61) compared with healthy controls (n= 51), despite efficacious antiretroviral therapy for nearly 2 years. The elevated M-MDSC frequency in HIV-1+subjects correlated with prognostic HIV-1 disease markers, including the HIV-1 load (r= 0.5957;P< 0.0001), CD4+T cell loss (r= −0.5312;P< 0.0001), and activated T cells (r= 0.4421;P= 0.0004). Functional studies showed that M-MDSCs from HIV-1+subjects suppressed T cell responses in both HIV-1-specific and antigen-nonspecific manners; this effect was dependent on the induction of arginase 1 and required direct cell-cell contact. Further investigations revealed that direct HIV-1 infection or culture with HIV-1-derived Tat protein significantly enhanced human MDSC generationin vitro, and MDSCs from healthy donors could be directly infected by HIV-1 to facilitate HIV-1 replication and transmission, indicating that a positive-feedback loop between HIV-1 infection and MDSC expansion existed. In summary, our studies revealed a novel mechanism of T cell dysfunction in HIV-1-infected individuals and suggested that targeting MDSCs may be a promising strategy for HIV-1 immunotherapy.

Covalent Inhibitors of Interleukin-2 Inducible T Cell Kinase (Itk) with Nanomolar Potency in a Whole-Blood Assay

2012 ◽  
Vol 55 (22) ◽  
pp. 10047-10063 ◽  
Author(s):  
Christoph W. Zapf ◽  
Brian S. Gerstenberger ◽  
Li Xing ◽  
David C. Limburg ◽  
David R. Anderson ◽  
...  
Keyword(s):  
T Cell ◽  
Whole Blood ◽  
Interleukin 2 ◽  
Whole Blood Assay ◽  
Blood Assay ◽  
Covalent Inhibitors ◽  
Inducible T Cell Kinase

scholarly journals Neutrophils suppress γδ T-cell function

2013 ◽  
Vol 44 (3) ◽  
pp. 819-830 ◽  
Author(s):  
Florencia Sabbione ◽  
María L. Gabelloni ◽  
Glenda Ernst ◽  
María S. Gori ◽  
Gabriela Salamone ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Function ◽  
Γδ T Cell ◽  
T Cell Function

scholarly journals Stable expression of transdominant Rev protein in human T cells inhibits human immunodeficiency virus replication.

1992 ◽  
Vol 176 (4) ◽  
pp. 1197-1201 ◽  
Author(s):  
M H Malim ◽  
W W Freimuth ◽  
J Liu ◽  
T J Boyle ◽  
H K Lyerly ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
T Cell ◽  
Hiv Infection ◽  
Viral Replication ◽  
Cell Lines ◽  
Cell Function ◽  
T Cell Function ◽  
Immunodeficiency Virus ◽  
Hiv 1 ◽  
Rev Protein

The human immunodeficiency virus (HIV) Rev protein is essential for viral structural protein expression (Gag, Pol, and Env) and, hence, for viral replication. In transient transfection assays, mutant forms of Rev have been identified that inhibit wild-type Rev activity and therefore suppress viral replication. To determine whether such transdominant Rev proteins could provide long-term protection against HIV infection without affecting T cell function, T leukemia cell lines were stably transduced with a retroviral vector encoding a transdominant mutant of the Rev protein, M10. While all the M10-expressing cell lines remained infectable by HIV-1, these same cells failed to support a productive replication cycle when infected with a cloned isolate of HIV-1. In addition, two out of three M10-expressing CEM clones were also resistant to highly productive infection by a heterogeneous HIV-1 pool. Expression of M10 did not affect induction of HIV transcription mediated by the kappa B regulatory element or Tat. Importantly, constitutive expression of Rev M10 did not alter the secretion of interleukin 2 in response to mitogen stimulation of EL-4 and Jurkat cells. The inhibition of HIV infection in cells stably expressing a transdominant Rev protein, in the absence of any deleterious effect on T cell function, suggests that such a strategy could provide a therapeutic effect in the T lymphocytes of acquired immunodeficiency syndrome patients.

scholarly journals Ligand recognition during thymic development and γδ T cell function specification

2010 ◽  
Vol 22 (4) ◽  
pp. 207-213 ◽  
Author(s):  
Christina Meyer ◽  
Xun Zeng ◽  
Yueh-hsiu Chien
Keyword(s):  
T Cell ◽  
Cell Function ◽  
Ligand Recognition ◽  
Γδ T Cell ◽  
Thymic Development ◽  
T Cell Function

scholarly journals Erratum: Corrigendum: The differential short- and long-term effects of HIV-1 latency-reversing agents on T cell function

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
G. Clutton ◽  
Y. Xu ◽  
P. L. Baldoni ◽  
K. R. Mollan ◽  
J. Kirchherr ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Function ◽  
Long Term Effects ◽  
T Cell Function ◽  
Short And Long Term ◽  
Hiv 1 ◽  
Reversing Agents
Sign in / Sign up

Export Citation Format

Share Document